Today, agricultural production is threatened by crop pathogens, including plant-parasitic nematodes. Because of their harmful effects on the environment and human health, synthetic nematicides are gradually being banned in several countries. This study evaluates the nematicidal activity of Datura metel oil. Datura metel seed oil was obtained using the Soxhlet extractor in hexane. The resulting oil was characterized by determining physicochemical parameters and molecular composition using GC-MS. The nematicidal activity of the oil was assessed by determining the number of dead nematodes. Physicochemical characterization gave an acidity index of 0.3% and a peroxide index of 10 meq.O2/Kg, while GC-MS analysis identified 30 molecules made up mainly of fatty acid esters, four of which represented over 74% of the oil’s total weight. The nematicidal activity of the oil, expressed in terms of mortality rate as a function of concentration, showed mortality rates of 58; 69 and 79% over 48 hours of incubation at concentrations of 25, 50 and 100 μg/mL respectively. The activity observed could be linked to the high presence of the four compounds most commonly identified in the oil. These results suggest that Datura metel oil could be a promising alternative to synthetic pesticides for the control of crop pests.
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