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不同雪茄烟气浓度暴露对小鼠口腔菌群的影响研究
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Abstract:
目的：本项目计划构建不同雪茄烟气浓度暴露下的小鼠实验模型，探讨雪茄烟气暴露对小鼠口腔菌群的影响。方法：将40只8周龄雄性C57BL/6小鼠随机均分到对照组、雪茄烟气低剂量组、中剂量组以及高剂量组下进行4周饲养，收集小鼠口腔唾液样本，利用16S核糖体RNA (16S ribosomal RNA, 16S rRNA)基因测序技术分析菌群的特征。结果：与对照组相比，不同剂量雪茄干预组小鼠口腔菌群Ace指数以及Shannon指数显著增加(p < 0.05)。在属水平方面，不同组间排名前10的组间差异菌属分别为Bacteroides、Bergeyella、Blautia、Faecalibacterium、Lactobacillus、Megamonas、Muribacter、Prevotella、Rodentibacter、Staphylococcus (p < 0.05)。两两比较结果显示，与对照组相比，中剂量组Acinetobacter菌属丰度特异性显著上升，而高剂量组Blautia、Faecalibacterium特异性显著上升(p < 0.05)。在种水平方面，LEfSe分析结果显示有44个菌种在各组间差异达到显著水平，且不同雪茄剂量组，口腔差异菌种具有剂量特异性(p < 0.05)。结论：雪茄烟气暴露及暴露浓度可影响口腔菌群的微生物组成，同时，不同浓度雪茄对小鼠口腔菌属的影响存在特异性。本项研究有助于深入了解雪茄烟气对口腔健康的影响，为制定相应的预防策略以及改良雪茄加工制造方式提供重要数据基础。
Objective: This project aimed to develop a mouse model subjected to varying concentrations of cigar smoke to investigate the impact of cigar smoke exposure on the oral microbiome of mice. Method: Forty male C57BL/6 mice, aged 8 weeks, were randomly assigned into four groups: control group, low-dose cigar smoke group, medium-dose group, and high-dose group for a feeding period of four weeks. Oral saliva samples were collected from the mice, and 16S ribosomal RNA (16S rRNA) gene sequencing technology was employed to analyze oral microbiome characteristics. Results: Compared to the control group, the Ace and Shannon index of the oral microbiome in all cigar intervention groups exhibited significant increases (p < 0.05). At the genus level, the top ten bacterial genera identified across different groups included Bacteroides, Bergeyella, Blautia, Faecalibacterium, Lactobacillus Megamonas, Muribacter, Prevotella, Rodentibacter and Staphylococcus (p < 0.05). Notably, compared with the control group, the abundance of Acinetobacter significantly increased in the medium-dose group while Blautia and Faecalibacterium markedly decreased in the high-dose group (p < 0.05). Species-level analysis via LEfSe revealed 44 species exhibited significant differences among groups, distinct oral species were dose-specific in different cigar dose groups (p < 0.05). Conclusion: Cigar smoke exposure and exposure concentrations can affect the microbial composition of the oral microbiome. Additionally, different concentrations of cigars exert specific effects on oral bacteria genera in mice. This study enhances the understanding of how cigar smoke influences oral health and provides critical data for developing effective prevention
| [1] | Asakawa, M., Takeshita, T., Furuta, M., Kageyama, S., Takeuchi, K., Hata, J., et al. (2018) Tongue Microbiota and Oral Health Status in Community-Dwelling Elderly Adults. mSphere, 3, e00332-18. https://doi.org/10.1128/msphere.00332-18 |
| [2] | Deo, P. and Deshmukh, R. (2019) Oral Microbiome: Unveiling the Fundamentals. Journal of Oral and Maxillofacial Pathology, 23, 122-128. https://doi.org/10.4103/jomfp.jomfp_304_18 |
| [3] | Wei, A., Li, M., Li, G., Wang, X., Hu, W., Li, Z., et al. (2020) Oral Microbiome and Pancreatic Cancer. World Journal of Gastroenterology, 26, 7679-7692. https://doi.org/10.3748/wjg.v26.i48.7679 |
| [4] | Kato-Kogoe, N., Sakaguchi, S., Kamiya, K., Omori, M., Gu, Y., Ito, Y., et al. (2022) Characterization of Salivary Microbiota in Patients with Atherosclerotic Cardiovascular Disease: A Case-Control Study. Journal of Atherosclerosis and Thrombosis, 29, 403-421. https://doi.org/10.5551/jat.60608 |
| [5] | Jiang, S., Gao, X., Jin, L. and Lo, E. (2016) Salivary Microbiome Diversity in Caries-Free and Caries-Affected Children. International Journal of Molecular Sciences, 17, Article No. 1978. https://doi.org/10.3390/ijms17121978 |
| [6] | Capurso, G. and Lahner, E. (2017) The Interaction between Smoking, Alcohol and the Gut Microbiome. Best Practice & Research Clinical Gastroenterology, 31, 579-588. https://doi.org/10.1016/j.bpg.2017.10.006 |
| [7] | Macgregor, I.D. (1989) Effects of Smoking on Oral Ecology. A Review of the Literature. Clinical Preventive Dentistry, 11, 3-7. |
| [8] | Brook, I. (2011) The Impact of Smoking on Oral and Nasopharyngeal Bacterial Flora. Journal of Dental Research, 90, 704-710. https://doi.org/10.1177/0022034510391794 |
| [9] | Tan, J., Lamont, G.J. and Scott, D.A. (2024) Tobacco‐Enhanced Biofilm Formation by Porphyromonas gingivalis and Other Oral Microbes. Molecular Oral Microbiology, 39, 270-290. https://doi.org/10.1111/omi.12450 |
| [10] | Mason, M.R., Preshaw, P.M., Nagaraja, H.N., Dabdoub, S.M., Rahman, A. and Kumar, P.S. (2014) The Subgingival Microbiome of Clinically Healthy Current and Never Smokers. The ISME Journal, 9, 268-272. https://doi.org/10.1038/ismej.2014.114 |
| [11] | Suzuki, N., Nakano, Y., Yoneda, M., Hirofuji, T. and Hanioka, T. (2021) The Effects of Cigarette Smoking on the Salivary and Tongue Microbiome. Clinical and Experimental Dental Research, 8, 449-456. https://doi.org/10.1002/cre2.489 |
| [12] | Hutcherson, J.A., Gogenini, H., Lamont, G.J., Miller, D.P., Nowakowska, Z., Lasica, A.M., et al. (2019) Porphyromonas gingivalis Genes Conferring Fitness in a Tobacco‐rich Environment. Molecular Oral Microbiology, 35, 10-18. https://doi.org/10.1111/omi.12273 |
| [13] | Botteri, E., Borroni, E., Sloan, E.K., Bagnardi, V., Bosetti, C., Peveri, G., et al. (2020) Smoking and Colorectal Cancer Risk, Overall and by Molecular Subtypes: A Meta-Analysis. American Journal of Gastroenterology, 115, 1940-1949. https://doi.org/10.14309/ajg.0000000000000803 |
| [14] | Khoramdad, M., Vahedian‐azimi, A., Karimi, L., Rahimi‐Bashar, F., Amini, H. and Sahebkar, A. (2019) Association between Passive Smoking and Cardiovascular Disease: A Systematic Review and Meta‐analysis. IUBMB Life, 72, 677-686. https://doi.org/10.1002/iub.2207 |
| [15] | Huang, Q., Wu, X., Zhou, X., Sun, Z., Shen, J., Kong, M., et al. (2023) Association of Cigarette Smoking with Oral Bacterial Microbiota and Cardiometabolic Health in Chinese Adults. BMC Microbiology, 23, Article No. 346. https://doi.org/10.1186/s12866-023-03061-y |
| [16] | Antonello, G., Blostein, F., Bhaumik, D., Davis, E., Gögele, M., Melotti, R., et al. (2023) Smoking and Salivary Microbiota: A Cross-Sectional Analysis of an Italian Alpine Population. Scientific Reports, 13, Article No. 18904. https://doi.org/10.1038/s41598-023-42474-7 |
| [17] | Yu, G., Phillips, S., Gail, M.H., Goedert, J.J., Humphrys, M.S., Ravel, J., et al. (2017) The Effect of Cigarette Smoking on the Oral and Nasal Microbiota. Microbiome, 5, Article No. 3. https://doi.org/10.1186/s40168-016-0226-6 |
| [18] | Al Bataineh, M.T., Dash, N.R., Elkhazendar, M., Alnusairat, D.M.H., Darwish, I.M.I., Al-Hajjaj, M.S., et al. (2020) Revealing Oral Microbiota Composition and Functionality Associated with Heavy Cigarette Smoking. Journal of Translational Medicine, 18, Article No. 421. https://doi.org/10.1186/s12967-020-02579-3 |
| [19] | Jia, Y., Liao, Y., He, Y., Zheng, M., Tong, X., Xue, W., et al. (2021) Association between Oral Microbiota and Cigarette Smoking in the Chinese Population. Frontiers in Cellular and Infection Microbiology, 11, Article ID: 658203. https://doi.org/10.3389/fcimb.2021.658203 |
| [20] | Wu, Z., Han, Y., Caporaso, J.G., Bokulich, N., Mohamadkhani, A., Moayyedkazemi, A., et al. (2021) Cigarette Smoking and Opium Use in Relation to the Oral Microbiota in Iran. Microbiology Spectrum, 9, e00138-21. https://doi.org/10.1128/spectrum.00138-21 |
| [21] | Wu, J., Peters, B.A., Dominianni, C., Zhang, Y., Pei, Z., Yang, L., et al. (2016) Cigarette Smoking and the Oral Microbiome in a Large Study of American Adults. The ISME Journal, 10, 2435-2446. https://doi.org/10.1038/ismej.2016.37 |
| [22] | Sato, N., Kakuta, M., Uchino, E., Hasegawa, T., Kojima, R., Kobayashi, W., et al. (2020) The Relationship between Cigarette Smoking and the Tongue Microbiome in an East Asian Population. Journal of Oral Microbiology, 12, Article ID: 1742527. https://doi.org/10.1080/20002297.2020.1742527 |
| [23] | National Cancer Institute (2024) Cigar Smoking and Cancer. https://www.cancer.gov/about-cancer/causes-prevention/risk/tobacco/cigars-fact-sheet#r3 |
| [24] | Kong, G., Mayer, M.E., Barrington-Trimis, J.L., McConnell, R., Leventhal, A.M. and Krishnan-Sarin, S. (2019) Longitudinal Associations between Use and Co-Use of Cigars and Cigarettes: A Pooled Analysis of Three Adolescent Cohorts. Drug and Alcohol Dependence, 201, 45-48. https://doi.org/10.1016/j.drugalcdep.2019.03.022 |
| [25] | Kumar, P.S., Matthews, C.R., Joshi, V., de Jager, M. and Aspiras, M. (2011) Tobacco Smoking Affects Bacterial Acquisition and Colonization in Oral Biofilms. Infection and Immunity, 79, 4730-4738. https://doi.org/10.1128/iai.05371-11 |
| [26] | Güntsch, A., Erler, M., Preshaw, P.M., Sigusch, B.W., Klinger, G. and Glockmann, E. (2006) Effect of Smoking on Crevicular Polymorphonuclear Neutrophil Function in Periodontally Healthy Subjects. Journal of Periodontal Research, 41, 184-188. https://doi.org/10.1111/j.1600-0765.2005.00852.x |
| [27] | Yang, Y., Zheng, W., Cai, Q., Shrubsole, M.J., Pei, Z., Brucker, R., et al. (2019) Cigarette Smoking and Oral Microbiota in Low-Income and African-American Populations. Journal of Epidemiology and Community Health, 73, 1108-1115. https://doi.org/10.1136/jech-2019-212474 |
| [28] | Yan, S., Ma, Z., Jiao, M., Wang, Y., Li, A. and Ding, S. (2021) Effects of Smoking on Inflammatory Markers in a Healthy Population as Analyzed via the Gut Microbiota. Frontiers in Cellular and Infection Microbiology, 11, Article ID: 633242. https://doi.org/10.3389/fcimb.2021.633242 |
| [29] | Maki, K.A., Ganesan, S.M., Meeks, B., Farmer, N., Kazmi, N., Barb, J.J., et al. (2022) The Role of the Oral Microbiome in Smoking-Related Cardiovascular Risk: A Review of the Literature Exploring Mechanisms and Pathways. Journal of Translational Medicine, 20, Article No. 584. https://doi.org/10.1186/s12967-022-03785-x |
| [30] | Bai, X., Wei, H., Liu, W., Coker, O.O., Gou, H., Liu, C., et al. (2022) Cigarette Smoke Promotes Colorectal Cancer through Modulation of Gut Microbiota and Related Metabolites. Gut, 71, 2439-2450. https://doi.org/10.1136/gutjnl-2021-325021 |
| [31] | Vasconcelos, F.M., Silva, H.L.A., Poso, S.M.V., Barroso, M.V., Lanzetti, M., Rocha, R.S., et al. (2019) Probiotic Prato Cheese Attenuates Cigarette Smoke-Induced Injuries in Mice. Food Research International, 123, 697-703. https://doi.org/10.1016/j.foodres.2019.06.001 |
| [32] | Chen, S., Xin, J., Gu, D., Li, H., Zheng, R., Li, S., et al. (2024) Smoking-Related lactobacillus and Immune Cell Infiltration in Colorectal Cancer: Evidence from a Population-Based Study. Gut. https://doi.org/10.1136/gutjnl-2023-331865 |
| [33] | Yang, H., Xiu, W., Liu, J., Yang, Y., Zhang, Y., Zheng, Y., et al. (2022) Characteristics of the Intestinal Microorganisms in Middle-Aged and Elderly Patients: Effects of Smoking. ACS Omega, 7, 1628-1638. https://doi.org/10.1021/acsomega.1c02120 |
| [34] | Antinozzi, M., Giffi, M., Sini, N., Gallè, F., Valeriani, F., De Vito, C., et al. (2022) Cigarette Smoking and Human Gut Microbiota in Healthy Adults: A Systematic Review. Biomedicines, 10, Article No. 510. https://doi.org/10.3390/biomedicines10020510 |
| [35] | Sharma, G., Garg, N., Hasan, S. and Shirodkar, S. (2022) Prevotella: An Insight into Its Characteristics and Associated Virulence Factors. Microbial Pathogenesis, 169, Article ID: 105673. https://doi.org/10.1016/j.micpath.2022.105673 |
| [36] | Miller, D.P., Wang, Q., Weinberg, A. and Lamont, R.J. (2018) Transcriptome Analysis of Porphyromonas gingivalis and Acinetobacter baumannii in Polymicrobial Communities. Molecular Oral Microbiology, 33, 364-377. https://doi.org/10.1111/omi.12238 |
