缓释肥对不同基因型丹参生理特性的影响
Effect of Slow-Release Fertilizer on Physiological Characteristics of Different Genotypes of Salvia miltiorrhiza

为了明确缓释肥对丹参生理特性的影响，本研究采用盆栽实验，研究不同缓释肥用量0 kg/hm² (N1)、120 kg/hm² (N2)、240 kg/hm² (N3)、360 kg/hm² (N4)、480 kg/hm² (N5)对三种基因型的丹参幼苗SY-JM-L4 (A1)，DY-SD-8 (A2)，YY-YM-X1 (A3)的株高、叶绿素含量、可溶性糖、可溶性蛋白和抗氧化酶活性等指标的影响。研究结果表明，施用缓释肥显著提高丹参的株高，其中A1和A2基因型的株高在N4处理下达到最大值，而A3基因型的株高在N3处理下达到最大值，分别比对照处理提高了42.4%、75.4%和70.7%。3种基因型丹参总叶绿素含量均在N3处理下最高，分别比对照处理提高17.9%、13.5%和11.3%。3种基因型丹参的可溶性糖、可溶性蛋白含量均在N3处理下达到最大值。A1和A2基因型丹参的POD活性在N5施肥水平下达到最大值，A1和A2丹参的SOD活性最高在N3施肥条件下最高。综合考虑，缓释肥施用量为N3 (240 kg/hm²)时适合3种基因型丹参的生长。
To elucidate the effect of slow-release fertilizer (SFR) on the physiological characteristics of Salvia miltiorrhiza (S. miltiorrhiza), the present study was conducted in a pot experiment to investigate the effects of different slow-release fertilizer dosages of 0 kg/hm² (N1), 120 kg/hm² (N2), 240 kg/hm² (N3), 360 kg/hm² (N4), 480 kg/hm² (N5) on the plant height, chlorophyll content, soluble sugars, soluble proteins and antioxidant enzymes activities of three genotypes S. miltiorrhiza seedlings (SY-JM-L4 (A1), DY-SD-8 (A2) and YY-YM-X1 (A3). The results showed that the SFR significantly augmented the plant height of the three genotypes of S. miltiorrhiza. The maximum plant height of A1 and A2 was observed at N4 treatment, while A3 was observed at N3 treatment, which was 42.43%, 75.37%, and 70.72% higher than that of the control treatments. The total chlorophyll content in three genotypes of S. miltiorrhiza was found to be the highest at N3 treatment, which was 17.94%, 13.47% and 11.30% higher than the control treatment, respectively. The soluble sugars and soluble protein contents of three genotypes of S. miltiorrhiza attained the maximum values at N3 treatment. Furthermore, the POD activity of the A1 and A2 genotypes of S. miltiorrhiza reached the maximum at N4 treatment, and the SOD activity in A1 and A2 reached its maximum at N3 treatment. Consequently, it can be concluded that the SRF application rate of N3 (240 kg/hm²) was determined to be the optimal for the growth of the three genotypes of S. miltiorrhiza.