Peanut pastes are the most popular ingredients in Central African cuisine. The processing, preservation, transportation and sales locations can promote the development of bacteria. The objective of this study was to count the germs that are indicators of hygiene and fecal contamination, isolate and identify them in peanut pastes. This is a cross-sectional study with an analytical aim. A total of 320 samples of peanut pastes sold in the city of Bangui were collected by random methods and analyzed in the food microbiology unit of the National Laboratory of Clinical Biology and Public Health. The indicator germs of hygiene and fecal contamination were counted and the other enterobacteria were isolated and identified. The enumeration of mesophilic flora was carried out according to the references and the standard in force NF V 08-051, total coliforms by the standards NF V 08-050, 2010 and NF V 08-060, 2010, fecal coliforms by the standard NF ISO 16649-2, 2001 and enterococci by the horizontal enumeration method according to the standard NFT 90-416. The data were collected on the ODK collection software. The Epi info version 6 software was used for data analysis and processing. The results obtained from this study showed that 96.3% of the peanut pastes were marketed by women. Among the sellers, 6.3% were illiterate; 65.6% had dropped out of primary school; 28.2% had dropped out of high school. The search for critical points using the HACCP method revealed that 3% of the sellers had knowledge of food hygiene, 27% could store peanut pastes well and 1% had an idea of the contamination of peanut pastes by germs. No seller had any knowledge of toxiinfection and food poisoning. The personal hygiene of the vendors showed that 14% had dirty clothes, and 11.88% had lesions on their skin. Note that 61.25% of peanut legs were preserved by humidity, and 1.88% by exposure to the sun. The microbial load of germs indicating hygiene and fecal contamination varied from 56 × 106 CFU/g to 219 × 106 CFU/g for mesophilic aerobic germs; 69 × 106 CFU/g to 71 × 106 CFU/g for total coliforms; 43 × 106 CFU/g to 88 × 106 CFU/g for fecal coliforms and enterococci from 3.65 × 106 CFU/g to 111.8 × 106 CFU/g depending on the place of sale (market). The overall prevalence of contamination was 54% by enterobacteria. Strains of Klebsiella pneumonae were 3.4%, Citrobactere koserii 4.6%, those of Enterobacter sakazakii,
References
[1]
Mejrhit, N., Taouda, H. and Aarab, L. (2015) Evaluation de la qualité hygiénique des Arachides au niveau de la ville de Fès-Maroc [Evaluation of the Hygienic Quality of Peanuts at the City Fes-Morocco]. International Journal of Innovation and Applied Studies, 10, 268-277.
[2]
Reese, G. and Lehrer, S.B. (1999) Food Allergens. In: Frieri, M. and Kettelhut, B., Eds., Food Hypersensitivity and Adverse Reactions: A Practical Guide for Diagnosis and Management, Marcel Dekker, 69-97. https://doi.org/10.1201/9780429083129-4
[3]
Woodroof, J.G. (1983) Peanuts: Production, Processing, Products. 3rd Edition, The Avi Publishing Company.
[4]
Bondom, R.L., Lango-Yaya, E. And Denissio M.M. (2024) Contribution à l’étude des souches fongiques contaminant les pâtes d’arachides à Bangui (République Centra Fricaine). Journal of Biomedical Science and Engineering, 17, 97-106.
[5]
Kendall, C., Esfahani, A., Parker, T., Banach, M., Mitchell, S. and Jenkins, D. (2009) Longer-Term Effects of a Low Glycemic Index Diet on Glycemic Control in Type 2 Diabetes. The FASEB Journal, 23, 563.30-563.30. https://doi.org/10.1096/fasebj.23.1_supplement.563.30
[6]
Essang, T., Magrin, G. and Kadekoy-Tigague, D. (200) Du vivrier au vivrier marchand et à l’intégration sous-régionale: Le cas de la filière arachide. In: Jean-Yves, J., Lamine, S.B. and Christian, F., Eds., Savanes africaines: Des espaces en mutation, des acteurs face à de nouveaux défis, PRASAC, CORAF, 7 p.
[7]
Buisson, Y., Marie, J.L. and Davoust, B. (2008) Ces maladies infectieuses importées par les aliments. Bulletin de la Société de pathologie exotique, 101, 343-347. https://doi.org/10.3185/pathexo3210
[8]
Owhe-Ureghe, U.B., Ekundayo, A.O. Agbonlahor, D.E. Oboh, P.A. and Orhue, P. (1993) Bacteriological Examination of Some Ready-to-Eat Foods Marketed in Ekpoma, Edo State of Nigeria. Nigerian Food Journal, 11, 45-52.
[9]
Diener, U.L., Petrit, R.E. and Cole, R. (1982) Aflatoxins and Other Mycotoxins in Peanuts. In: Pattee, H.E. and Young, C.T., Eds., Peanut science and technology, APRES.
[10]
Kadekoy-Tigague, D. (2000) La filière arachide République centrafricaine. Contraintes et perspectives de développement de la production et de la commercialisation. Document de travail. ICRA/PRASAC, 41.
[11]
Joya, M. (2019) Caractérisation de la biodiversité des souches d’Aspergillus de la section Flavi isolées d’aliments commercialisés au Liban. Approche moléculaire, métabolique et morphologique, 46.
[12]
C.I.R.C. (2002) Centre International de Recherche contre le Cancer. Monograph on the Evaluation of Carcinogenic Risk to Humans, World Health Organization. Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene. Summary of Data Reported and Evaluation, 82, 11-17.
[13]
El Hidja, D. (2013) méThodes officielles d’analyses relatives à la microbiologie des aliments (méthodes horizontales). Journal officiel de la republique algerienne, 3-28.
NF EN ISO 4833-1 (2006) Microbiologie des aliments-Méthode horizontale pour le dénombrement des micro-organismesPartie 1: Comptage des colonies à 30°C par la technique d’ensemencement en profondeur, 20.
[16]
NF ISO 15214 (1998) Microbiologie des aliments—Méthode horizontale pour le dénombrement des bactéries lactiques mésophiles—Technique par comptage des colonies à 30 degrés Celsius, 13.
[17]
Lango-Yaya, E. and LeBon Bondom, R. (2020) Contribution à l’étude des caractéristiques physico-chimiques et microbiologiques des boissons traditionnelles faites à base de sorgho: Cas de Bili-Bili et de son dérivé le Dora-Bonga. Afrique SCIENCE, 17, 35-46.
[18]
NF V 08-050 (2010) Microbiologie des alimentsDénombrement des coliformes présumés par comptage des colonies obtenues à 30°C, 12.
[19]
NF ISO 16649-2 (2001) Méthode horizontale pour le dénombrement des Escherichia coli bêta-glucuronidase positive-Partie 2: Technique de comptage des colonies à 44°C au moyen de 5-bromo-4-chloro3-indolyl bêta-D-glucuronate, 11.
[20]
Norme AFNOR NFT 90-401 (1996) Essais des eaux dénombrement des microorganismes reviviables à 37°C méthode par incorporation en gélose.
[21]
Norme AFNOR NFT 90-402 (1996) Essais des eaux dénombrement des microorganismes reviviables à 22°C méthode par incorporation en gélose.
[22]
Règlement (CE) n°2073/2005 de la Commission du 15 novembre 2005 concernant les critères microbiologiques applicables aux denrées alimentaires, 36.
[23]
Officiel République Algérienne démocratique et populaire (2013) Procédures; Méthodes officielles d’analyses physico-chimiques et microbiologiques. 219-246.
[24]
Norme AFNOR NFT 21567 méthode horizontale pour la recherche d’espèces de Shigella.
[25]
Maoura, N. And Mbaiguinam, M. (2006) Suivi technique, analytique et microbiologique de la «bili bili», bière traditionnelle tchadienne. Afrique SCIENCE, 2, 69-82
[26]
Aka, S., Djeni, N.T., Nguessan, K.F. and Yao, K.C. (2003) Variabilité des proprietes physicochimiques et denombrement de la flore Tchakapalo, bière traditionnelle de sorgho en Cote d’Ivoire. Afrique Science: Revue Internationale des Sciences et Technologie, 4, 274-286.
[27]
Raveloson, I. (2004) 74 Etude de la qualité microbiologique du “Koba ravina” vendu sur la voie publique à Antananarivo. Master’s Thesis, Université d’Antananarivo.
[28]
Razafy A.G.M. (1987) Essai d’étude de l’impact de la vente ambulante d’aliments dans la ville d’Antananarivo. Master’s Thesis, Université d’Antananarivo.
[29]
Adjou, E.S., Yehouenou, B., Sossou, C.M., Soumanou, M.M. and de Souza, C.A. (2012) Occurrence of Mycotoxins and Associated Mycoflora in Peanut Cake Product (Kulikuli) Marketed in Benin. African Journal of Biotechnology, 11, 14354-14360. https://doi.org/10.5897/ajb12.324
[30]
Ezekiel, C.N., Sulyok, M., Warth, B., Odebode, A.C. and Krska, R. (2012) Natural Occurrence of Mycotoxins in Peanut Cake from Nigeria. Journal Food Control, 27, 338-342. https://doi.org/10.1016/j.foodcont.2012.04.010
