Studying the Interaction between Chromobacteriumanophelis and the Entomopathogenic Fungus Metarhiziumpingshaense in Aedesaegypti, the Dengue Vector in Burkina Faso
Insect-bacteria associations can influence vector competence in multiple ways. Chromobacteriumanophelis, a bacterium known to be pathogenic to mosquitoes, may also act as an anti-pathogen by inhibiting the development of other pathogens within mosquitoes. The mechanism behind this inhibition remains unclear, with two hypotheses: the bacterium either boosts the mosquito’s immunity or directly targets other pathogens within the mosquito. The objective of this study is to elucidate the mechanism behind this pathogen-inhibition effect in mosquitoes. Bioassays (assessing longevity, fecundity, and fertility) were conducted on mosquitoes infected with the bacterium and an entomopathogenic fungus, Metarhiziumpingshaense, whose hyphae grow on mosquitoes after killing them. The prevalence of hyphal growth was evaluated. The longevity of mosquitoes co-infected with Chromobacteriumanophelis and Metarhiziumpingshaense was significantly higher than those infected solely with the more virulent microorganism, which was Chromobacteriumanophelis. Hyphae were observed on 100% of mosquitoes infected only with the fungus, whereas mosquitoes co-infected with the bacterium exhibited a lower prevalence of fungal hyphal growth. The number of eggs laid by infected mosquitoes was approximately the same, within the typical range (50 - 150). However, the number of larvae observed from co-infected mosquitoes was significantly higher than those produced by mosquitoes exposed to the microorganism that most reduced egg hatch rates. These results align with the hypothesis that Chromobacteriumanophelis inhibits the development of other pathogens within mosquitoes by directly targeting them.
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