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痰热清注射液治疗重症肺炎的网络药理分析
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Abstract:
目的：通过网络药理学策略，评价痰热清注射液治重症肺炎的临床疗效，探索其干预机制。方法：通过检索数据库及相关文献查找痰热清注射液的潜在的活性化合物及靶点；以“severe pneumonia”、“severe case pneumonia”为关键词分别检索DrugBank、GeneCards、TTD、OMIM和PharmGKB疾病数据库得到与重症肺炎相关的疾病靶基因；通过Cytoscape 3.9.2软件构建“中药–潜在活性化合物–重症肺炎潜在靶点”网络，以及重症肺炎靶点与痰热清注射液活性化合物调控的交集靶基构建PPI网络，并对核心靶基因进行Gene Ontology (GO)功能富集分析及Kyoto Encyclopedia of Genes and Genomes (KEGG)通路富集分析，初步探讨痰热清注射液干预重症肺炎的潜在机制。结果：检索得到痰热清注射液163个药物活性成分和414个药物靶标，其中药物与疾病共同靶标291个，筛选出痰热清注射液治疗重症肺炎的关键的活性化合物，主要有槲皮素(quercetin)，芹黄素(apigenin)，熊果酸(ursolic acid)，木草素(luteolin)，山奈酚(kaempferol)，汉黄芩素(wogonin) GO功能富集分析痰热清注射液参与调控positive regulation of gene expression、positive regulation of pri-miRNA transcription from RNA polymerase II promoter等生物过程(BP)，transcription factor complex等细胞功能(CC)，以及enzyme binding、identical protein binding等的分子功能(MF)；KEGG通路富集显示痰热清注射液可以调控肿瘤坏死因子(Tumor Necrosis Factor, TNF)信号通路，以及白介素-17 (IL-17)信号通路等与重症肺炎相关的疾病通路。结论：通过网络药理学研究发现，痰热清注射液干预重症肺炎是多成分、多靶标共同作用的综合性结果，其主要通过干预炎症反应途径达到治疗重症肺炎的目的。
Objective: To evaluate the clinical efficacy of Tanreqing injection in the treatment of severe pneumonia and explore its intervention mechanism through network pharmacological strategy. Methods: The potential active compounds and targets of Tanreqing injection were found by searching database and related literature. “Severe pneumonia” and “severe case pneumonia” were searched in DrugBank, GeneCards, TTD, OMIM and PharmGKB disease databases to obtain the target genes associated with severe pneumonia. The network of “Traditional Chinese Medicine - Potentially active Compound - Potential Target of severe pneumonia” was constructed by Cytoscape 3.9.2 software, and the PPI network was constructed based on the intersection target of severe pneumonia target and the active compound of Tanreqing injection. The Gene Ontology (GO) function enrichment analysis and Kyoto Encyclopedia of Genes and Genes (KEGG) pathway enrichment analysis were also conducted, and the potential mechanism of Tanreqing injection in the intervention of severe pneumonia was preliminologically discussed. Result: A total of 163 active ingredients and 414 drug targets of Tanreqing injection were retrieved, 291 of which were common targets of drugs and diseases. The key active compounds of Tanreqing injection in the treatment of severe pneumonia were selected, including quercetin, apigenin, ursolic acid,
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