The aim of this study was to evaluate in vivo the effect of the aqueous extract of Sarcocephaluslatifolius root powder on haemostasis parameters and lipid profile in male and female Wistar rats. The plant material consisted exclusively of Sarcocephaluslatifolius roots harvested in Porto-Novo, southern Benin and certified at the Benin National Herbarium under number YH 790/HNB. The animals were divided into four groups of three rats and gavaged for 14 days. The control rats were given distilled water orally, while the test batches were given 100, 200 and 300 mg/Kg of BW extract respectively. Blood samples were taken on days 7 and 14 of treatment and used to explore the intrinsic and extrinsic pathways of haemostasis. These included parameters such as bleeding time, prothrombin time, activated partial thromboplastin time, thrombin time and platelet count. Lipid parameters were also explored. Parameters such as total cholesterol, HDL cholesterol, LDL cholesterol and triglycerides were studied. Data on days D0, D7 and D14 for each haemostasis parameter and lipid profile were expressed as the mean plus or minus the standard error for each batch of animals. The data on day D0 were compared with those on days D7 and D14 to check whether there was a significant difference between the data obtained. The difference between D0 and D14 was tested using the parametric paired two-sample test using R Studio software and Graphpad Prism 9.5.1 software (733). A significant dose-dependent decrease in bleeding time, prothrombin time, activated partial thromboplastin and thrombin was recorded, with the exception of the platelet count. As regards lipid parameters, there was a significant dose-dependent reduction in LDL cholesterol and triglycerides, and an increase in HDL cholesterol. The results confirm that the roots of Sarcocephaluslatifolius accelerate the arrest of bleeding, thanks to its ability to stimulate the formation of platelet clots and improve the lipid profile of the animals by eliminating bad cholesterol.
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