|
|
Botanical Research 2025
WOX5在拟南芥根尖的调控功能：组织与器官之间的落差
|
Abstract:
WOX5是拟南芥根尖干细胞调控网络中的一个关键因子，正常时空表达时主要在静止中心和柱干细胞中发挥作用，在细胞与组织层面具有促进干细胞特性维持和抑制细胞分化的功能，但WOX5功能完全缺失的突变体在器官与个体水平没有明显的表型，弄清引起WOX5在细胞组织和器官个体之间功能差异的原因可能会刷新我们目前有关根干细胞调控的认识。
WOX5 is a key factor in the regulatory network of root tip stem cells in Arabidopsis. Under normal spatiotemporal expression, it primarily functions in the quiescent center (QC) and columella stem cells, promoting stem cell identity maintenance and suppressing cell differentiation at the cellular and tissue levels. However, WOX5 loss-of-function mutants show no obvious phenotype at the organ or whole-plant level. Understanding the mechanisms behind this functional disparity in WOX5 between cellular tissue and organ individual levels may reshape our current understanding of root stem cell regulation.
| [1] | Dolan, L., Janmaat, K., Willemsen, V., Linstead, P., Poethig, S., Roberts, K., et al. (1993) Cellular Organisation of the Arabidopsis thaliana Root. Development, 119, 71-84. https://doi.org/10.1242/dev.119.1.71 |
| [2] | Bennett, T. and Scheres, B. (2010) Root Development—Two Meristems for the Price of One? In: Current Topics in Developmental Biology, Elsevier, 67-102. https://doi.org/10.1016/s0070-2153(10)91003-x |
| [3] | Eljebbawi, A., Dolata, A., Strotmann, V.I. and Stahl, Y. (2024) Stem Cell Quiescence and Dormancy in Plant Meristems. Journal of Experimental Botany, 75, 6022-6036. https://doi.org/10.1093/jxb/erae201 |
| [4] | Dubrovsky, J.G. and Ivanov, V.B. (2021) The Quiescent Centre of the Root Apical Meristem: Conceptual Developments from Clowes to Modern Times. Journal of Experimental Botany, 72, 6687-6707. https://doi.org/10.1093/jxb/erab305 |
| [5] | Sarkar, A.K., Luijten, M., Miyashima, S., Lenhard, M., Hashimoto, T., Nakajima, K., et al. (2007) Conserved Factors Regulate Signalling in Arabidopsis thaliana Shoot and Root Stem Cell Organizers. Nature, 446, 811-814. https://doi.org/10.1038/nature05703 |
| [6] | Savina, M.S., Pasternak, T., Omelyanchuk, N.A., Novikova, D.D., Palme, K., Mironova, V.V., et al. (2020) Cell Dynamics in WOX5-Overexpressing Root Tips: The Impact of Local Auxin Biosynthesis. Frontiers in Plant Science, 11, Article 560169. https://doi.org/10.3389/fpls.2020.560169 |
| [7] | Sharma, M., Friedrich, T., Oluoch, P., Zhang, N., Peruzzo, F., Jha, V., et al. (2024) A Coherent Feed-Forward Loop in the Arabidopsis Root Stem Cell Organizer Regulates Auxin Biosynthesis and Columella Stem Cell Maintenance. Nature Plants, 10, 1737-1748. https://doi.org/10.1038/s41477-024-01810-z |
| [8] | Ding, Z. and Friml, J. (2010) Auxin Regulates Distal Stem Cell Differentiation in Arabidopsis Roots. Proceedings of the National Academy of Sciences, 107, 12046-12051. https://doi.org/10.1073/pnas.1000672107 |
| [9] | Vilarrasa-Blasi, J., González-García, M., Frigola, D., Fàbregas, N., Alexiou, K.G., López-Bigas, N., et al. (2014) Regulation of Plant Stem Cell Quiescence by a Brassinosteroid Signaling Module. Developmental Cell, 30, 36-47. https://doi.org/10.1016/j.devcel.2014.05.020 |
| [10] | Betegón‐Putze, I., Mercadal, J., Bosch, N., Planas‐Riverola, A., Marquès‐Bueno, M., Vilarrasa‐Blasi, J., et al. (2021) Precise Transcriptional Control of Cellular Quiescence by BRAVO/WOX5 Complex in Arabidopsis Roots. Molecular Systems Biology, 17, e9864. https://doi.org/10.15252/msb.20209864 |
| [11] | Zhang, W., Swarup, R., Bennett, M., Schaller, G.E. and Kieber, J.J. (2013) Cytokinin Induces Cell Division in the Quiescent Center of the Arabidopsis Root Apical Meristem. Current Biology, 23, 1979-1989. https://doi.org/10.1016/j.cub.2013.08.008 |
| [12] | Zhang, W., To, J.P.C., Cheng, C., Eric Schaller, G. and Kieber, J.J. (2011) Type‐A Response Regulators Are Required for Proper Root Apical Meristem Function through Post‐Transcriptional Regulation of PIN Auxin Efflux Carriers. The Plant Journal, 68, 1-10. https://doi.org/10.1111/j.1365-313x.2011.04668.x |
| [13] | Aida, M., Beis, D., Heidstra, R., Willemsen, V., Blilou, I., Galinha, C., et al. (2004) The Plethora Genes Mediate Patterning of the Arabidopsis Root Stem Cell Niche. Cell, 119, 109-120. https://doi.org/10.1016/j.cell.2004.09.018 |
| [14] | Pi, L., Aichinger, E., van der Graaff, E., Llavata-Peris, C.I., Weijers, D., Hennig, L., et al. (2015) Organizer-Derived WOX5 Signal Maintains Root Columella Stem Cells through Chromatin-Mediated Repression of CDF4 Expression. Developmental Cell, 33, 576-588. https://doi.org/10.1016/j.devcel.2015.04.024 |
| [15] | Leibfried, A., To, J.P.C., Busch, W., Stehling, S., Kehle, A., Demar, M., et al. (2005) WUSCHEL Controls Meristem Function by Direct Regulation of Cytokinin-Inducible Response Regulators. Nature, 438, 1172-1175. https://doi.org/10.1038/nature04270 |
| [16] | 王甜, 严欢欢, 陈丽华, 张汉马. 位置效应对拟南芥根冠柱细胞的影响[J]. 植物学研究, 2020, 9(3): 262-267. |
