Seed dormancy and seed-borne fungi are main biological constraints to the domestication of edible non-timber forest products such as Tetrapleuratetraptera fruits. This study aimed to evaluate the effect of thermal and mechanical scarification on seed dormancy and the efficacy of three plant aqueous extracts (Cymbopogon citratus, Callistemomviminalis, Tephrosia vogelii) against seed-borne diseases and seedlings vigour of T. tetraptera. Treatments consisted of soaking the seeds in water at 80？C for 2 and 4 hours, scarification of seeds with abrasive paper at 1 mm and 2 mm depths. The antifungal activity of plant extracts was evaluated both in vitro (by the poising method on agar medium) and in vivo at 2.5, 5.0 and 7.5 mg/mL. Momtaz (Imidacloprid 250 g/kg + Thiram 200 g/kg) was used as a positive control. Dormancy was lifted by scarification at 2 mm depth; this treatment had the highest germination percentage (92.33%) and the lowest infection rate (20.67%). The more frequent seed-borne fungi isolated belong to Aspergillus sp. (18.43% - 21.78%). The pathogenicity test was positive with Alternariaalternata, A. fumigatus and Cercospora sp. T. vogelii extract totally inhibited the growth of the pathogenic fungi at all the concentrations tested. Seed infection obtained with T. vogelii (4.12%) extracts at 7.5 mg/mL was significantly similar to Momtaz (3.33%). The extract of C. viminalis had the highest vigour index (674.42) at 7.5 mg/mL. Mechanical scarification using abrasive paper and seed treatment with plant extracts of T. vogelii could be used in the domestication process of the species.
References
[1]
Betti, J.L., Ngankoué, C.M., Dibong, S.D. and Singa, A.E. (2017) Etude ethnobotanique des plantes alimentaires spontanées vendues dans les marchés de Yaoundé, Cameroun. International Journal of Biological and Chemical Sciences, 10, 1678-1693. https://doi.org/10.4314/ijbcs.v10i4.19
[2]
Manfo, D. (2018) Practices and Challenges of Agroforestry in the Forest-Savannah Contact Zone: The Case of Obala in the Center Region of Cameroon. Revue Scientifique et Technique Foret et Environnement du Bassin du Congo, 11, 66-78.
[3]
Muyambo, M.-A., Kimoni, K. and Furaha, A.S. (2017) Forest Products and Rural Household Livelihood Strategies in the Kisangani Region. Tropenbos DR Congo.
[4]
Awono, A., Eba’a Atyi, R., Foundjem-Tita, D. and Levang, P. (2016) Vegetal Non-Timber Forest Products in Cameroon, Contribution to the National Economy. International Forestry Review, 18, 66-77. https://doi.org/10.1505/146554816819683708
[5]
Eyog, M.O., Ndoye, O., Kengue, J. and Awono, A. (2006) The Edible Forest Fruits of Cameroon. IPGRI/CIRAD.
[6]
Organisation de l’Unité Africaine/Centre Scientifique et Technique de Recherche (1996) Traditional Medicine and Pharmacopoeia: Contribution to Ethnobotanical and floristic Studies in Cameroon. OAU/STRC.
[7]
Walter, S. (2001) Non-Wood Forest Products in Africa: A Regional and National Overview. EC-FAO Partnership Program FAO Working Paper, FOPW/01.
[8]
Sunderland, T.C.H. and Obama, C. (2000) Preliminary Market Study on Non-Timber Forest Products in Equatorial Guinea. FAO, 223-233.
[9]
Tabuna, H. (2000) The European Market for Non-Timber Forest Products from Central Africa. FAO, 267-280.
[10]
Ndoye, O., Ruiz-Perez, M. and Eyebe, A. (2000) The Influence of the Marketing of Non-Timber Forest Products on the Degradation of Resources in Central Africa: The Role of Research in the Balance between the Well-Being Populations and the Preservation of Forests. FAO, 193-217.
[11]
Alexandre, D.Y. (1978) Le rôle disséminateur des éléphants en forêt de Taï, Côte d’ivoire. La Terre et La Vie, Revue d’Histoire naturelle, 32, 47-72. https://doi.org/10.3406/revec.1978.4994
[12]
Wakawa, L.D. and Akinyele, A.O. (2016) Effects of Pre-Treatment on the Germination Response of Old Seed of Tetrapleura tetraptera (Schum. and Thonn.) Taub. Journal of Forest Science and Environment, 1, 81-86.
[13]
David, I.G., Matache, M.L., Tudorache, A., Chisamera, G., Rozylowicz, L. and Radu, G.L. (2012) Food Chain Biomagnification of Heavy Metals in Samples from the Lower Prut Floodplain Natural Park. Environmental Engineering and Management Journal, 11, 69-73. https://doi.org/10.30638/eemj.2012.010
[14]
Kausik, M. and Sayan, J. (2015) A Review on the Effects of Heavy Metals on the Aquatic Animal of Three Different Districts of West Bengal. Journal of Global Biosciences, 4, 2504-2512.
[15]
Isman, M.B. (2000) Plant Essential Oils for Pest and Disease Management. Crop Protection, 19, 603-608. https://doi.org/10.1016/s0261-2194(00)00079-x
[16]
CABI (2011) Natural Products in Plant Pest Management. In: Dubey, N.K., Ed., Centre for Advanced Studies in Botany, Banaras Hindu University, 21-41.
[17]
Ahoton, L.E. and Quenum, F. (2012) Floral Biology and Hybridization Potential of Nine Accessions of Physic Nut (Jatropha curcas L.) Originating from Three Continents. Tropicultura, 30, 193-198.
[18]
Djeugap, F.J., Bernier, L., Dostaler, D., Fontem, D.A. and Avana, M.L. (2014) Germination Constraints on Ricinodendronheudelotii in Cameroon. Seed Technology, 36, 25-36.
[19]
Djeugap, F.J., Tsopmbeng, N.G., Keuete, K.E., Yaouba, A. and Serferbe, S. (2015) Isolation and Identification of Fungi Associated with Avocado Fruits from Local Markets of the West Region of Cameroon. International Journal of Agriculture and Biology, 4, 64-68.
[20]
Djeugap, F.J., Nzuta, C., Temgoua, L.F., Kenmogne, G. and Tekam, P.M. (2017) Champignons pathogènes associés aux semences de Pericopsis elata et effet des substrats sur la germination, la croissance et l’infection des plantules au Cameroun. Revue Scientifique et Technique Forêt et Environnement Du Bassin Du Congo, 8, 19-27.
[21]
Iqbal, N. and Saeed, S. (2012) Isolation of Mango Quick Decline Fungi from Mango bark Beetle, Hypocryphalusmangiferae (Coleoptera: Scolytidae). Journal of Animal and Plant Sciences, 22, 644-648.
[22]
Champion, R. (1997) Identifier les champignons transmis par les semences. Techniques et pratiques. INRA, Editions Quae.
[23]
Imathiu, S., Edwards, S., Ray, R. and Back, M. (2014) Review Article: Artificial Inoculum and Inoculation Techniques Commonly Used in the Investigation of Fusarium Head Blight in Cereals. Acta Phytopathologica et EntomologicaHungarica, 49, 129-139. https://doi.org/10.1556/aphyt.49.2014.2.1
[24]
Mathur, S.B. and Kongsda, O. (2003) Methods for Detecting Fungi. Common Laboratory Seed Health Testing. International Seed Testing Association.
[25]
Falleh, H., Ksouri, R., Chaieb, K., Karray-Bouraoui, N., Trabelsi, N., Boulaaba, M., et al. (2008) Phenolic Composition of Cynara cardunculus L. Organs, and Their Biological Activities. ComptesRendus. Biologies, 331, 372-379. https://doi.org/10.1016/j.crvi.2008.02.008
[26]
Yaouba, A., Tchikoua, R., Tamsa, A.A. and Mpounze, P. (2017) Antifungal Activities of Some Plants Extract against Cercospora spp Causative Agent of Post-Harvest Fruits Rot. International Journal of Multidisciplinary Research and Development, 4, 200-203.
[27]
Abdul‐Baki, A.A. and Anderson, J.D. (1973) Vigor Determination in Soybean Seed by Multiple Criteria. Crop Science, 13, 630-633. https://doi.org/10.2135/cropsci1973.0011183x001300060013x
[28]
Nkongmeneck, B.A., Nwaga, D., Ndemmeze, A. and Hallé, F. (1996) Caractéristiques et capacités de germination des graines de Tetrapleura tetraptera (Shum. & Thonn.) Taub. (Mimosaceae) d’après leur situation dans la gousse. Revue d’Écologie (La Terre et La Vie), 51, 117-124. https://doi.org/10.3406/revec.1996.2196
[29]
Pérez-García, F. and González-Benito, M.E. (2006) Seed Germination of Five Helianthemum Species: Effect of Temperature and Presowing Treatments. Journal of Arid Environments, 65, 688-693. https://doi.org/10.1016/j.jaridenv.2005.10.008
[30]
Rao, N.K., Hanson, J., Dalloo, M.E., Ghosh, K., Nowell, D. and Larinde, M. (2006) Manual of Seed Handling in Genbanks. Handbooks for Genbanks No. 8, Biodiversity International.
[31]
Li, X., Baskin, J.M. and Baskin, C.C. (1999) Seed Morphology and Physical Dormancy of Several North American Rhus Species (Anacardiaceae). Seed Science Research, 9, 247-258. https://doi.org/10.1017/s0960258599000252
[32]
Harivel, A., Meunier, Q., Lemmens, R. and Morin, A. (2010) Alternatives to Exotic Species in Uganda-Growth and Cultivation of 85 Indigenous Trees. French Embassy in Uganda-Belgian Technical Cooperation.
[33]
Teketay, D., Granstrom, A. and Tegene, B. (1998) Seed Viability and Germination of Acacia origena, Acacia pilispina and Pterotobiumstellatum in Ethiopia. Tropical Ecology, 39, 225-233.
[34]
Benadjaoud, A., Mihoub, Z. and Mokhtari, A. (2004) Effet de la scarification mécanique sur la germination des graines de Parkinsonia aculeata L. Revue des Sciences et de la Technologie—Synthèse, 12, 45-52.
[35]
Ahoton, L.E., Adjakidje, V. and Akpo, L.E. (2012) Effet des prétraitements des semences sur la germination de Prosopis africana (Guill., Perrot. et Rich.) Taub. International Journal of Biological and Chemical Sciences, 6, 1745-1754.
[36]
Dongmo, G.Z., Djeugap, J.F., Fenohi, N., Kenfack, N.D., Takuete, R. and Teguefouet, P. (2017) Contribution à l’identification des champignons de post-récolte associés aux amandes de Ricinodendronheudelotii et Garcinia kola collectées dans les Hauts Plateaux de l’Ouest Cameroun. International Journal of Biological and Chemical Sciences, 11, 1840-1850. https://doi.org/10.4314/ijbcs.v11i4.33
[37]
Djeugap, J.F., Nzong, C.A., Kyalo, M., et al. (2017) Morphological and Molecular Identification of Pathogenic Fungi of Monodoramyristica Dunal Kernels and Their Response to Different Phytoextracts. International Journal of Advance Agricultural Research, 5, 66-75.
[38]
Erute, M.O. and Oyibo, A.E. (2008) Effects of Three Plant Extracts (Ocimum gratissimum, Acalypha wilkesiana, and Acalypha macrostachya) on Post-Harvest Pathogen of Persea americana. Journal of Medicinal Plants Research, 2, 311-314.
[39]
Samuel, O. and Mu, O. (2015) Fungi Associated with the Spoilage of Post-Harvest Tomato Fruits Sold in Major Markets in Awka, Nigeria. Universal Journal of Microbiology Research, 3, 11-16. https://doi.org/10.13189/ujmr.2015.030201
[40]
Pande, S. and Gupta, A. (2011) Seed Borne Fungi of Oroxylum indicum. Journal of Mycology and Plant Pathology, 41, 312-315.
[41]
Dayan, M.P. (2004) Seed Pathology of Some Selected Forest Tree Species of Bangladesh. Journal of Forest Science, 24, 45-52.
[42]
Duda, G.P., Santos, I.S. and Souza, R.M. (2004) Fungi Associated with Seeds of Forest Species in Brazilian Cerrado. Revista Brasileira de Sementes, 26, 45-50.
[43]
Salem, M.Z.M., Mervat, E.H., Nasser, R.A., Hayssam, M.A., Nader, A.E.S. and Hosam, O.E. (2017) Medicinal and Biological Values of Callistemon viminalis Extracts: History, Current Situation and Prospects. Asian Pacific Journal of Tropical Medicine, 27, 1-9.
[44]
Masete, G.A. (2021) In Vitro Anti-Microbial Activity of Aqueous and Ethanolic Leaf Extracts of Justicia flava and Tephrosia vogelii That Grows in Uganda. Student’s Journal of Health Research Africa, 13, 1-14.
[45]
Galani, Y.J.H., Nguefack, J., Dakole, D.C., Fotio, D., Petchayo, T.S., Fouelefack, F.R., et al. (2013) Antifungal Potential and Phytochemical Analysis of Extracts from Seven Cameroonian Plants against Late Blight Pathogen Phytophthora infestans. International Journal of Current Microbiology and Applied Sciences, 2, 140-154.
[46]
Yaouba, A., Kemmoe, N.R., Nchoutnji, I. and Godswill, N.N. (2019) Antifungal Potential of Eucalyptus saligna and Cupressus lusitanica Extracts against Fungi Responsible for Post-Harvest Green Bean Spoilage in Dschang, West Cameroon. Cameroon Journal of Biological and Biochemical Sciences, 27, 29-37.
[47]
Djeugap, J., Fontem, D. and Tapondjou, A. (2012) Efficacité in Vitro et in Vivo des extraits de plantes contre le mildiou (Phytophthora infestans) de la morelle noire. International Journal of Biological and Chemical Sciences, 5, 2205-2213. https://doi.org/10.4314/ijbcs.v5i6.3
[48]
Helal, G.A., Sarhan, M.M., Abu Shahla, A.N.K. and Abou El-Khair, E.K. (2007) Effects of Cymbopogon citratus L. Essential Oil on the Growth, Morphogenesis and Aflatoxin Production. Journal of Basic Microbiology, 6, 125-131.
[49]
Kpatinvoh, B., Adjou, E.S., Dahouenon-Ahoussi, E., Konfo, T.R.C., Atrevi, B., Soumanou, M.M., et al. (2017) Efficacy of Essential Oils of Three Aromatic Plants against Cowpea Spoilage Mycoflora (Vigna unguiculata L., Walp) Collected from Retail Outlets in Southern Benin. Journal of Applied Biosciences, 109, 10680-10687. https://doi.org/10.4314/jab.v109i1.12
[50]
Yoshimura, H., Sawai, Y., Tamotsu, S. and Sakai, A. (2011) 1,8-Cineole Inhibits both Proliferation and Elongation of BY-2 Cultured Tobacco Cells. Journal of Chemical Ecology, 37, 320-328. https://doi.org/10.1007/s10886-011-9919-2
