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鼠李糖乳杆菌GKLC1减少香烟烟雾引起的过敏性炎症
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Abstract:
香烟烟雾(cigarette smoke, CS)中含有多种有害物刺激物，容易经呼吸道吸入引起过敏性炎症反应，特别是对已具过敏体质者。本研究评估鼠李糖乳杆菌(Lactobacillus rhamnosus)菌株GKLC1对降低香烟烟雾引起之过敏性炎症反应的效果。实验采用7周龄之Balb/c雄性小鼠随机分成过敏对照组(OVA + CS)与菌株GKLC1组(GKLC1)，每组6只。所有试验小鼠于试验第0、12天进行腹腔注射卵白蛋白抗原(Ovalbumin, OVA, 500 ug/ml)，并于试验第17日至23日以2% OVA进行喷雾致敏，诱发过敏体质。试验第24日至54日，小鼠每日暴露10分钟之香烟烟雾加剧过敏性炎症反应。比较过敏性小鼠于CS刺激期间每日补充菌株GKLC1 (4.5 mg/day)与否，对于体内发炎相关指标之影响。结果发现，菌株GKLC1组之小鼠血清中内毒素显著低于过敏对照组(p < 0.001)，且肝脏中的细胞激素IFN-γ、TNF-α、IL-4、IL-5分泌量皆显著减少，显示菌株GKLC1补充有助于降低香烟烟雾引起之过敏性发炎反应。
Cigarette smoke (CS) contains various harmful irritants, which can easily be inhaled through the respiratory tract, causing allergic inflammatory reactions, especially in individuals with pre-existing allergic conditions. This study assessed the effectiveness of the Lactobacillus rhamnosus strain GKLC1 in reducing allergic inflammatory reactions caused by CS. Male Balb/c mice at 7 weeks of age were randomly divided into an allergic control group (OVA + CS) and a GKLC1 strain group (GKLC1), with 6 mice in each group. All experimental mice were intraperitoneally injected with ovalbumin antigen (OVA, 500 ug/ml) on days 0 and 12, and sensitized with 2% OVA inhalation from days 17 to 23 to induce allergic predisposition. From day 24 to day 54 of the experiment, mice were exposed to CS for 10 minutes daily to exacerbate allergic inflammatory reactions. The impact of daily supplementation of the strain GKLC1 (4.5 mg/day) on inflammatory markers in allergic mice during CS stimulation was compared. The results showed that the serum endotoxin levels in the GKLC1 group were significantly lower than those in the allergic control group (p < 0.001). Additionally, the secretion of cytokines IFN-γ, TNF-α, IL-4, and IL-5 in the liver was significantly reduced in the GKLC1 group, indicating that supplementation with the strain GKLC1 contributed to reducing allergic inflammatory reactions caused by cigarette smoke.
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