This trial aims to find out the best storage temperature for various seed types to improve viability during storage. More recently, a large quantity of economically important crop seeds is lost within a short period due to poor storage. In most circumstances, this can increase seed costs, reduce seed quantity and quality and may lead to seed shortages during peak seasons. As such, a project was carried out at NAREI Seed Laboratory to ascertain the best storage condition for different types of seed. This experiment was laid out in a Strip Plot Design with two factors: temperature with four levels (28.7?C, 7.8?C, 5.2?C and ?14?C) and seed types with seven levels (corn, red beans, mung beans, ochro, boulanger, watermelon, and cucumber) and replicated four times. One kilogram of various seed types was then placed in different storage conditions. Germination test was conducted at intervals of 3, 6, 9 and 12 months after storage. The interaction of seed types and storage temperature was significant, whereby red bean and mung bean seeds recorded significantly greater germination percent at ?14?C, while corn, cucumber, watermelon and ochro seeds prefer to be stored at 5.2?C due to significantly higher percent germination. On the other hand, cucumber and boulanger seeds recorded greater percent germination at 7.8?C. The interaction of storage temperature and storage time was highly significant where seeds stored at 5.2 and 7.8 degrees celsius recorded greater germination percent at 3, 6, 9 and 12 months after storage. Most seeds should be stored at 5.2 degrees celsius to maintain seed viability during storage. By utilizing this temperature, more seeds can be available to farmers in the desired quality and quantity. Regardless of storage temperature, all seed types experienced a considerable decline in percentage germination after 12 months. Therefore, these temperatures are only suitable for short-term seed storage.
References
[1]
Hezewijk, M.J.V., Beem, A.P.V., Verkleij, J.A.C. and Pieterse, A.H. (1993) Germination of orobanche Crenata Seeds, as Influenced by Conditioning Temperature and Period. Canadian Journal of Botany, 71, 786-792. https://doi.org/10.1139/b93-090
[2]
Schmidt, L. (2002) Guide to Handling of Tropical and Subtropical Forest Seeds. DANIDA Forest Seed Centre.
[3]
Nasreen, S. (2000) Effects of Storage Temperature, Storage Period and Seed Moisture Content on Seed Viability of Mash Bean (Vigna mungo). Pakistan Journal of Biological Sciences, 3, 513-514. https://doi.org/10.3923/pjbs.2000.513.514
[4]
Müller, E., Cooper, E.J. and Alsos, I.G. (2011) Germinability of Arctic Plants Is High in Perceived Optimal Conditions but Low in the Field. Botany, 89, 337-348. https://doi.org/10.1139/b11-022
[5]
Bonner, F.T. (1990) Storage of Seeds: Potential and Limitations for Germplasm Conservation. Forest Ecology and Management, 35, 35-43. https://doi.org/10.1016/0378-1127(90)90230-9
[6]
Yang, Q.H., et al. (2005) Seed Germination Eco-Physiology of Mikania Micrantha HBK. Botanical Bulletin of Academia Sinica, 46, 293-299.
[7]
Onyekwelu, J.C. and Fayose, O.J. (2007) Effect of Storage Methods on the Germination and Proximate Composition of Treculia africana Seeds. Proceedings of the International Conference on Agricultural Research for Development, Tropentag, 9-11 October 2007, 1-4.
[8]
Pradhan, B.K. and Badola, H.K. (2008) Seed Germination Response of Populations of Swertia chirayita [(Roxb. Ex-Fleming) H. Karst] Following Periodical Storage. Seed Technology, 30, 63-69.
[9]
Christensen, C.M. (1972) Microflora and Seed Deterioration. In: Roberts, E.H., Ed., Viability of Seeds, Springer, 59-93. https://doi.org/10.1007/978-94-009-5685-8_3
[10]
Roberts, E.H. (1973) Predicting the Storage Life of Seeds. Seed Science and Technology, 1, 499-514.
[11]
Butola, J.S. and Badola, H.K. (2004) Effect of Pre-Sowing Treatment on Seed Germination and Seedling Vigour in Angelica Glauca, a Threatened Medicinal Herb. Current Science, 87, 796-799.
[12]
Butola, J.S. and Badola, H.K. (2004) Seed Germination Improvement Using Chemicals in Heracleum candicans Wall., a Threatened Medicinal Herb of Himalaya. Indian Forester, 130, 565-572.
[13]
Chen, S.Y., Kuo, S.R. and Chien, C.T. (2007) Storage Behaviour of Seeds of Cinnamomumosmophloeum and Neolitsea aciculata Var. Variabillima (Lauraceae). Seed Science and Technology, 35, 237-243. https://doi.org/10.15258/sst.2007.35.1.22
[14]
Bayer (2021) Agronomic Spotlight, Vegetable Seed Storage and Handling. Bayer Vegetable Canada. https://www.vegetables.bayer.com/ca/en-ca/resources/agronomic-spotlights/vegetable-seed-storage-and-handling.html
[15]
Akamine, E.K. (1943) The Effect of Temperature and Humidity on Viability of Stored Seeds in Hawaii. Hawaii Agricultural Experiment Station, Bulletin No. 90, 23 p.
[16]
Genes, F. and Nyomora, A. (2018) Effect of Storage Time and Temperature on Germination Ability of Escoecaria bussei. Tanzania Journal of Science, 44, 123-133.
[17]
Pradhan, B.K. and Badola, H.K. (2012) Effect of Storage Conditions and Storage Periods on Seed Germination in Eleven Populations of Swertia chirayita: A Critically Endangered Medicinal Herb in Himalaya. The Scientific World Journal, 2012, Article ID: 128105. https://doi.org/10.1100/2012/128105
[18]
Yoshinaga, A.Y. (2010) Guidelines for Successful Seed Storage. A Center for Conservation Research and Training. Gilmore Honolulu, 1-7.
[19]
ISTA (2015) Chapter 5: The Germination Test. International Rules for Seed Testing, 2015, 5-1-5-56. https://doi.org/10.15258/istarules.2015.05
[20]
Demisie, W., Mengistu, T. and Kindie, A. (2019) Effects of Different Soil Media Mixtures on Seedling Growth Performance of Tomato (Lycopersican esculentam L.) in Jigjiga District, Ethiopia. International Journal of Advanced Scientific Research & Development (IJASRD), 6, 1-7. https://ssrn.com/abstract=3469085
[21]
TNADU (2015) Seed Science and Technology. https://agritech.tnau.ac.in/seed_certification/seed%20Storage_Storage%20factors.html
[22]
Šimić, B., Vratarić, M., Sudarić, A., Krizmanić, M. and Andrić, L. (2005) Effect of Storage Longevity under Different Storage Conditions on Seed Vigor and Oil Content in Maize, Soybean and Sunflower. Poljoprivreda, 11, 12-17.
[23]
Walters, C., Wheeler, L.M. and Grotenhuis, J.M. (2005) Longevity of Seeds Stored in a Genebank: Species Characteristics. Seed Science Research, 15, 1-20. https://doi.org/10.1079/ssr2004195
[24]
Rajjou, L. and Debeaujon, I. (2008) Seed Longevity: Survival and Maintenance of High Germination Ability of Dry Seeds. Comptes Rendus. Biologies, 331, 796-805. https://doi.org/10.1016/j.crvi.2008.07.021
[25]
Crocker, W. (1930) Harvesting, Storage and Stratification of Seeds in Relation to Nursery Practice. Boyce Thompson Institute for Plant Research.