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实验动物的常见采血方法综述
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Abstract:
采血作为动物实验和研究中不可或缺的一个环节，对最终实验数据的可靠性和准确性有着至关重要的作用。合适的采血方法，不仅可以降低由个体差异所造成的实验误差和实验动物的用量，还能够提高实验效率。本文主要回顾总结了针对不同环境下实验动物的采血量的选择和常用采血方法的介绍，注重医学伦理，推进实验动物科学化进程，提高相关实验采集数据的安全有效性和结果准确性。
Blood collection, as an indispensable part of animal experiments and research, plays a crucial role in the rationality and accuracy of the final experimental data. Finding a suitable blood collection method can reduce experimental errors caused by individual differences and the amount of experimental animals used, and also improve experimental efficiency. This treatise primarily appraised and encapsulated the determination of blood collection volumes for experimental animals across diverse environmental contexts, along with an explication of prevalent blood withdrawal methodologies. It placed particular emphasis on the principles of medical ethics, thereby enhancing the scientific rigor of experimental animal research and elevating the reliability and precision of the resultant experimental data and findings.
| [1] | 赵巧辉, 刘孟洲. 实验动物采血方法[J]. 上海畜牧兽医通讯, 2006(5): 4-6. |
| [2] | 杨健莉, 刘佳, 郑志红. 常用实验大小鼠采血方法及其对实验动物福利的影响[J]. 中国比较医学杂志, 2019, 29(1): 90-94. |
| [3] | Francisco, C.C., Howarth, G.S. and Whittaker, A.L. (2015) Effects on Animal Wellbeing and Sample Quality of 2 Techniques for Collecting Blood from the Facial Vein of Mice. American Association for Laboratory Animal Science, 54, 76-80. |
| [4] | van Herck, H., Baumans, V., Brandt, C.J.W.M., Hesp, A.P.M., Sturkenboom, J.H., van Lith, H.A., et al. (1998) Orbital Sinus Blood Sampling in Rats as Performed by Different Animal Technicians: The Influence of Technique and Expertise. Laboratory Animals, 32, 377-386. https://doi.org/10.1258/002367798780599794 |
| [5] | 韩穆轩, 邢影, 王岳睿, 等. 大鼠颌下静脉丛采血技术方法及其效果比较[J]. 中国比较医学杂志, 2023, 33(4): 104-108. |
| [6] | 马征, 刘秀英, 黄凰. 两种采血方法对大鼠生化和血相指标的影响研究[J]. 湖南师范大学学报(医学版), 2012, 9(2): 44-46. |
| [7] | 黄浙学, 朱青青, 余雨滋, 等. 大小鼠多种连续微量采血法的比较[J]. 江西师范大学学报(自然科学版), 2022, 46(2): 164-170. |
| [8] | Watanabe, A., Watari, R., Ogawa, K., Shimizu, R., Tanaka, Y., Takai, N., et al. (2015) Using Improved Serial Blood Sampling Method of Mice to Study Pharmacokinetics and Drug-Drug Interaction. Journal of Pharmaceutical Sciences, 104, 955-961. https://doi.org/10.1002/jps.24236 |
| [9] | 杜飞飞, 王凤清, 贾伟伟, 等. 啮齿类动物插管技术用于药动学采血的研究进展[J]. 中国实验动物学报, 2020, 28(1): 129-136. |
| [10] | Yoburn, B.C., Morales, R. and Inturrisi, C.E. (1984) Chronic Vascular Catheterization in the Rat: Comparison of Three Techniques. Physiology & Behavior, 33, 89-94. https://doi.org/10.1016/0031-9384(84)90018-0 |
| [11] | Doeing, D.C., Borowicz, J.L. and Crockett, E.T. (2003) Gender Dimorphism in Differential Peripheral Blood Leukocyte Counts in Mice Using Cardiac, Tail, Foot, and Saphenous Vein Puncture Methods. BMC Clinical Pathology, 3, Article No. 3. https://doi.org/10.1186/1472-6890-3-3 |
| [12] | 刘帅, 李军延, 陈巍. 不同采血方法Wistar大鼠血常规的影响[J]. 实验动物科学, 2014, 31(2): 32-34. |
| [13] | Omorodola, I.A., Kathleen, B.W. and Jean, A.N. (2008) Evaluation of Saphenous Venipuncture and Modified Tail-Clip Blood Collection in Mice. Journal of the American Association for Laboratory Animal Science, 47, 8-15. |
