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基于荧光硅纳米粒子的pH响应型复合纳米药物载体的制备及其在肿瘤细胞中的应用
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Abstract:
一锅水热法制备的硅纳米粒子(Si NPs)作为荧光载体，介孔二氧化硅纳米粒子(MSNs)为药物载体，制备出了pH响应型复合荧光纳米药物载体(Si NPs-MSNs)，并针对肿瘤细胞进行荧光成像和治疗。利用一系列表征手段，对纳米粒子进行光学性能分析和结构表征。以盐酸阿霉素(DOX)为药物模型，对制备的复合荧光纳米药物载体进行药物吸附性和释放性的考察；通过MTT和细胞成像实验考察纳米载体的生物安全性和成像效果。药物吸附性测试表明，该复合纳米载体的载药率为23.08%，包封率为76.94%。体外药物释放实验表明，该复合纳米载体对pH具有敏感响应。MTT的结果表明制备的复合纳米载体具有较小的细胞毒性，适用于生物应用，同时也证明了负载了DOX的复合纳米药物载体具有杀死肿瘤细胞的作用。肿瘤细胞成像实验表明复合纳米载体在肿瘤细胞中达到良好的荧光成像效果，可用于体外生物成像领域。本研究成功制备了基于荧光Si NPs的pH响应型复合纳米药物载体，以抗肿瘤药物DOX为药物模型，该复合纳米药物载体具有良好的药物装载率和包封率，并且可以实现对肿瘤细胞荧光实时成像和治疗作用。
Silicon nanoparticles (Si NPs) prepared by a one-pot hydrothermal method were used as fluorescent carriers, while mesoporous silica nanoparticles (MSNs) were used as drug carriers, pH responsive composite fluorescent nanocarriers (Si NPs-MSNs) were prepared and used for fluorescence imaging and treatment of tumor cells. Using a series of characterization methods to analyze the optical properties and structural characterization of nanomaterials. Using doxorubicin hydrochloride (DOX) as a drug model, the drug adsorption and release properties of the prepared composite fluorescent nano drug carrier were investigated; Investigating the biosafety and imaging effectiveness of nanocarriers through MTT and cell imaging experiments. The drug adsorption test showed that the drug loading rate of the composite nanocarrier was 23.08%, and the encapsulation efficiency was 76.94%. In vitro drug release experiments showed that the composite nanocarrier has a sensitive response to pH. The MTT results indicate that the prepared composite nanocarrier has low cytotoxicity and is suitable for biological applications. It also proves that the composite nanocarrier loaded with DOX has the ability to kill tumor cells. Tumor cell imaging experiments have shown that composite nanocarriers can exhibit good fluorescence imaging effects in tumor cells and can be used in the field of in vitro biological imaging. This study successfully prepared a pH responsive composite nano drug carrier based on fluorescent Si NPs, using the anti-tumor drug DOX as the drug model. The composite nano drug carrier has good drug loading and encapsulation efficiency, and can achieve real-time fluorescence imaging and therapeutic effects on tumor cells.
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