Metabolic disorders such as hyperlipidaemia can be alleviated by drinking water. This study investigated whether lifeceramics (LC)-treated water (LC water) could improve hyperlipidaemia in rats. The LC water was prepared by mixing distilled water with LC particles composed of zeolite and oyster shells. Hyperlipidaemia was induced in rats via gavage with a high-calorie diet (HCD) rich in fat and sugar. The HCD-fed rats were classified into four groups: distilled water-drinking hyperlipidaemia group (hyper LP), low-dose LC water-drinking group (HCD + low LC), high-dose LC water-drinking group (HCD + high LC), and atorvastatin (ATS, a lipid-regulating drug) solution-drinking group (HCD + ATS). Control rats were fed a normal diet and distilled water. All rats were gavaged daily with diet and drinking water, and biochemical indices related to lipid metabolism, oxidative stress, and inflammation were measured after 20 weeks. Notably, higher serum lipid levels, including total cholesterol, triglycerides, and low-density lipoprotein cholesterol, were observed in the hyper-LP rats than in the control rats. However, the LC water-drinking rats exhibited lower serum lipid levels than the hyper-LP rats, as did the HCD + ATS rats. Serum catalase activity and glutathione levels increased and decreased, respectively, in the hyper-LP rats compared with those in the control rats but recovered to around the control levels in the HCD + high LC and HCD + ATS rats. Furthermore, serum tumour necrosis factor-α and adiponectin levels in the hyper-LP rats were increased and decreased, respectively, compared with those in the control rats, whereas their levels recovered to around the control levels following administration of the high or low dose of LC. Therefore, LC water can improve lipid metabolism dysfunction, anti-oxidant capacity, and inflammatory responses in hyperlipidaemic rats.
References
[1]
Gami, A.S., Witt, B.J., Howard, D.E., Erwin, P.J., Gami, L.A., Somers, V.K., et al. (2007) Metabolic Syndrome and Risk of Incident Cardiovascular Events and Death. Journal of the American College of Cardiology, 49, 403-414. https://doi.org/10.1016/j.jacc.2006.09.032
[2]
Catapano, A.L., Maggi, F.M. and Tragni, E. (2000) Low Density Lipoprotein Oxidation, Antioxidants, and Atherosclerosis. Current Opinion in Cardiology, 15, 355-363. https://doi.org/10.1097/00001573-200009000-00008
[3]
Maury, E. and Brichard, S.M. (2010) Adipokine Dysregulation, Adipose Tissue Inflammation and Metabolic Syndrome. Molecular and Cellular Endocrinology, 314, 1-16. https://doi.org/10.1016/j.mce.2009.07.031
[4]
Kanoski, S.E. and Davidson, T.L. (2011) Western Diet Consumption and Cognitive Impairment: Links to Hippocampal Dysfunction and Obesity. Physiology & Behavior, 103, 59-68. https://doi.org/10.1016/j.physbeh.2010.12.003
[5]
Jiang, C.J. and Yuan, Q. (2016) Side Effects and Clinical Observation of Statins. Chinese Journal of Practical Medicine, 11, 193-194.
[6]
Lahera, V., Goicoechea, M., Garcia de Vinuesa, S., Miana, M., de las Heras, N., Cachofeiro, V., et al. (2007) Endothelial Dysfunction, Oxidative Stress and Inflammation in Atherosclerosis: Beneficial Effects of Statins. Current Medicinal Chemistry, 14, 243-248. https://doi.org/10.2174/092986707779313381
[7]
Karalis, K.P., Giannogonas, P., Kodela, E., Koutmani, Y., Zoumakis, M. and Teli, T. (2009) Mechanisms of Obesity and Related Pathology: Linking Immune Responses to Metabolic Stress. The FEBS Journal, 276, 5747-5754. https://doi.org/10.1111/j.1742-4658.2009.07304.x
[8]
Pirola, L. and Ferraz, J.C. (2017) Role of Pro-and Anti-Inflammatory Phenomena in the Physiopathology of Type 2 Diabetes and Obesity. World Journal of Biological Chemistry, 8, 120-128. https://doi.org/10.4331/wjbc.v8.i2.120
[9]
Brestoff, J.R. and Artis, D. (2015) Immune Regulation of Metabolic Homeostasis in Health and Disease. Cell, 161, 146-160. https://doi.org/10.1016/j.cell.2015.02.022
[10]
Lumeng, C.N. and Saltiel, A.R. (2011) Inflammatory Links between Obesity and Metabolic Disease. Journal of Clinical Investigation, 121, 2111-2117. https://doi.org/10.1172/jci57132
[11]
Thompson, P.D., Panza, G., Zaleski, A. and Taylor, B. (2016) Statin-Associated side Effects. Journal of the American College of Cardiology, 67, 2395-2410. https://doi.org/10.1016/j.jacc.2016.02.071
[12]
Vinci, P., Panizon, E., Tosoni, L.M., Cerrato, C., Pellicori, F., Mearelli, F., et al. (2021) Statin-Associated Myopathy: Emphasis on Mechanisms and Targeted Therapy. International Journal of Molecular Sciences, 22, Article 11687. https://doi.org/10.3390/ijms222111687
[13]
Cheon, D.Y. and Jo, S. (2022) Adverse Effects of Statin Therapy and Their Treatment. Cardiovascular Prevention and Pharmacotherapy, 4, 1-6. https://doi.org/10.36011/cpp.2022.4.e4
[14]
Ruscica, M., Ferri, N., Banach, M., Sirtori, C.R. and Corsini, A. (2022) Side Effects of Statins: From Pathophysiology and Epidemiology to Diagnostic and Therapeutic Implications. Cardiovascular Research, 118, 3288-3304. https://doi.org/10.1093/cvr/cvac020
[15]
Abdul-Rahman, T., Bukhari, S.M.A., Herrera, E.C., Awuah, W.A., Lawrence, J., de Andrade, H., et al. (2022) Lipid Lowering Therapy: An Era beyond Statins. Current Problems in Cardiology, 47, Article ID: 101342. https://doi.org/10.1016/j.cpcardiol.2022.101342
[16]
Elseweidy, M.M., Elawady, A.S., Sobh, M.S. and Elnagar, G.M. (2022) Lycopene Ameliorates Hyperlipidemia via Potentiation of Amp-Activated Protein Kinase and Inhibition of Atp-Citrate Lyase in Diabetic Hyperlipidemic Rat Model. Life Sciences, 308, Article ID: 120934. https://doi.org/10.1016/j.lfs.2022.120934
[17]
Dongmo, F., Djantou, E.B., Mahamat, A.H., Dongmo, S.S. and Yanou, N.N. (2024) Effect of Aqueous Extract of Boscia senegalensis on Hyperglycemia, Hyperlipidemia and Oxidative Stress Induced in Rats. Journal of Diabetes Mellitus, 14, 49-68. https://doi.org/10.4236/jdm.2024.141006
[18]
Li, K., Tong, X., Yu, J., Gao, X., Gao, F., Liu, X., et al. (2020) Lifeceramics-Treated Water Reduces Serum Uric Acid Levels and Improves Hemorheological Activity in Hyperuricemic Rats. Biomedical Reports, 13, Article No. 22. https://doi.org/10.3892/br.2020.1329
[19]
Kita, K., Sugaya, S., Tanaka, T., Dong, M., Sato, C. and Suzuki, N. (2013) Effects of Lifeceramics-Treated Water on Resistance to Oxidative Stress in Human Cells. Food & Function, 11, 14-19.
[20]
Kita, K., Fukuyo, M., Kaneda, A. and Suzuki, N. (2021) Antioxidative Effects of Lifeceramics-Treated Water in Cultured Human Cells: Suppression of H2O2-Induced Cell Death, Accumulation of Reactive Oxygen Species, and Changes in Gene Expression. Food & Function, 17, 14-21.
[21]
Liu, X., Guo, S.H., Ma, X., Dong, M. and Suzuki, N. (2018) Protective Effect and Mechanism of Lifeceramics on Chronic Alcoholic Liver Injury in Rats. Chinese Journal of Clinical Rational Drug Use, 11, 40-42.
[22]
I, K., N, M., Y-J, Y. and J-Y, L. (2018) Induce Hyperlipidemia in Rats Using High Fat Diet Investigating Blood Lipid and Histopathology. Journal of Hematology and Blood Disorders, 4, Article 104. https://doi.org/10.15744/2455-7641.4.104
[23]
Rodríguez-Correa, E., González-Pérez, I., Clavel-Pérez, P.I., Contreras-Vargas, Y. and Carvajal, K. (2020) Biochemical and Nutritional Overview of Diet-Induced Metabolic Syndrome Models in Rats: What Is the Best Choice? Nutrition & Diabetes, 10, Article No. 24. https://doi.org/10.1038/s41387-020-0127-4
[24]
Udomkasemsab, A. and Prangthip, P. (2019) High Fat Diet for Induced Dyslipidemia and Cardiac Pathological Alterations in Wistar Rats Compared to Sprague Dawley Rats. Clínica e Investigación en Arteriosclerosis, 31, 56-62. https://doi.org/10.1016/j.arteri.2018.09.004
[25]
Li, K., Tong, X., Yao, W., Wang, X., Suzuki, A. and Suzuki, N. (2017) Study on Hemorheological Properties of Erythrocytes in Asymptomatic Hyperuricemia Rat Model. International Journal of Medical Research and Health Sciences, 6, 1-7.
[26]
Wang, Y., Peng, D., Liu, X., Xie, R. and Li, X. (2017) Validation Research and Regulation Exploration of High Fat-Introduced Hyperlipidemia Model in Rats. Chinese Journal of Comparative Medicine, 6, 5-10.
[27]
Zheng, D., Liang, Q., Zeng, F., Mai, Z., Cai, A., Qiu, R., et al. (2015) Atorvastatin Protects Endothelium by Decreasing Asymmetric Dimethylarginine in Dyslipidemia Rats. Lipids in Health and Disease, 14, Article No. 41. https://doi.org/10.1186/s12944-015-0041-2
[28]
Kubo, K. and Kawai, Y. (2021) Zeolite Improves High-Fat Diet-Induced Hyperglycemia, Hyperlipidemia and Obesity in Mice. Journal of Nutritional Science and Vitaminology, 67, 283-291. https://doi.org/10.3177/jnsv.67.283
[29]
Sato, K. (2002) Do you Drink the Water of Life? Jiyu Kobo Press.
[30]
Tong, X., Dong, M., Kita, K., Zhu, T., Ma, X., Suzuki, A. and Suzuki, N. (2018) Search for Food Materials That Support Healthy Conditions, Based on The Human SOS Response Theory. Chiba Medical Journal, 94, 15-22.
[31]
Fentoğlu, Ö., Kırzıoğlu, F.Y., Bulut, M.T., Kumbul Doğuç, D., Kulaç, E., Önder, C., et al. (2015) Evaluation of Lipid Peroxidation and Oxidative DNA Damage in Patients with Periodontitis and Hyperlipidemia. Journal of Periodontology, 86, 682-688. https://doi.org/10.1902/jop.2015.140561
[32]
Kaviarasan, K., Arjunan, M.M. and Pugalendi, K.V. (2005) Lipid Profile, Oxidant-Antioxidant Status and Glycoprotein Components in Hyperlipidemic Patients with/without Diabetes. Clinica Chimica Acta, 362, 49-56. https://doi.org/10.1016/j.cccn.2005.05.010
[33]
Rivera-Mancía, S., Jiménez-Osorio, A.S., Medina-Campos, O.N., Colín-Ramírez, E., Vallejo, M., Alcántara-Gaspar, A., et al. (2018) Activity of Antioxidant Enzymes and Their Association with Lipid Profile in Mexican People without Cardiovascular Disease: An Analysis of Interactions. International Journal of Environmental Research and Public Health, 15, Article 2687. https://doi.org/10.3390/ijerph15122687
[34]
Yang, R., Shi, Y., Hao, G., Li, W. and Le, G. (2008) Increasing Oxidative Stress with Progressive Hyperlipidemia in Human: Relation between Malondialdehyde and Atherogenic Index. Journal of Clinical Biochemistry and Nutrition, 43, 154-158. https://doi.org/10.3164/jcbn.2008044
[35]
Minhajuddin, M., Beg, Z.H. and Iqbal, J. (2005) Hypolipidemic and Antioxidant Properties of Tocotrienol Rich Fraction Isolated from Rice Bran Oil in Experimentally Induced Hyperlipidemic Rats. Food and Chemical Toxicology, 43, 747-753. https://doi.org/10.1016/j.fct.2005.01.015
Matsubara, M., Maruoka, S. and Katayose, S. (2002) Decreased Plasma Adiponectin Concentrations in Women with Dyslipidemia. The Journal of Clinical Endocrinology & Metabolism, 87, 2764-2769. https://doi.org/10.1210/jcem.87.6.8550
[38]
von Eynatten, M., Hamann, A., Twardella, D., Nawroth, P.P., Brenner, H. and Rothenbacher, D. (2006) Relationship of Adiponectin with Markers of Systemic Inflammation, Atherogenic Dyslipidemia, and Heart Failure in Patients with Coronary Heart Disease. Clinical Chemistry, 52, 853-859. https://doi.org/10.1373/clinchem.2005.060509
[39]
Suzuki, N., Kita, K., Sugaya, S., Tanaka, T., Dong, M. and Tong, X.B. (2015) Health Care Promotion by Lifeceramics. Chiba Researchers Network for Health Care Promotion.
[40]
Suzuki, A., Shiga, T., Sato, K., Shoda, M. and Yamaguchi, J. (2025) Metabolome Analysis in Patients with Heart Failure and Implantable Cardioverter Defibrillators. Heart and Vessels, 40, 86-90. https://doi.org/10.1007/s00380-024-02452-z
