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基于网络药理学的刺五加注射液治疗冠心病心绞痛作用机制
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Abstract:
目的：初步探索刺五加注射液治疗冠心病心绞痛的作用机制。方法：通过检索ECTM数据库和文献收集刺五加注射液的化合物成分，后利用PubChem、SwissADME、SwissTargetPrediction数据库获得刺五加注射液的化合物靶点数据集。通过TTD、DisGeNET、GeneCards三大数据库获得冠心病心绞痛相关靶点，与刺五加注射液靶点合并去重后取交集并绘制Venny图。运用Cyoscape 3.9.1软件筛选核心靶点与构建疾病–交集靶点–化合物网络。利用Metascape平台进行KEGG通路和GO功能富集分析，并利用微生信平台绘制点线图和矩阵点图。利用Autodock tools1.5.7、Autodock vina1.1.2对核心靶点及对应成分进行分子对接，并运用Pymol 2.5软件进行视化处理。结果：刺五加注射液主要作用于TNF、IL6、NFKB1、HIF-1A等核心靶点，调节IL-17、脂质与动脉粥样硬化等信号通路从而发挥治疗冠心病心绞痛的作用。
Objective: To explore the mechanism of action of Eleuthero in the treatment of coronary heart disease angina. Methods: The compound components of Eleuthero were collected by searching the ECTM database and literature, and then the compound target dataset of Eleuthero was obtained by using PubChem, SwissADME and SwissTargetPrediction databases. The angina related targets of coronary heart disease were obtained through the three databases of TTD, DisGeNET and GeneCards, and the targets of Eleuthero were combined and deduplicated, and the Venny diagram was plotted. Cyoscape 3.9.1 software was used to screen core targets and construct disease-intersection target-compound networks. The Metascape platform was used for KEGG pathway and GO function enrichment analysis, and the micro-biotech platform was used to draw dot line diagrams and matrix dot diagrams. Autodock tools 1.5.7 and Autodock vina 1.1.2 were used to molecularly dock the core targets and corresponding components, and Pymol 2.5 software was used for visualization processing. Results: Eleuthero mainly acts on core targets such as TNF, IL6, NFKB1, HIF-1A, etc., and regulates IL-17, lipid and atherosclerosis signaling pathways, thereby playing a role in the treatment of coronary heart disease angina.
| [1] | Nair, T,. Jadhav, U.M., Kumar, K.P.S., et al. (2021) Consensus Statement on Holistic Management of Angina. Journal of the Association of Physicians of India, 69, 11-12. |
| [2] | 魏鹏路, 邢文龙, 李享, 等. 基于网状Meta分析的中药治疗不稳定性心绞痛的有效性及安全性[J]. 中国实验方剂学杂志, 2022, 28(5): 197-210. |
| [3] | 沙靖昱, 刘峘, 谢雁鸣, 等. 基于真实世界刺五加注射液5904例心血管病患者的临床用药特征[J]. 中国中药杂志, 2020, 45(15): 3525-3532. |
| [4] | 秦腾腾, 张平, 刘峘, 等. 基于真实世界中刺五加注射液应用于心血管病的复杂网络分析[J]. 世界科学技术-中医药现代化, 2021, 23(2): 526-535. |
| [5] | 成冯镜茗, 谢雁鸣, 王连心, 等. 真实世界刺五加注射液治疗2046例冠心病患者的临床用药特征分析[J]. 中国中医基础医学杂志, 2021, 27(10): 1588-1594. |
| [6] | 余文怡, 伍慧敏, 郭秀洁, 等. 基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱的刺五加注射液化学成分分析[J]. 色谱, 2023, 41(3): 207-223. |
| [7] | Abdulfattah, S.Y. and Samawi, F.T. (2023) Estimating the Role of Single-Nucleotide Polymorphism (RS1800629)-308 G/A of TNF-α Gene as Genetic Marker Associated with Angina Pectoris in a Sample of Iraqi Patients. Journal of Genetic Engineering and Biotechnology, 21, 2. https://doi.org/10.1186/s43141-022-00454-w |
| [8] | 李琴, 刘丽军, 信栓力, 等. 心绞痛患者血浆白细胞介素-6和肿瘤坏死因子-α的变化及临床意义[J]. 中国循证心血管医学杂志, 2013, 5(2): 161-162. |
| [9] | Sepehri, Z.S., Masoomi, M., Ruzbehi, F., Kiani, Z., Nasiri, A.A., Kohan, F., et al. (2018) Comparison of Serum Levels of IL-6, IL-8, TGF-β and TNF-α in Coronary Artery Diseases, Stable Angina and Participants with Normal Coronary Artery. Cellular and Molecular Biology, 64, 1-6. https://doi.org/10.14715/cmb/2018.64.5.1 |
| [10] | 李卓伦, 杨彦涛, 孙志, 等. 基于UHPLC-Q-Orbitrap和网络药理学的灯盏生脉胶囊治疗心绞痛的机制研究[J]. 中草药, 2021, 52(12): 3501-3513. |
| [11] | Luo, J., Liu, F., Zhang, T., Tian, T., Luo, F., Li, X., et al. (2022) Association of NFKB1 Gene Rs28362491 Mutation with the Occurrence of Major Adverse Cardiovascular Events. BMC Cardiovascular Disorders, 22, Article No. 313. https://doi.org/10.1186/s12872-022-02755-x |
| [12] | 苑明川, 王莉, 王贺, 等. HIF-1α在动脉粥样硬化中的作用研究进展[J]. 中国动脉硬化杂志, 2023, 31(9): 815-820. |
| [13] | Kang, J., Kim, D., Rhee, J., Seo, J., Park, I., Kim, J., et al. (2023) Baf155 Regulates Skeletal Muscle Metabolism via HIF-1a Signaling. PLOS Biology, 21, e3002192. https://doi.org/10.1371/journal.pbio.3002192 |
| [14] | Khan, S.S., Shah, S.J., Strande, J.L., Baldridge, A.S., Flevaris, P., Puckelwartz, M.J., et al. (2021) Identification of Cardiac Fibrosis in Young Adults with a Homozygous Frameshift Variant in SERPINE1. JAMA Cardiology, 6, 841-846. https://doi.org/10.1001/jamacardio.2020.6909 |
| [15] | and Alternative Medicine, E.C. (2023) Retracted: SERPINE1 Gene Is a Reliable Molecular Marker for the Early Diagnosis of Aortic Dissection. Evidence-Based Complementary and Alternative Medicine, 2023, Article ID: 9878327. https://doi.org/10.1155/2023/9878327 |
| [16] | Tian, J., Hu, S., Wang, F., et al. (2015) PPARG, AGTR1, CXCL16 and LGALS2 Polymorphisms Are Correlated with the Risk for Coronary Heart Disease. International Journal of Clinical and Experimental Pathology, 8, 3138-3143. |
| [17] | Maciejewska-Skrendo, A., Pawlik, A., Sawczuk, M., Rać, M., Kusak, A., Safranow, K., et al. (2019) PPARA, PPARD and PPARG Gene Polymorphisms in Patients with Unstable Angina. Gene, 711, Article ID: 143947. https://doi.org/10.1016/j.gene.2019.143947 |
| [18] | Boluri, A., Khazaei, H., Sargolzaei, N., Miri, H.O. and Khazaei, B. (2022) The Comparison of IL-17 Levels in Patients with Unstable Angina before and after Medical Treatment. Human Antibodies, 30, 25-29. https://doi.org/10.3233/hab-210446 |
| [19] | Ma, K., Zhou, H., Zhang, W., Liu, J., Sha, Y., Kong, X., et al. (2024) Study of IL-17 and Intercellular Adhesion Molecule-1 in Conjunctivochalasis Using Correlation Analysis. Cornea, 43, 537-544. https://doi.org/10.1097/ico.0000000000003480 |
| [20] | Zeisbrich, M., Thiel, J. and Venhoff, N. (2024) The IL-17 Pathway as a Target in Giant Cell Arteritis. Frontiers in Immunology, 14, Article 1199059. https://doi.org/10.3389/fimmu.2023.1199059 |
| [21] | Gilani, S.T.A., Khan, D.A., Rauf, A., Haroon, Z.H., Khan, K.A. and Hassan, F.U. (2022) Early Diagnosis of Coronary Artery Disease by Inflammatory Biomarkers of Atherosclerosis in Patients with Angina. Journal of Interferon & Cytokine Research, 42, 493-500. https://doi.org/10.1089/jir.2022.0110 |
| [22] | Hu, G., Jiang, X., Du, S., Zhang, K. and Chen, Z. (2024) miR‐107‐5p Ameliorates Neurological Damage, Oxidative Stress, and Immune Responses in Mice with Alzheimer’s Disease by Suppressing the Toll‐Like Receptor 4 (TLR4)/Nuclear Factor κB (NF‐κB) Pathway. The Kaohsiung Journal of Medical Sciences, 40, 119-130. https://doi.org/10.1002/kjm2.12797 |
| [23] | 林紫薇, 武柳君, 吴晖晖, 等. 基于TNF/NF-κB信号通路探讨枳实薤白桂枝汤减轻心肌梗死大鼠心肌损伤的作用机制[J]. 中国实验方剂学杂志, 2023, 29(18): 8-16. |
| [24] | 李自青, 闫玉清, 张年萍, 等. Toll样受体信号通路与冠心病关系的生物信息学分析[J]. 中国全科医学, 2019, 22(17): 2069-2074. |
