The objective of this study was to examine the sorghum preservation techniques in response to climate variability and to evaluate the prevalence of aflatoxin B1 in selected sorghum stocks in Burkina Faso. The cross-sectional study was conducted over a period of six months, from October 2020 to January 2021. A questionnaire was administered to 450 sorghum farmers in order to ascertain their knowledge and practices with regard to conservation techniques. A total of 23 farmers’ stocks were sampled for the determination of aflatoxin B1 using the Enzyme-Linked Immunosorbent Assay (ELISA) method and for the assessment of moisture levels using the Association of Official Agricultural Chemists (AOAC) method. A total of five distinct preservation techniques were identified. The most frequently utilized techniques were straw loft (49.2%) as a storage structure, pallets (36.69%) as the structure’s internal management, plastic bags (40.42%) as packaging material, panicles (64.3%) as a form of storage, and chemical products (15.22%) as preservatives. Three biotic constraints were identified: insect (51.18%), rodent (30.14%), and mold (15.38%). Three significant abiotic constraints were identified: humidity (44.6%), lack of hygiene (15.2%), and grain immaturity (10.2%). A comparative analysis reveals that the majority of these techniques and constraints exhibit notable differences between climatic zones, largely due to the influence of climatic variability. The aflatoxin B1 level exhibited considerable variation, ranging from 0 μg/kg to 2.07 ± 0.08 μg/kg DM. Of the analyzed stocks, 38% were found to be contaminated. Abiotic and biotic factors exert influence on sorghum stocks. Chemical agents are employed for their protection, and contamination by aflatoxin B1 is a further issue. However, the levels of contamination observed are not cause for concern.
References
[1]
Rumler, R., Bender, D. and Schönlechner, R. (2022) Sorghum and Its Potential for the Western Diet. Journal of Cereal Science, 104, Article 103425. https://doi.org/10.1016/j.jcs.2022.103425
[2]
Stamenković, O.S., Siliveru, K., Veljković, V.B., Banković-Ilić, I.B., Tasić, M.B., Ciampitti, I.A., et al. (2020) Production of Biofuels from Sorghum. Renewable and Sustainable Energy Reviews, 124, Article 109769. https://doi.org/10.1016/j.rser.2020.109769
[3]
Nciizah, A.D., Rapetsoa, M.C., Wakindiki, I.I. and Zerizghy, M.G. (2020) Micronutrient Seed Priming Improves Maize (Zea mays) Early Seedling Growth in a Micronutrient Deficient Soil. Heliyon, 6, e04766. https://doi.org/10.1016/j.heliyon.2020.e04766
[4]
Majid, A., Lakshmikanth, M., Lokanath, N.K. and Poornima Priyadarshini, C.G. (2022) Generation, Characterization and Molecular Binding Mechanism of Novel Dipeptidyl Peptidase-4 Inhibitory Peptides from Sorghum bicolor Seed Protein. Food Chemistry, 369, Article 130888. https://doi.org/10.1016/j.foodchem.2021.130888
[5]
Ulian, T., Diazgranados, M., Pironon, S., Padulosi, S., Liu, U., Davies, L., et al. (2020) Unlocking Plant Resources to Support Food Security and Promote Sustainable Agriculture. PLANTS, PEOPLE, PLANET, 2, 421-445. https://doi.org/10.1002/ppp3.10145
[6]
FAO (2016) Legumineuses Des graines nutritives pour un avenir durable.
[7]
Ndlovu, M.S. and Demlie, M. (2020) Assessment of Meteorological Drought and Wet Conditions Using Two Drought Indices across Kwazulu-Natal Province, South Africa. Atmosphere, 11, Article 623. https://doi.org/10.3390/atmos11060623
[8]
MARAH/EPA (2021) Rapport definitifs sur la campagne agricole 2020/2021 du Burkina Faso.
[9]
Zia-Ur-Rehman (2006) Storage Effects on Nutritional Quality of Commonly Consumed Cereals. Food Chemistry, 95, 53-57. https://doi.org/10.1016/j.foodchem.2004.12.017
[10]
Waongo, A., Yamkoulga, M., Dabire-Binso, C., Ba, M. and Sanon, A. (2013) Conservation post-récolte des céréales en zone sud-soudanienne du Burkina Faso: Perception paysanne et évaluation des stocks. International Journal of Biological and Chemical Sciences, 7, 1157-1167. https://doi.org/10.4314/ijbcs.v7i3.22
[11]
Woodward, A.J. and Samet, J.M. (2018) Climate Change, Hurricanes, and Health. American Journal of Public Health, 108, 33-35. https://doi.org/10.2105/ajph.2017.304197
[12]
Udomkun, P., Tirawattanawanich, C., Ilukor, J., Sridonpai, P., Njukwe, E., Nimbona, P., et al. (2019) Promoting the Use of Locally Produced Crops in Making Cereal-Legume-Based Composite Flours: An Assessment of Nutrient, Antinutrient, Mineral Molar Ratios, and Aflatoxin Content. Food Chemistry, 286, 651-658. https://doi.org/10.1016/j.foodchem.2019.02.055
[13]
Deligeorgakis, C., Magro, C., Skendi, A., Gebrehiwot, H.H., Valdramidis, V. and Papageorgiou, M. (2023) Fungal and Toxin Contaminants in Cereal Grains and Flours: Systematic Review and Meta-Analysis. Foods, 12, Article 4328. https://doi.org/10.3390/foods12234328
[14]
Kumar, D. and Kalita, P. (2017) Reducing Postharvest Losses during Storage of Grain Crops to Strengthen Food Security in Developing Countries. Foods, 6, Article 8. https://doi.org/10.3390/foods6010008
[15]
Min, L., Fink-Gremmels, J., Li, D., Tong, X., Tang, J., Nan, X., et al. (2021) An Overview of Aflatoxin B1 Biotransformation and Aflatoxin M1 Secretion in Lactating Dairy Cows. Animal Nutrition, 7, 42-48. https://doi.org/10.1016/j.aninu.2020.11.002
[16]
Xue, K.S., Tang, L., Shen, C.L., Pollock, B.H., Guerra, F., Phillips, T.D., et al. (2021) Increase in Aflatoxin Exposure in Two Populations Residing in East and West Texas, United States. International Journal of Hygiene and Environmental Health, 231, Article 113662. https://doi.org/10.1016/j.ijheh.2020.113662
[17]
Zingales, V., Taroncher, M., Martino, P.A., Ruiz, M. and Caloni, F. (2022) Climate Change and Effects on Molds and Mycotoxins. Toxins, 14, Article 445. https://doi.org/10.3390/toxins14070445
[18]
Lwanga, S. and Lemeshow, S. (1991) Sample Size Determination in Health Studies: A Practical Manual. World Health Organization.
[19]
AOAC (1990) Official Method of Analysis. 15th Edition, AOAC International.
[20]
Abbas, H.K., Zablotowicz, R.M., Weaver, M.A., Horn, B.W., Xie, W. and Shier, W.T. (2004) Comparison of Cultural and Analytical Methods for Determination of Aflatoxin Production by Mississippi Delta Aspergillus Isolates. Canadian Journal of Microbiology, 50, 193-199. https://doi.org/10.1139/w04-006
[21]
Raa, F., Cisse, M., Sarr, F., Brabet, C. and Dieme, E. (2020) Cultural Practices and Post-Harvest Management of Sorghum in Senegal. International Journal of Biological and Chemical Sciences, 14, 1001-1013.
[22]
Savadogo, S., Sambare, O., Sereme, A. and Thiombiano, A. (2016) Méthodes traditionnelles de lutte contre les insectes et les tiques chez les Mossé au Burkina Faso. Journal of Applied Biosciences, 105, Article 10120. https://doi.org/10.4314/jab.v105i1.9
[23]
Nanfack, F., Dongmo, Y. and Fogang, M. (2015) Les insectes impliqués dans les pertes post-récolte des céréales au Cameroun: Méthodes actuelles de lutte et perspectives offertes par la transgénèse. International Journal of Biological and Chemical Sciences, 9, 1630-1643. https://doi.org/10.4314/ijbcs.v9i3.42
[24]
Guèye, M.T., Seck, D. and Wathelet, J.-P. (2011) Lutte contre les ravageurs des stocks de céréales et de légumineuses au Sénégal et en Afrique occidentale: Synthèse bibli-ographique. Biotechnologie, Agronomie, Société et Environnement, 15, 183-194.
[25]
Ashamo, M. (2006) Relative Susceptibility of Some Local and Elite Rice Varieties to the Rice Weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae). Journal of Food Agriculture and Environment, 4, 249-252.
[26]
Ndomo, A.F., Tapondjou, A.L., Fernand, T. and Tchouanguep, F. (2009) Evaluation des propriétés insecticides des feuilles de Callistemon viminalis (Myrtaceae) contre les adultes d’Aconthoscelides obtectus (Say) (Coleoptera; Bruchidae). Tropicultura, 27, 137-143. https://www.researchgate.net/publication/45266596
[27]
Bradford, K.J., Dahal, P., Van Asbrouck, J., Kunusoth, K., Bello, P., Thompson, J., et al. (2018) The Dry Chain: Reducing Postharvest Losses and Improving Food Safety in Humid Climates. Trends in Food Science & Technology, 71, 84-93. https://doi.org/10.1016/j.tifs.2017.11.002
[28]
Akinola, S.A., Ateba, C.N. and Mwanza, M. (2021) Behaviour of Aspergillus Parasiticus in Aflatoxin Production as Influenced by Storage Parameters Using Response Surface Methodology Approach. International Journal of Food Microbiology, 357, Article 109369. https://doi.org/10.1016/j.ijfoodmicro.2021.109369
[29]
Molnár, K., Rácz, C., Dövényi-Nagy, T., Bakó, K., Pusztahelyi, T., Kovács, S., et al. (2023) The Effect of Environmental Factors on Mould Counts and AFB1 Toxin Production by Aspergillus flavus in Maize. Toxins, 15, Article 227. https://doi.org/10.3390/toxins15030227
[30]
IARC (2015) Mycotoxin Control in Lowand Middle Income Countries. IARC (International Agency for Research on Cancer).
[31]
Obonyo, M.A. and Salano, E.N. (2018) Perennial and Seasonal Contamination of Maize by Aflatoxins in Eastern Kenya. International Journal of Food Contamination, 5, Article No. 6. https://doi.org/10.1186/s40550-018-0069-y
[32]
Wu, F. (2015) Global Impacts of Aflatoxin in Maize: Trade and Human Health. World Mycotoxin Journal, 8, 137-142. https://doi.org/10.3920/wmj2014.1737
[33]
Liu, W., Wu, Y., Wang, C., Li, H.C., Wang, T., Liao, C.Y., et al. (2010) Impact of Silver Nanoparticles on Human Cells: Effect of Particle Size. Nanotoxicology, 4, 319-330. https://doi.org/10.3109/17435390.2010.483745
[34]
AFSSA (2009) Évaluation des risques liés à la présence de mycotoxines dans les chaînes alimentaires humaine et animale. Rapport Final.
[35]
EFSA (2013) Aflatoxins (Sum of B1, B2, G1, G2) in Cereals and Cereal-Derived Food Products 1. Technical Report, European Food Safety Authority (EFSA).
[36]
Huerta-Treviño, A. Sánchez, JED-A, E. Heredia, N. and García, S. (2016) Occurrence of Mycootoxins in Alfalfa (Medicago sativa L.), Sorghum [Sorghum bicolor (L.) Moench], and Grass (Cenchrus ciliaris L.) Retailed in the State of Nuevo León. México. Agrociencia, 50, 825-836.
[37]
Ratnavathi, C.V., Komala, V.V. and Chavan, U.D. (2016) Mycotoxin Contamination in Sorghum. In: Ratnavathi, C.V., Patil, J.V. and Chavan, U.D., Eds., Sorghum Biochemistry:An Industrial Perspective, Academic Press, 107-180. https://doi.org/10.1016/b978-0-12-803157-5.00003-4
[38]
Kortei, N.K., Annan, T., Dzikunoo, J. and Agbetiameh, D. (2022) Exposure Assessment and Risk Characterization of Aflatoxins Intake through Consumption of Maize (Zea mays) in Different Age Populations in the Volta Region of Ghana. International Journal of Food Contamination, 9, Article No. 13. https://doi.org/10.1186/s40550-022-00099-0
[39]
Leiva, A., Méndez, G., Rodríguez, C., Molina, A. and Granados-Chinchilla, F. (2019) Chemical Assessment of Mycotoxin Contaminants and Veterinary Residues in Costa Rican Animal Feed. International Journal of Food Contamination, 6, Article No. 5. https://doi.org/10.1186/s40550-019-0075-8
[40]
Sultana, B., Naseer, R. and Nigam, P. (2015) Utilization of Agro-Wastes to Inhibit Aflatoxins Synthesis by Aspergillus parasiticus: A Biotreatment of Three Cereals for Safe Long-Term Storage. Bioresource Technology, 197, 443-450. https://doi.org/10.1016/j.biortech.2015.08.113
[41]
Sserumaga, J.P., Ortega-Beltran, A., Wagacha, J.M., Mutegi, C.K. and Bandyopadhyay, R. (2020) Aflatoxin-Producing Fungi Associated with Pre-Harvest Maize Contamination in Uganda. International Journal of Food Microbiology, 313, Article 108376. https://doi.org/10.1016/j.ijfoodmicro.2019.108376
[42]
Norlia, M., Jinap, S., Nor-Khaizura, M.A.R., Radu, S., Samsudin, N.I.P. and Azri, F.A. (2019) Aspergillus Section Flavi and Aflatoxins: Occurrence, Detection, and Identification in Raw Peanuts and Peanut-Based Products along the Supply Chain. Frontiers in Microbiology, 10, Article 2602. https://doi.org/10.3389/fmicb.2019.02602
