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三种不同微生物菌剂育苗基质拌菌对烟苗生长的影响
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Abstract:
基质拌菌技术在烟草根茎病害的绿色防控和烟草品质提升中有着广泛的应用。本研究以商洛地区主栽的云烟99品种为研究对象，通过测定烟苗株高等农艺性状和过氧化物酶(POD)等生理指标，探索在基质拌菌技术应用中不同微生物菌剂对烟苗生长的影响差异。研究结果表明：育苗基质经枯草芽孢杆菌、淡紫紫孢菌和哈茨木霉菌处理后，烟苗在农艺性状和叶绿素含量上均表现出正向促进，其中以枯草芽孢杆菌对烟草苗农艺性状影响最显著；三种微生物菌剂对烟苗超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、质膜氧化程度(MDA)含量、渗透调节物质(可溶性蛋白含量和游离脯氨酸含量)生理指标的影响效果存在差异，这可能与三种微生物促进烟草生长的路径与机制不同有关。本研究结果为烟草育苗基质拌菌技术提供了参考。
Substrate mixing technology has a wide range of applications in the green prevention and control of tobacco root and stem diseases and tobacco quality improvement. In this study, the main cultivar in the Shangluo area, Yunyun Tobacco 99, was used as the research object, and the agronomic traits such as plant height and physiological indexes such as peroxidase (POD) were measured to explore the effects of different microbial agents on the growth of tobacco seedlings in the application of the substrate mixing technology. The results of the study showed that after the seedling substrate was treated with Bacillus subtilis, Paecilomyces lilacinus, and Trichoderma harzianum, the agronomic traits and chlorophyll content of tobacco seedlings were positively promoted, among which the effect of Bacillus subtilis on the agronomic traits of tobacco seedlings was the most significant. The differences in the effects of the three microbial agents on the physiological indicators of superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity, plasma membrane oxidation (MDA) content, and osmoregulatory substances (soluble protein content and free cofactor acid content) may be attributed to the differences in the pathways and mechanisms of the three microbial agents in promoting tobacco growth. The results of this study provide a reference for tobacco seedling substrate mixing technology.
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