As a well-recognized environmental contaminant, lead (Pb) represents a profound threat to human health, with the nervous system being especially vulnerable. Pb exerts its toxic effects through a range of specific mechanisms, such as inducing apoptosis, promoting oxidative stress, disrupting ion channel function, impairing neurotransmitter systems, and causing microglial cell damage within neurons. Previous studies have demonstrated that Walnut Peel Extracts possess substantial antioxidant properties. However, varying extraction methods and solvents yield different bioactive compounds. In our study, we employed varying concentrations of Walnut Peel Water-Soluble Extracts (WPWE) to counteract Pb toxicity. Specifically, we utilized the MTT assay, Hoechst staining, and fluorescence probes to assess cell viability, apoptosis rates, and ROS levels. The results indicate that WPWE can effectively mitigate the toxic effects of Pb on PC-12 cells.
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