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四妙汤对RA模型大鼠关节VDR、CYP27B1、CYP24A1、Foxp3、RORγt的影响
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Abstract:
目的：探讨四妙汤改善类风湿关节炎(RA)模型大鼠的作用机制。方法：采用胶原诱导法(CIA)建立RA模型大鼠，灌胃四妙汤水煎液低、中、高剂量组(3.15 g/kg、6.3 g/kg、12.6 g/kg)，维生素D组(25 μg/kg)。给药周期为28天，记录大鼠一般情况。苏木精–伊红(HE)染色法观察踝关节组织病理改变；酶联免疫吸附剂检测法(ELISA)检测大鼠血清白细胞介素-17 (IL-17)、白细胞介素-10 (IL-10)、转化生长因子-β (TGF-β)含量；实时荧光定量PCR法(RT-PCR)检测大鼠关节滑膜组织中维生素D受体(VDR)、1-α羟化酶(CYP27B1)、24-羟化酶(CYP24A1)、叉头/翼状螺旋转录因子3 (Foxp3)、维甲酸相关孤儿核受体γt (RORγt) mRNA表达。结果：与空白组相比，模型组血清IL-17含量水平显著上升(P < 0.05)，血清IL-10、TGF-β含量水平显著下降(P < 0.05)；关节滑膜CYP24A1、RORγt mRNA表达水平显著上升(P < 0.05)，VDR、CYP27B1、Foxp3 mRNA表达水平显著下降(P < 0.05)。与模型组相比，维生素D组及四妙汤高剂量组血清IL-17水平显著下降(P < 0.05)，维生素D组及四妙汤中、高剂量组血清IL-10水平显著上升(P < 0.05)，维生素D组及四妙汤高剂量组血清TGF-β水平显著上升(P < 0.05)；维生素D组及四妙汤中、高剂量组关节滑膜VDR、CYP27B1、Foxp3 mRNA表达水平显著上升(P < 0.05)，维生素D组及四妙汤低、中、高剂量组关节滑膜CYP24A1 mRNA表达水平显著下降(P < 0.05)，维生素D组及四妙汤中、高剂量组关节滑膜RORγt mRNA表达水平显著下降(P < 0.05)。结论：四妙汤通过调节关节滑膜组织中VDR、CYP27B1、CYP24A1、Foxp3、RORγt mRNA表达情况，改善血清IL-17、IL-10、TGF-β水平，发挥其干预RA的作用。
Objective: To explore the mechanism of action of Simiao decoction in improving the rat model of RA. Methods: Rat models of rheumatoid arthritis (RA) were established by collagen-induced arthritis (CIA). The rats were then administered with low, medium and high doses of Simiao decoction (3.15 g/kg, 6.3 g/kg, 12.6 g/kg) and vitamin D (25 ng/kg) by gavage. The administration period was 28 days, and the general conditions of the rats were recorded. Observation of pathological changes in ankle joint tissues by hematoxylin-eosin (HE) staining method. The contents of interleukin-17 (IL-17), interleukin-10 (IL-10), and transforming growth factor-β (TGF-β) in the serum of rats were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of VDR, CYP27B1, CYP24A1, Foxp3, and RORγt in the synovial tissue of rat joints were detected by real-time fluorescence quantitative PCR (RT-PCR). Results: Compared with the control group, the serum IL-17 content level in the model group significantly increased (P < 0.05), while the serum IL-10 and TGF-β content levels significantly decreased (P < 0.05); the mRNA expression levels of CYP24A1 and RORγt in the synovial membrane significantly increased (P < 0.05), and the
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