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日本海神蛤壳修复过程中外套膜组织的转录组特征解析
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Abstract:
为探究日本海神蛤壳体破损后外套膜修复组织与正常外套膜组织的差异，本研究利用转录组测序技术(RNA-Seq)及生物信息学分析方法，比较分析外套膜修复组织(A4Y1)、外套膜外部组织(A4Wa1)和外套膜内部组织(A4Wb1)转录组。结果显示，在三种外套膜组织中共筛选出1864个差异表达基因(DEGs) (筛选条件：p.adj < 0.05且|log2FoldChange| ≥ 1)。其中，A4Wa1与A4Y1中上调表达基因807个，下调表达基因598个；A4Wb1与A4Y1中上调表达基因678个，下调表达基因563；A4Wa1与A4Wb1中上调表达基因44，下调表达基因142。GO和KEGG富集分析显示，这些差异基因显著富集于生物矿化和免疫相关的通路中。且相关基因在修复组织中表达水平较高。这些结果显示，三种外套膜组织在生物矿化和免疫功能方面存在显著差异，这可能与日本海神蛤壳体修复机制密切相关。本研究为深入探讨日本海神蛤外套膜功能提供了数据支持，同时为其他软体生物外套膜的研究提供了参考。
To investigate the differences between mantle repair tissue and normal mantle tissue of Panopea japonica following shell damage, this study employed RNA-Seq and bioinformatics analysis to compare the transcriptomes of mantle repair tissue (A4Y1), external mantle tissue (A4Wa1), and internal mantle tissue (A4Wb1). A total of 1864 differentially expressed genes (DEGs) were identified across the three types of mantle tissues (selection criteria: p.adj < 0.05 and |log2FoldChange| ≥ 1). Among these, 807 upregulated and 598 downregulated genes were found in A4Wa1 vs. A4Y1, 678 upregulated and 563 downregulated genes in A4Wb1 vs. A4Y1, and 44 upregulated and 142 downregulated genes in A4Wa1 vs. A4Wb1. GO and KEGG enrichment analyses revealed significant enrichment of these DEGs in pathways associated with biomineralization and immunity, with higher expression levels in repair tissues. These findings indicate that the three mantle tissue types exhibit significant differences in biomineralization and immune functions, which are likely linked to the shell repair mechanisms of P. japonica. This study provides data support for further exploration of mantle functions in P. japonica and serves as a reference for research on mantles in other mollusks.
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