吴茱萸碱与吴茱萸次碱对T2DM SD大鼠肝损伤的影响
The Effect of Evodiamine and Rutaecarpine Hypoallergenic Base on Liver Injury in T2DM SD Rats

目的：本研究旨在评估吴茱萸碱(Evodiamine, EVO)与吴茱萸次碱(Rutaecarpine, RUT)对2型糖尿病(Type 2 Diabetes Mellitus, T2DM)SD大鼠肝损伤的治疗潜力。方法：通过高脂高糖饲料喂养联合腹腔注射链脲佐菌素(Streptozotocin, STZ)的方法，建立了T2DM大鼠肝损伤模型。实验选取了35只健康SPF级SD雄性大鼠，并随机分为空白组、模型组、盐酸二甲双胍组(MET)，EVO组和RUT组。给药4周后，测定了各组大鼠的FBG、血脂四项、肝糖原、肝糖原合成酶(GS)、葡萄糖-6-磷酸酶(G-6-Pase)及天冬氨酸氨基转移酶(AST)水平，并结合肝脏组织病理学分析进行了综合评估。结果：与模型组相比，EVO和RUT显著降低FBG，缓解体重减轻，改善血脂代谢，调节肝脏糖代谢(肝糖原、GS、G-6-Pase水平下降)，降低AST水平，表明具有保肝作用。肝脏组织病理学分析显示，EVO和RUT能减轻肝细胞脂肪变性和炎症浸润，增加肝糖原沉淀，有效缓解肝损伤。结论：吴茱萸碱(EVO)与吴茱萸次碱(RUT)对T2DM大鼠肝损伤具有显著治疗潜力，能降血糖、调血脂、调节肝脏糖代谢并保护肝脏组织，为2型抗糖尿病药物开发提供实验依据。
Objective: The aim of this study was to evaluate the therapeutic potential of Evodiamine (EVO) versus Rutaecarpine (RUT) on liver injury in SD rats with Type 2 Diabetes Mellitus (T2DM). Methods: A liver injury model of T2DM rats was established through a combination of feeding a high-fat, high-sugar diet and intraperitoneal injection of streptozotocin (STZ). In the experiment, 35 healthy male SPF-grade SD rats were selected and randomly divided into a blank group, a model group, a metformin hydrochloride group (MET), an EVO group, and a RUT group. After 4 weeks of drug administration, the levels of fasting blood glucose (FBG), four blood lipid parameters, liver glycogen, glycogen synthase (GS), glucose-6-phosphatase (G-6-Pase), and aspartate aminotransferase (AST) were measured in each group of rats. A comprehensive evaluation was conducted in combination with histopathological analysis of liver tissues. Results: Compared with the model group, EVO and RUT significantly reduced FBG, alleviated weight loss, improved lipid metabolism, regulated hepatic glucose metabolism (decreased levels of hepatic glycogen, GS, and G-6-Pase), and lowered the level of AST, indicating hepatoprotective effects. Histopathological analysis of the liver showed that EVO and RUT reduced hepatocyte steatosis and inflammatory infiltration, increased hepatic glycogen deposition, and effectively alleviated liver injury. Conclusion: Ergometrine (EVO) and rutabine (RUT) have significant therapeutic potential for liver injury in T2DM rats, lowering blood glucose, regulating blood lipids, modulating hepatic glucose metabolism, and protecting liver tissues, which provides an experimental basis for the development of type 2 antidiabetic drugs.