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口服双歧杆菌四联活菌片对机械通气患者血清非靶向代谢组学的影响
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Abstract:
目的：分析口服双歧杆菌四联活菌片对临床机械通气患者血清非靶向代谢组学和短链脂肪酸生成的影响，探讨双歧杆菌四联活菌片增强患者黏膜免疫力机制。方法：10例重症患者在给予常规治疗的同时口服双歧杆菌四联活菌片。治疗14天后收集血清用于降钙素原(PCT)、IL-6、IL-17、IgA水平检测，粪便用于3种主要短链脂肪酸(乙酸、丙酸和丁酸)浓度检测，采用超高效液相色谱–四极杆–飞行时间质谱(UPLC-Q-TOF/MS)技术分析患者血清非靶向代谢组学变化。结果：益生菌疗法处理后患者血清中PCT和IL-17水平较处理前显著降低，IgA浓度明显升高(P < 0.05)，但粪便中3种短链脂肪酸浓度变化不显著(P > 0.05)。益生菌疗法可以显著影响患者亚油酸代谢、甘油磷脂代谢和色氨酸代谢。结论：口服双歧杆菌四联活菌片可以明显抑制患者体内炎症反应并增加IgA表达量，并且可以显著影响血清非靶向代谢组学。益生菌疗法可能通过亚油酸代谢、甘油磷脂代谢和色氨酸代谢途径抑制炎症并增强黏膜免疫力。
Objective: To analyze the effects of oral quadruple live bifidobacterium tablets on the serum non-targeted metabolomics and short-chain fatty acids production in clinical mechanically ventilated patients, and explore the mechanism by which probiotics enhance mucosal immune function in patients. Methods: Ten critically ill patients were given oral quadruple live bifidobacterium tablets while receiving routine treatment. After 14 days of treatment, serum was collected for measurement of procalcitonin (PCT), IL-6, IL-17, and IgA levels, and feces were collected for measurement of concentrations of three main short-chain fatty acids (acetic acid, propionic acid, and butyric acid). Ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) technology was used to analyze changes in the serum non-targeted metabolomics of the patients. Results: After probiotic therapy, the levels of PCT and IL-17 in the serum of patients were significantly reduced compared to before treatment, while IgA concentration significantly increased (P < 0.05), but the concentrations of the three short-chain fatty acids in the feces did not change significantly (P > 0.05). Probiotic therapy significantly affected the metabolism of arachidonic acid, glycerophospholipids, and tryptophan in patients. Conclusion: oral quadruple live bifidobacterium tablets can significantly inhibit inflammation in the body and increase IgA expression levels in patients, and can significantly affect serum non-targeted metabolomics. Probiotic therapy may inhibit inflammation and enhance mucosal immunity through the pathways of arachidonic acid metabolism, glycerophospholipid metabolism, and tryptophan metabolism.
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