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基于双样本孟德尔随机化探究食源性致病菌与胃肠道癌症转移的相关性
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Abstract:
研究利用孟德尔随机化法(Mendelian randomization, MR)分析了几种食源性病原体与白细胞分化抗原44 (Cluster of differentiation 44, CD44)之间的关联，CD44抗原是胃肠道癌症发展和转移的标志物。该研究从综合流行病学数据库(IEU)、芬兰基因数据库(FinnGen)和英国生物数据库(UK Biobank)获得了全基因组关联研究(Genome-wide association studies, GWAS)数据，并主要使用反方差加权(Inverse-variance weighting, IVW)等统计方法分析了相关性。同时还进行了敏感性分析，以提高结果的可靠性。研究结果表明，幽门螺旋杆菌与CD44抗原呈显著正相关，而小肠结肠耶尔森氏菌与CD44抗原呈显著性负相关，因此研究判断幽门螺旋杆菌会促进癌症的发生和转移，而小肠结肠耶尔森氏菌可抑制癌细胞的发展和转移。然而，其余细菌种类没有发现明显的相关性。部分食源性致病菌可能会促进胃肠道癌症的转移，如幽门螺旋杆菌。但还有些可能抑制了癌细胞的转移，表明背后可能有更复杂的生理机制。
This study used Mendelian randomization (MR) to analyze the association between several foodborne pathogens and the Cluster of Differentiation 44 (CD44) antigen, a marker of gastrointestinal cancer development and metastasis. The study obtained genome-wide association studies (GWAS) data from Integrative Epidemiology Unit (IEU), FinnGen and UK Biobank and analyzed the correlation mainly using statistical methods such as inverse-variance weighted (IVW). Sensitivity analyses were also carried out to improve the reliability of the results. The results of the study showed that Helicobacter pylori showed a significant positive correlation with CD44 antigen; Yersinia enterocolitica showed a significant negative correlation with CD44 antigen; therefore, we judged that Helicobacter pylori promotes cancer development and metastasis, and Yersinia enterocolitica inhibits cancer cell development and metastasis. However, no significant correlation was found for the remaining bacterial species. Some foodborne pathogens, such as Helicobacter pylori, can promote the metastasis of gastrointestinal cancers. However, others can inhibit the metastasis of cancer cells, suggesting that there may be a more complex physiological mechanism involved.
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