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连梅汤对LPS诱导的脓毒症小鼠的保护作用研究
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Abstract:
目的：由连梅汤(LMD)对脂多糖(LPS)诱导的脓毒症小鼠回肠屏障及肝肺损伤的保护作用的研究。方法：采用随机方式，将C57/6J雄性小鼠分为对照组(CON组)、LPS模型组(LPS组)和连梅汤治疗组(LMD组)，每组各6只。适应性喂养7天后，分别经口给予生理盐水与治疗剂量的连梅汤(LMD) 21天。第22天，PBS注入对照组腹腔，另两组LPS注入5 mg/kg腹腔，建立脓毒症模型小鼠。腹腔注射24小时后进行回肠和肝、肺组织的收集。用HE染色组织病理鉴定；通过RT-qPCR检测回肠屏障因子水平(ZO-1, Occludin)和肝肺组织炎性因子(IL-1α, IL-8, TNF-α)。结果LPS组与CON相比，体重下降明显(n = 6; P < 0.0001)，小鼠临床评分大幅提高(n = 6; P < 0.0001)。与LPS组相比，LMD可显著提高回肠屏障因子ZO-1、Occludin的表达(n = 6; P < 0.0001)，下调肝、肺组织的促炎因子(IL-1β、IL-8、TNF-α)水平(n = 6; P < 0.05)，减轻肝、肺组织的病理损伤。结论：连梅汤能有效改善LPS诱导的脓毒症小鼠回肠屏障损伤和肝、肺损伤及炎症失衡。
Objective: The protective effect of Lianmei Decoction on the intestine was studied to the intestinal barrier, liver, and lung damage caused by lipopolysaccharide (LPS) in septic mice. Methods: C57/6J male mice were randomly assigned to the control group (CON group), LPS model group (LPS group), and Lianmei Decoction treatment group (LMD group), with 6 mice in each group. After 7 days of adaptive feeding, normal saline and a therapeutic dose of LMD were given orally for 21 days. In the 22 days of the study, PBS was injected intraperitoneally into the CON group, and 5 mg/kg LPS was administered intraperitoneally to the remaining two groups to create a sepsis mouse model. Ileum, liver, and lung tissues were gathered 24 hours after intraperitoneal injection. Histopathological examination was done using HE staining; the amounts of ileal barrier factors (ZO-1, Occludin) and inflammatory factors (IL-1β, IL-8, TNF-α) in liver and lung tissues were detected by RT-qPCR. Results: Compared with the CON group, the body weight of the LPS group decreased dramatically (n = 6; P < 0.0001), and the clinical score of mice was considerably raised (n = 6; P < 0.0001). Compared with the LPS group, LMD significantly increased the expression of ileal intestinal barrier factors ZO-1 and Occludin (n = 6; P < 0.0001), down-regulated the levels of pro-inflammatory factors (IL-1β, IL-8, TNF-a) in liver and lung tissues (n = 6; P < 0.05), reduce the pathological damage of liver and pulmonary tissue. Conclusion: Lianmei Decoction can effectively improve ileal barrier damage, liver and lung injury, and inflammatory imbalance in LPS-induced sepsis mice.
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