|
|
不同黑种草子挥发油中百里醌的含量
|
Abstract:
目的：测定不同产地黑种草子挥发油中百里醌的含量。方法：将5种不同产地的黑种草子用两种不同化学提取方法提取挥发油，建立定量核磁共振波谱法测定挥发油中的百里醌。使用500 MHZ Bruker Avance III (TMS, δ = 0 ppm)核磁共振谱仪，以氖代氯仿为溶剂，在测定温度25℃、延迟时间2 s和扫描次数64的条件下扫描，通过化学移位及峰面积归一化法测定各黑种草子挥发油中百里醌相对百分比含量。结果：以化学位移在δ = 6.5 ppm与δ = 6.6 ppm处的百里醌的氢质子峰作为定量峰，其峰面积比与其质量比的线性回归方程为Y = 0.493x ？ 0.0005，相关系数为0.999。5种不同产地黑种草子挥发油中百里醌在方法1中测得USA1 (0.065%)、USA2 (0.093%)、USA3 (0.043%)、Tibe (0.043%)、Guangxi (0.062%)。在方法2中测得USA1 (0.263%)、USA2 (0.153%)、USA3 (0.037%)、Tibet (0.200%)、Guangxi (0.224%)。结论：分析结果表明，因产地及提取方法差异，黑种草子挥发油中百里醌含量测定是有差异的。文章通过测定不同产地黑种草子挥发油中的百里醌含量，为进一步研究开发黑种草子的药用价值奠定基础。
Objective: To determine the content of quinone in volatile oil from black seed (Nigella) of different habitats. Methods: The essential oils were extracted from the seeds of black grass from five different habitats by two different chemical extraction methods, and the thymoquinone in the essential oils was determined by quantitative nuclear magnetic resonance spectroscopy. A 500 MHz Bruker Avance III (TMS, δ = 0 ppm) nuclear magnetic resonance spectrometer was used, with deuterochloroform as the solvent, at a measurement temperature of 25？C, a delay time of 2 seconds, and 64 scans, to determine the relative percentage content of thymoquinone in the volatile oils of black grass seeds by chemical shift and peak area normalization. Results: Using the hydrogen proton peak of thymoquinone with a chemical shift at δ = 6.5 ppm and δ = 6.6 ppm as the quantitative peak, the peak area ratio of thymoquinone and its mass ratio were linear regression equation Y = 0.493x ？ 0.0005, the correlation coefficient was 0.999, and thymoquinone in volatile oils of black grass from five different habitats was determined as USA1 (0.065%), USA2 (0.093%), USA3 (0.043%), Tibet (0.043%), Guangxi (0.062%) in method 1. In method 2, USA1 (0.263%), USA2 (0.153%), USA3 (0.037%), Tibet (0.200%), and Guangxi (0.224%) were measured. Conclusion: The results showed that there were differences in the determination of thymoquinone content in the essential oil of Herba Nigella due to the differences in origin and extraction methods. In this paper, the content of thymoquinone in the essential oil of black seed grass from different habitats was determined to lay a foundation for further research and development of the medicinal value of black seed grass.
| [1] | Xin, X.L., Xue, H.Q., Aisa, H.A., et al. (2008) Research Development of the Plants of Nigella Gerus. Lishizhen Medicine and Materia Medica Research, 19, 1514-1517. |
| [2] | 内蒙古自治区卫生厅. 内蒙古蒙药材标准[M]. 赤峰: 内蒙古科学技术出版社, 1987: 94-95. |
| [3] | 布和巴特尔. 传统蒙药与方剂[M]. 赤峰: 内蒙古科学技术出版社, 2013: 65. |
| [4] | Mohamed, A., Afridi, D.M., Garani, O., et al. (2005) Thymoquinone Inhibits the Activation of NF-κB in the Brain and Spinal Cord of Experimental Autoimmune Encephalomyelitis. Biomedical Sciences Instrumentation, 41, 388-393. |
| [5] | Woo, C.C., Kumar, A.P., Sethi, G. and Tan, K.H.B. (2012) Thymoquinone: Potential Cure for Inflammatory Disorders and Cancer. Biochemical Pharmacology, 83, 443-451. https://doi.org/10.1016/j.bcp.2011.09.029 |
| [6] | Sakamoto, J., Matsui, T. and Kodera, Y. (2009) Paclitaxel Chemotherapy for the Treatment of Gastric Cancer. Gastric Cancer, 12, 69-78. https://doi.org/10.1007/s10120-009-0505-z |
| [7] | Sharma, R., Gupta, P.K., Mazumder, A., Dubey, D.K., Ganesan, K. and Vijayaraghavan, R. (2009) A Quantitative NMR Protocol for the Simultaneous Analysis of Atropine and Obidoxime in Parenteral Injection Devices. Journal of Pharmaceutical and Biomedical Analysis, 49, 1092-1096. https://doi.org/10.1016/j.jpba.2009.01.035 |
| [8] | 高照明, 张玉冰, 于永良. 核磁共振内标法定量分析肝素钠中多硫酸软骨素[J]. 分析化学, 2011, 39(4): 601-602. |
| [9] | 郭强胜, 石高旗, 宋巍, 许旭. 马来酸氯苯那敏片剂的核磁共振定量测定方法研究[J]. 上海应用技术学院学报(自然科学版), 2011, 11(2): 123-128. |
| [10] | 郭强胜, 石高旗, 宋巍, 等. NMR定量测定片剂中的对乙酰氨基酚[J]. 分析测试学报, 2012, 31(1): 117-120. |
| [11] | 张才煜, 张娜, 何兰. 核磁共振内标法测定波生坦的含量[J]. 药学学报, 2014, 49(2): 249-251. |
| [12] | 高翔, 陈东军, 马艳春, 等. 消癌平注射液中牛奶菜醇的NMR定量分析[J]. 波谱学杂志, 2012, 29(3): 410-418. |
| [13] | 易进海, 刘云华, 陈燕, 等. 核磁共振波谱法测定藁本内酯对照品的含量[J]. 药物分析杂志, 2010, 30(4): 680-682. |
| [14] | 杨扬, 潘勤, 丁黎, 吴晓磊. QNMR法测定川芎药材中的有效成分[J]. 华西药学杂志, 2013, 28(2): 192-194. |
| [15] | Fan, G., Zhang, M.Y., Zhou, X.D., Lai, X.R., Yue, Q.H., Tang, C., et al. (2012) Quality Evaluation and Species Differentiation of Rhizoma Coptidis by Using Proton Nuclear Magnetic Resonance Spectroscopy. Analytica Chimica Acta, 747, 76-83. https://doi.org/10.1016/j.aca.2012.08.038 |
| [16] | Staneva, J., Denkova, P., Todorova, M. and Evstatieva, L. (2011) Quantitative Analysis of Sesquiterpene Lactones in Extract of Arnica Montana L. by 1H NMR Spectroscopy. Journal of Pharmaceutical and Biomedical Analysis, 54, 94-99. https://doi.org/10.1016/j.jpba.2010.08.018 |
| [17] | Chauthe, S.K., Sharma, R.J., Aqil, F., Gupta, R.C. and Singh, I.P. (2012) Quantitative NMR: An Applicable Method for Quantitative Analysis of Medicinal Plant Extracts and Herbal Products. Phytochemical Analysis, 23, 689-696. https://doi.org/10.1002/pca.2375 |
| [18] | Salem, A.A., Abdou, I.M. and Saleh, H.A. (2012) Application of Quantitative Nuclear Magnetic Resonance Spectroscopy for the Determination of Amantadine and Acyclovir in Plasma and Pharmaceutical Samples. Journal of AOAC International, 95, 1644-1651. https://doi.org/10.5740/jaoacint.11-165 |
| [19] | 杨亮, 茹阁英, 唐惠儒, 刘朝阳. 核磁共振电信号内标法在人体尿液定量分析中的应用[J]. 分析化学, 2010, 38(6): 789-794. |
| [20] | Li, J., Geng, Z.F., Liu, P. and Deng, Z.W. (2012) NMR Analysis of a Pair of Isomers. Chinese Chemical Letters, 23, 1181-1184. https://doi.org/10.1016/j.cclet.2012.07.008 |
| [21] | del Campo, G., Berregi, I., Caracena, R. and Zuriarrain, J. (2010) Quantitative Determination of Caffeine, Formic Acid, Trigonelline and 5-(Hydroxymethyl)Furfural in Soluble Coffees by 1H NMR Spectrometry. Talanta, 81, 367-371. https://doi.org/10.1016/j.talanta.2009.12.010 |
| [22] | Schievano, E., Guardini, K. and Mammi, S. (2009) Fast Determination of Histamine in Cheese by Nuclear Magnetic Resonance (NMR). Journal of Agricultural and Food Chemistry, 57, 2647-2652. https://doi.org/10.1021/jf803364k |
| [23] | 耿东升, 张淑锋, 兰建国, 等. 瘤果黑种草子挥发油的化学成分分析及百里醌的定量[J]. 中国中药杂志, 2009, 34(22): 2287-2290. |
| [24] | Zhivotovsky, B. (2006) Carcinogenesis and Apoptosis: Paradigms and Paradoxes. Carcinogenesis, 27, 1939-1945. https://doi.org/10.1093/carcin/bgl035 |
