Development of a Single Indicator of Facial Skin Aging and Youthful Plump Appearance; Ageless-U and Its Use for Evaluating the Efficacy of Treatment with Galactomyces Ferment Filtrate-Containing Anti-Aging Skin Care Formula
Background/Objective: Wrinkles, nasolabial folds, pigmented spots, and roughness are representative parameters reflecting facial skin aging. Among them, nasolabial folds are a particular concern associated with aging. However, changes in the skin surface topography on the cheek above these folds remain unclear. This research aimed to develop a dynamic multi-angle imaging method and a single indicator of skin aging to quantify both the aged and youthful plump appearance of the skin. Methods: A facial imaging system was used in two separate studies to capture images from multiple angles. An image analysis algorithm quantified facial lines and two diagonal facial lines from the outer and inner cheek regions, forming a U-shaped line. Block analysis computed the average slope and intercept of line segments, and an excircle with its center coordinate was calculated from the U-shaped line. The elevation of this coordinate on the cheek, termed “Ageless-U,” served as a single indicator representing the severity of sagging of aging-related facial lines and the youthful appearance in a wide angle of view of the facial skin. Eighty-five East Asian women aged 22 - 57 participated in Study 1 to examine relationships between Ageless-U, age, skin elasticity, and visual signs of aging. In Study 2, 35 East Asian women aged 32 - 54 applied a Galactomyces ferment filtrate-containing anti-aging skincare product twice daily for 4 weeks, with measurements at baseline, week 2, and week 4 to determine this product’s efficacy against facial aging. Results: In Study 1, Ageless-U significantly descended with increasing age and was associated with reduced skin elasticity and hydration, with notable declines in the 20s and 40s and its position was significantly lowered from the upper cheek to lower cheek. In Study 2, daily treatment elevated Ageless-U at both 2 and 4 weeks. Conclusion: Ageless-U is an early indicator representing topographic features of the skin aging on the face, and the youthful plump skin appearance at the same time. Ageless-U descent can be prevented and reversed by the appropriate treatment of anti-aging skincare products.
References
[1]
Akazaki, S., Nakagawa, H., Kazama, H., Osanai, O., Kawai, M., Takema, Y., et al. (2002) Age-Related Changes in Skin Wrinkles Assessed by a Novel Three-Dimensional Morphometric Analysis. British Journal of Dermatology, 147, 689-695. https://doi.org/10.1046/j.1365-2133.2002.04874.x
[2]
Kakudo, N., Kushida, S., Tanaka, N., Minakata, T., Suzuki, K. and Kusumoto, K. (2011) A Novel Method to Measure Conspicuous Facial Pores Using Computer Analysis of Digital‐Camera‐Captured Images: The Effect of Glycolic Acid Chemical Peeling. Skin Research and Technology, 17, 427-433. https://doi.org/10.1111/j.1600-0846.2011.00514.x
[3]
Miyamoto, K., Takiwaki, H., Hillebrand, G.G. and Arase, S. (2002) Development of a Digital Imaging System for Objective Measurement of Hyperpigmented Spots on the Face. Skin Research and Technology, 8, 227-235. https://doi.org/10.1034/j.1600-0846.2002.00325.x
[4]
Takiwaki, H. (2002) Instrumental Quantification and Its Clinical Application in the Future. Skin Research and Technology, 8, 71-72. https://doi.org/10.1034/j.1600-0846.2002.00344.x
[5]
Miyamoto, K., Inoue, Y., Hsueh, K., Liang, Z., Yan, X., Yoshii, T., et al. (2011) Characterization of Comprehensive Appearances of Skin Ageing: An 11-Year Longitudinal Study on Facial Skin Ageing in Japanese Females at Akita. Journal of Dermatological Science, 64, 229-236. https://doi.org/10.1016/j.jdermsci.2011.09.009
[6]
Flament, F., Jacquet, L., Ye, C., Amar, D., Kerob, D., Jiang, R., et al. (2022) Artificial Intelligence Analysis of over Half a Million European and Chinese Women Reveals Striking Differences in the Facial Skin Ageing Process. Journal of the European Academy of Dermatology and Venereology, 36, 1136-1142. https://doi.org/10.1111/jdv.18073
[7]
Miyamoto, K., Inoue, Y., Yan, X., Yagi, S., Suda, S. and Furue, M. (2023) Significant Reversal of Facial Wrinkle, Pigmented Spot and Roughness by Daily Application of Galactomyces Ferment Filtrate-Containing Skin Products for 12 Months—An 11-Year Longitudinal Skin Aging Rejuvenation Study. Journal of Clinical Medicine, 12, Article 1168. https://doi.org/10.3390/jcm12031168
[8]
Swift, A., Liew, S., Weinkle, S., Garcia, J.K. and Silberberg, M.B. (2020) The Facial Aging Process from the “Inside Out”. Aesthetic Surgery Journal, 41, 1107-1119. https://doi.org/10.1093/asj/sjaa339
[9]
Tanaka, O.M., Cavassin, L.D., Gasparello, G.G., Meira, T.M., Miyoshi, C.S. and Hartmann, G.C. (2022) The Esthetics of the Nasolabial Fold and Age in the Elderly via Eye-Tracking. Contemporary Clinical Dentistry, 14, 18-24. https://doi.org/10.4103/ccd.ccd_539_21
[10]
Takei, K., Mitoma, C., Hashimoto‐Hachiya, A., Takahara, M., Tsuji, G., Nakahara, T., et al. (2015) Galactomyces Fermentation Filtrate Prevents T Helper 2‐Mediated Reduction of Filaggrin in an Aryl Hydrocarbon Receptor‐Dependent Manner. Clinical and Experimental Dermatology, 40, 786-793. https://doi.org/10.1111/ced.12635
[11]
Hashimoto-Hachiya, A., Tsuji, G. and Furue, M. (2019) Antioxidants Cinnamaldehyde and Galactomyces Fermentation Filtrate Downregulate Senescence Marker CDKN2A/p16INK4A via NRF2 Activation in Keratinocytes. Journal of Dermatological Science, 96, 53-56. https://doi.org/10.1016/j.jdermsci.2019.09.002
[12]
Pang, J., Wong, W., Hakozaki, T., Yoshii, T. and Chen, T. (2011) Up-Regulation of Tight Junction-Related Proteins and Increase of Human Epidermal Keratinocytes Barrier Function by Saccharomycosis Ferment Filtrate. Journal of Cosmetics, Dermatological Sciences and Applications, 1, 15-24. https://doi.org/10.4236/jcdsa.2011.11003
[13]
Kataoka, S., Hattori, K., Date, A. and Tamura, H. (2013) Human Keratinocyte Caspase-14 Expression Is Altered in Human Epidermal 3D Models by Dexamethasone and by Natural Products Used in Cosmetics. Archives of Dermatological Research, 305, 683-689. https://doi.org/10.1007/s00403-013-1359-0
[14]
Furue, M., Uchi, H., Mitoma, C., Hashimoto-Hachiya, A., Chiba, T., Ito, T., et al. (2017) Antioxidants for Healthy Skin: The Emerging Role of Aryl Hydrocarbon Receptors and Nuclear Factor-Erythroid 2-Related Factor-2. Nutrients, 9, Article 223. https://doi.org/10.3390/nu9030223
[15]
Miyamoto, K., Dissanayake, B., Omotezako, T., Takemura, M., Tsuji, G. and Furue, M. (2021) Daily Fluctuation of Facial Pore Area, Roughness and Redness among Young Japanese Women; Beneficial Effects of Galactomyces Ferment Filtrate Containing Antioxidative Skin Care Formula. Journal of Clinical Medicine, 10, Article 2502. https://doi.org/10.3390/jcm10112502
[16]
Miyamoto, K., Munakata, Y., Yan, X., Tsuji, G. and Furue, M. (2022) Enhanced Fluctuations in Facial Pore Size, Redness, and TEWL Caused by Mask Usage Are Normalized by the Application of a Moisturizer. Journal of Clinical Medicine, 11, Article 2121. https://doi.org/10.3390/jcm11082121
[17]
Manosroi, A., Chutoprapat, R., Abe, M., Manosroi, W. and Manosroi, J. (2012) Anti-Aging Efficacy of Topical Formulations Containing Niosomes Entrapped with Rice Bran Bioactive Compounds. Pharmaceutical Biology, 50, 208-224. https://doi.org/10.3109/13880209.2011.596206
[18]
McCall-Perez, F., Stephens, T.J. and Herndon Jr., J.H. (2011) Efficacy and Tolera-Bility of a Facial Serum for Fine Lines, Wrinkles, and Photodamaged Skin. Journal of Clinical and Aesthetic Dermatology, 4, 51-54.
[19]
Gold, M.H., Hu, J.Y., Biron, J.A., Yatskayer, M., Dahl, A. and Oresajo, C. (2011) Tolerability and Efficacy of Retinoic Acid Given After Full-Face Peel Treatment of Photodamaged Skin. Journal of Clinical and Aesthetic Dermatology, 4, 40-48.
[20]
Tsukahara, K., Sugata, K., Osanai, O., Ohuchi, A., Miyauchi, Y., Takizawa, M., et al. (2007) Comparison of Age-Related Changes in Facial Wrinkles and Sagging in the Skin of Japanese, Chinese and Thai Women. Journal of Dermatological Science, 47, 19-28. https://doi.org/10.1016/j.jdermsci.2007.03.007
[21]
Lugaresi, C., Tang, J., Nash, H., McClanahan, C., Uboweja, E., Hays, M., Zhang, F., Chang, C.L., Yong, M.G., Lee, J., Chang, W.T., Hua, W., Georg, M. and Grund-Mann, M. (2019) Media Pipe: A Framework for Building Perception Pipelines. Preprintarxiv:1906.08172.
[22]
Miyamoto, K., Dissanayake, B., Wanigasekara, S., Fujii, K., Yan, X. and Furue, M. (2023) Expanded Follicle-Sulcus-Crack Complex Is an Early Warning Sign of Facial Skin Aging: Improvement by Application of Galactomyces Ferment Filtrate-Containing Skin Product. Journal of Cosmetics, Dermatological Sciences and Applications, 13, 91-106. https://doi.org/10.4236/jcdsa.2023.132010
[23]
Miyamoto, K., Nagasawa, H., Inoue, Y., Nakaoka, K., Hirano, A. and Kawada, A. (2012) Development of New in Vivo Imaging Methodology and System for the Rapid and Quantitative Evaluation of the Visual Appearance of Facial Skin Firmness. Skin Research and Technology, 19, 525-e531. https://doi.org/10.1111/srt.12005
[24]
Krueger, N., Luebberding, S., Oltmer, M., Streker, M. and Kerscher, M. (2011) Age‐Related Changes in Skin Mechanical Properties: A Quantitative Evaluation of 120 Female Subjects. Skin Research and Technology, 17, 141-148. https://doi.org/10.1111/j.1600-0846.2010.00486.x
[25]
Ezure, T., Yagi, E., Kunizawa, N., Hirao, T. and Amano, S. (2011) Comparison of Sagging at the Cheek and Lower Eyelid between Male and Female Faces. Skin Research and Technology, 17, 510-515. https://doi.org/10.1111/j.1600-0846.2011.00526.x
[26]
Virtanen, P., Gommers, R., Oliphant, T.E., Haberland, M., Reddy, T., Cournapeau, D., et al. (2020) Author Correction: SciPy 1.0: Fundamental Algorithms for Scientific Computing in Python. Nature Methods, 17, 352-352. https://doi.org/10.1038/s41592-020-0772-5
[27]
Lee, Y.I., Choi, S., Roh, W.S., Lee, J.H. and Kim, T. (2021) Cellular Senescence and Inflammaging in the Skin Microenvironment. International Journal of Molecular Sciences, 22, Article 3849. https://doi.org/10.3390/ijms22083849
[28]
Pilkington, S.M., Bulfone-Paus, S., Griffiths, C.E.M. and Watson, R.E.B. (2021) Inflammaging and the Skin. Journal of Investigative Dermatology, 141, 1087-1095. https://doi.org/10.1016/j.jid.2020.11.006
[29]
Park, J.W., Lee, M., Kim, J. and Kim, E. (2020) Quantitative Evaluation of Facial Sagging in Different Body Postures Using a Three‐Dimensional Imaging Technique. Journal of Cosmetic Dermatology, 20, 2583-2592. https://doi.org/10.1111/jocd.13880
[30]
Tanaka, Y. (2020) Three-Dimensional Quantification of Skin Surface Displacement Following Skin Rejuvenation Using Solar Protection and Solar Repair. The Journal of Clinical and Aesthetic Dermatology, 13, 47-50.
[31]
Ohshima, H., Kinoshita, S., Oyobikawa, M., Futagawa, M., Takiwaki, H., Ishiko, A., et al. (2012) Use of Cutometer Area Parameters in Evaluating Age‐Related Changes in the Skin Elasticity of the Cheek. Skin Research and Technology, 19, 238-e242. https://doi.org/10.1111/j.1600-0846.2012.00634.x
[32]
Ahn, S., Kim, S., Lee, H., Moon, S. and Chang, I. (2007) Correlation between a Cutometer® and Quantitative Evaluation Using Moire Topography in Age‐Related Skin Elasticity. Skin Research and Technology, 13, 280-284. https://doi.org/10.1111/j.1600-0846.2007.00224.x
[33]
Kuwazuru, O., Miyamoto, K., Yoshikawa, N. and Imayama, S. (2012) Skin Wrinkling Morphology Changes Suddenly in the Early 30s. Skin Research and Technology, 18, 495-503. https://doi.org/10.1111/j.1600-0846.2011.00598.x