Prevalence, Antimicrobial Susceptibility Pattern, and Associated Health Risks of Foodborne Pathogens in Street Foods Sold in Elementary Schools, Yaounde, Cameroon
Objectives: Street foods are an affordable source of nutrition in Cameroon; however, their association with microbial pathogens poses significant risk to public health. Methods: We investigated 160 street food samples sold within elementary schools for the presence of foodborne pathogens and also the antimicrobial resistance of cultured-confirmed bacteria isolates using the agar disk diffusion method. Results: The total viable count of seven (bread, spaghetti, fish, peanuts, eggs, beef, pepper) of the eight street foods examined (except beans sauce) were >105 cfu/g and indicated unsatisfactory levels for human consumption. In addition, 16 bacteria pathogens with at least one in each street food were identified: E. coli was the most prevalent 47.50% (76/160), followed by Staphylococcus aureus 39.37% (63/160) in foods such as beef 80% (16/20) and bread 85% (17/20). Salmonella spp. 32.50% (52/160), Campylobacter spp. 14.37% (23/160) and Shigella spp. 23.75% (38/160) were largely isolated in eggs 60% (12/20), 35% (7/10), and pepper 40% (8/20) sauces, respectively. Furthermore, except E. coli, all bacteria isolates were highly susceptible to ciprofloxacin. Yersinia enterocolitica was the most susceptible to ciprofloxacin 90.90% (10/11), tetracycline 81.81% (9/11), gentamicin 81.81% (5/11), and chloramphenicol 90.09% (10/11). Staphylococcus aureus, Campylobacter spp., and E. coli were highly resistant to chloramphenicol 90.47% (57/63), tetracycline 82.60% (19/23), gentamicin 81.57% (62/76), and vancomycin 64.47% (49/76), respectively. Conclusion: The findings strongly indicated that preventable foodborne diseases of resistance to a wide range of antibiotics could be occurring among food handlers and young children in street food vending settings. In particular, the presence of coliforms in all street food samples indicated poor hygiene and sanitary practices in street food activities. It thus recommends effective implementation strategies to improve food safety and hygiene practices in street food vending within elementary schools in Cameroon.
References
[1]
Pires, S.M., Desta, B.N., Mughini-Gras, L., Mmbaga, B.T., Fayemi, O.E., Salvador, E.M., et al. (2021) Burden of Foodborne Diseases: Think Global, Act Local. Current Opinion in Food Science, 39, 152-159. https://doi.org/10.1016/j.cofs.2021.01.006
[2]
Kirk, M.D., Angulo, F.J., Havelaar, A.H. and Black, R.E. (2016) Diarrhoeal Disease in Children Due to Contaminated Food. Bulletin of the World Health Organization, 95, 233-234. https://doi.org/10.2471/blt.16.173229
[3]
WHO (2024) Diarrhoeal Disease. https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease
[4]
Troeger, C.E., Khalil, I.A., Blacker, B.F., Biehl, M.H., Albertson, S.B., et al. (2017) Quantifying Risks and Interventions That Have Affected the Burden of Diarrhoea among Children Younger than 5 Years: An Analysis of the Global Burden of Disease Study. The Lancet Infectious Diseases, 20, 37-59.
[5]
Havelaar, A.H., Kirk, M.D., Torgerson, P.R., Gibb, H.J., Hald, T., Lake, R.J., et al. (2015) World Health Organization Global Estimates and Regional Comparisons of the Burden of Foodborne Disease in 2010. PLOS Medicine, 12, e1001923. https://doi.org/10.1371/journal.pmed.1001923
[6]
Machava, N.E., Mulaudzi, F.M. and Salvador, E.M. (2022) Household Factors of Foodborne Diarrhea in Children under Five in Two Districts of Maputo, Mozambique. International Journal of Environmental Research and Public Health, 19, Article 15600. https://doi.org/10.3390/ijerph192315600
[7]
Edima, H., Nnam, R., Enama, T., Biloa, D. and Ndjouenkeu, R. (2014) Street Food Sector in the Metropolitan Areas of a Cameroonian City, Yaounde. International Journal of Current Microbiology and Applied Sciences, 3, 740-751.
[8]
WHO (2022) Food Safety Is Everyone’s Business in Street Food Vending. https://www.who.int/publications/i/item/WHO-HEP-NFS-AFS-2022.4
[9]
Kok, R. (2014) Street Food Vending and Hygiene Practices and Implications for Consumers. Journal of Economics and Behavioral Studies, 6, 188-193. https://doi.org/10.22610/jebs.v6i3.482
[10]
Rakha, A., Fatima, M., Bano, Y., Khan, M.A., Chaudhary, N. and Aadil, R.M. (2022) Safety and Quality Perspective of Street Vended Foods in Developing Countries. Food Control, 138, Article ID: 109001. https://doi.org/10.1016/j.foodcont.2022.109001
[11]
Yongsi, H.B.N. (2018) Eating to Live or Eating to Damage One’s Health: Microbiological Risks Associated with Street-Vended Foods in a Subtropical Urban Setting (Yaoundé-Cameroon). Nutrition & Food Science International Journal, 6, 1-13. https://doi.org/10.19080/nfsij.2018.06.555695
Mengistu, D.A. and Tolera, S.T. (2020) Prevalence of Microorganisms of Public Health Significance in Ready-To-Eat Foods Sold in Developing Countries: Systematic Review and Meta-Analysis. International Journal of Food Science, 2020, Article ID: 8867250. https://doi.org/10.1155/2020/8867250
[14]
Kharel, N., Palni, U. and Tamang, J.P. (2016) Microbiological Assessment of Ethnic Street Foods of the Himalayas. Journal of Ethnic Foods, 3, 235-241. https://doi.org/10.1016/j.jef.2016.01.001
[15]
Lubis, N.D.A., Amelia, S., Arrasyid, N.K. and Rozi, M.F. (2019) Modelling of Risk Factors Associated with Foodborne Disease among School-Aged Children in Medan, Indonesia. Open Access Macedonian Journal of Medical Sciences, 7, 3302-3306. https://doi.org/10.3889/oamjms.2019.721
[16]
Djoulde, R., Bayoï, J. and Daoudou, B. (2015) Microbiological Quality and Safety of Street Meat-Food Sold in Soudano Sahelian Zone of Cameroon. International Journal of Current Microbiology and Applied Sciences (IJCMAS), 4, 441-450.
[17]
Laure Wounang Ngueugang, A., Tientche, B., Asaah, S., Lucien Fouamno Kamga, H. and Bsadjo Tchamba, G. (2021) Microbiological Assessment of Ready-to-Eat Food Sold in Urban Primary Schools, Douala, Littoral Region, Cameroon. International Journal of Food Science and Biotechnology, 6, 36-44. https://doi.org/10.11648/j.ijfsb.20210602.13
[18]
Nicholas, T., Emlah, N., Nyasa, R., Meriki, H., Mbino, T. and Kamdep, Y. (2020) Microbial Analysis and Factors Associated with Contamination of Ready-To-Eat Chili Pepper Sauce in Buea Municipality, Cameroon. African Journal of Food Science, 14, 366-372.
[19]
Tchigui Manga Maffouo, S., Tene Mouafo, H., Mouokeu, R.S., Manet, L., Kamgain Tchuenchieu, A., Noutsa Simo, B., et al. (2021) Evaluation of Sanitary Risks Associated with the Consumption of Street Food in the City of Yaoundé (Cameroon): Case of Braised Fish from Mvog-Ada, Ngoa Ekélé, Simbock, Ahala and Olézoa. Heliyon, 7, e07780. https://doi.org/10.1016/j.heliyon.2021.e07780
[20]
Wouafo, M., Pouillot, R., Kwetche, P.F., Tejiokem, M., Kamgno, J. and Fonkoua, M. (2006) An Acute Foodborne Outbreak Due to Plesiomonasshigelloides in Yaounde, Cameroon. Foodborne Pathogens and Disease, 3, 209-211. https://doi.org/10.1089/fpd.2006.3.209
[21]
WHO (2021) Estimating the Burden of Foodborne Diseases: A Practical Handbook for Countries: A Guide for Planning, Implementingand Reporting Country-Level Burden of Foodborne Disease.
[22]
WHO (2016) Estimating the Burden of Foodborne Diseases. https://www.who.int/activities/estimating-the-burden-of-foodborne-diseases
[23]
Tanyi, M.E. (2016) Pedagogic Barriers in Cameroon Inclusive Classrooms: The Impact of Curriculum, Teachers’ Attitudes and Classroom Infrastructures. Journal of Education and Practice, 7, 210-221.
[24]
Ministry of Basic Education Statistical Yearbook 2021/2022. https://www.scribd.com/document/673080537/MINEDUB-Statistical-Yearbook-2020-2021
[25]
Yongsi, H.B.N. (2008) Pathogenic Microorganisms Associated with Childhood Diarrhea in Low-And-Middle Income Countries: Case Study of Yaoundé—Cameroon. International Journal of Environmental Research and Public Health, 5, 213-229. https://doi.org/10.3390/ijerph5040213
[26]
CLSI (2020) Performance Standards for Antimicrobial Susceptibility Testing. 30th Edition, Clinical and Laboratory Standard Institute.
[27]
Microbiological Quality Guide for Ready-To-Eat Foods: A Guide to Interpreting Micro-Biological Results.
[28]
Zokou, R., Mouafo, H.T., Klang, J.M., Simo, N.B., Mouokeu, R.S. and Womeni, H.M. (2022) Microbiological Quality of Egusi Pudding, a Traditional Cake of Cucurbitaceae Sold in the City of Yaoundé, Cameroon. Journal of Food Quality, 2022, Article ID: 4236921. https://doi.org/10.1155/2022/4236921
[29]
Compaore, M.K.A., Kpoda, S.D., Bazie, R.B.S., Ouedraogo, M., Valian, M., Gampene, M., et al. (2022) Microbiological Quality Assessment of Five Common Foods Sold at Different Points of Sale in Burkina-Faso. PLOS ONE, 17, e0258435. https://doi.org/10.1371/journal.pone.0258435
[30]
Malm, K., Nyarko, K., Yawson, A., Gogo, B., Lawson, A. and Afari, E. (2015) Foodborne Illness among School Children in Ga East, Accra. Ghana Medical Journal, 49, 72-76. https://doi.org/10.4314/gmj.v49i2.2
[31]
Tanyitiku, M.N., Nicholas, G., Petcheu, I.C.N., Sullivan, J.J. and On, S.L.W. (2022) Public Health Risk of Foodborne Pathogens in Edible African Land Snails, Cameroon. Emerging Infectious Diseases, 28, 1715-1717. https://doi.org/10.3201/eid2808.220722
[32]
Tanyitiku, M.N., Teh, E.A., Laison, R.M. and Petcheu, I.C.N. (2023) Foodborne Pathogens in Leafy Vegetables Grown and Consumed Locally in Yaounde, Cameroon: A Public Health Concern. Journal of Food Research, 12, 11-22. https://doi.org/10.5539/jfr.v12n2p11
[33]
UKHSA (2024) Guidelines for Assessing the Microbiological Safety of Ready-To-Eat Foods. Health Protection Agency. https://assets.publishing.service.gov.uk/media/5a7efde0e5274a2e8ab497a4/Guidelines_for_assessing_the_microbiological_safety_of_ready-to-eat_foods_on_the_market.pdf
[34]
Tschäpe, H., Prager, R., Streckel, W., Fruth, A., Tietze, E. and Böhme, G. (1995) Verotoxinogenic Citrobacter Freundii associated with Severe Gastroenteritis and Cases of Haemolytic Uraemic Syndrome in a Nursery School: Green Butter as the Infection Source. Epidemiology and Infection, 114, 441-450. https://doi.org/10.1017/s0950268800052158
[35]
Sivalingam, K.M., Sama, D.S. and Vaithilingam, S. (2024) Prevalence and Antibiotic Susceptibility Pattern of Foodborne Bacterial Pathogens Isolated from Spoiled Vegetables at Wolaita Sodo Town, South Ethiopia. Journal of Food Quality, 2024, Article ID: 8823056. https://doi.org/10.1155/jfq/8823056
[36]
Kidsley, A.K., Abraham, S., Bell, J.M., O'Dea, M., Laird, T.J., Jordan, D., et al. (2018) Antimicrobial Susceptibility of Escherichia Coli and Salmonella Spp. Isolates from Healthy Pigs in Australia: Results of a Pilot National Survey. Frontiers in Microbiology, 9, Article 1207. https://doi.org/10.3389/fmicb.2018.01207
[37]
GRAM (2019) The Burden of Antimicrobial Resistance (AMR) in Cameroon. Institute for Health Metrics and Evaluation. https://www.healthdata.org/sites/default/files/2023-09/Cameroon.pdf
[38]
Ngogang, M.P., Nkoth, A.f., Ngaleu, W., Mfouapon, H., Ekoume, P., Nibeye, Y., et al. (2024) Antimicrobial Susceptibility Testing Data Analysis over 3 Years at the Yaoundé General Hospital, Cameroon. JAC-Antimicrobial Resistance, 6, dlae043. https://doi.org/10.1093/jacamr/dlae043
[39]
Mancuso, G., Midiri, A., Gerace, E. and Biondo, C. (2021) Bacterial Antibiotic Resistance: The Most Critical Pathogens. Pathogens, 10, Article 1310. https://doi.org/10.3390/pathogens10101310
[40]
Scallan, E., Mahon, B.E., Hoekstra, R.M. and Griffin, P.M. (2013) Estimates of Illnesses, Hospitalizations and Deaths Caused by Major Bacterial Enteric Pathogens in Young Children in the United States. Pediatric Infectious Disease Journal, 32, 217-221. https://doi.org/10.1097/inf.0b013e31827ca763
[41]
Tack, D.M., Marder, E.P., Griffin, P.M., Cieslak, P.R., Dunn, J., Hurd, S., et al. (2019) Preliminary Incidence and Trends of Infections with Pathogens Transmitted Commonly through Food—Foodborne Diseases Active Surveillance Network, 10 U.S. Sites, 2015-2018. MMWR. Morbidity and Mortality Weekly Report, 68, 369-373. https://doi.org/10.15585/mmwr.mm6816a2
[42]
(2024) Young Children and Foodborne Illness. A Fact Sheet from the Pew Charitable Trusts, American Academy of Pediatrics, and the Center for Foodborne Illness. https://www.pewtrusts.org/foodsafety
[43]
Vo, T.H., Le, N.H., Cao, T.T.D., Nuorti, J.P. and Minh, N.N.T. (2014) An Outbreak of Food-Borne Salmonellosis Linked to a Bread Takeaway Shop in Ben Tre City, Vietnam. International Journal of Infectious Diseases, 26, 128-131. https://doi.org/10.1016/j.ijid.2014.05.023
[44]
Kimura, A.C., Palumbo, M.S., Meyers, H., Abbott, S., Rodriguez, R. and Werner, S.B. (2005) A Multi-State Outbreak of salmonella Serotype Thompson Infection from Commercially Distributed Bread Contaminated by an Ill Food Handler. Epidemiology and Infection, 133, 823-828. https://doi.org/10.1017/s0950268805004127
[45]
Lathrop, A.A., Taylor, T. and Schnepf, J. (2014) Survival of Salmonella during Baking of Peanut Butter Cookies. Journal of Food Protection, 77, 635-639. https://doi.org/10.4315/0362-028x.jfp-13-408
