Background: Oxidative stress is implicated in liver disease pathogenesis. Liver injuries are traditionally treated with stem bark from Spondiasmombin. In our previous study, this plant demonstrated promising invitro antioxidant activities. Objective: The purpose of this study was to evaluate the antioxidant and hepatocurative activity of methanolic extract of S.mombin (MESPM) through invivo studies. Methods: Thirty rats were divided into five groups containing six rats each. Group I was the normal control, group II was the negative control and the other groups were experimental groups. Rats in groups II - V received, firstly, 2 mL/kg/day of carbon tetrachloride (CCl4) intraperitoneally, for three days to induce oxidative stress and hepatotoxicity. On the following day, groups IV and V were administered MESPM orally for eleven consecutive days, respectively, at 200 and 400 mg/kg/day. Silymarin (50 mg/kg) was used as a standard (group II: positive control). Serum biochemical parameters and invivo antioxidant activity were measured using standard procedures. Gross and histopathological studies were also performed. Results: CCl4 induced oxidative stress was manifested by an increase in TBARS level as well as a decrease in FRAP level and DPPH radicals scavenging percentage compared to normal control (P < 0.001). Furthermore, CCl4 hepatotoxicity caused significant increases in ALT, AST, ALP, bilirubin levels, with a decline in total proteins (P < 0.001). However, as a result of treatment with silymarin or MESPM (400 mg/kg), enzyme activities, bilirubina and total protein levels, were significantly reversed. Oxidative stress parameters were also restored. The macroscopic and histopathological changes of hepatocytes against CCl4 confirmed that MESPM was effective. Conclusion: The results confirm MESPM’s traditional use as a hepatocurative agent, probably due to its antioxidant properties.
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