Rotavirus is the leading cause of acute gastroenteritis and responsible for 20% of death in children under 5 years of age approximately 111 million episodes of gastroenteritis due to Rotavirus in under 5 children, which result in 25 million visits to clinic, 2 million hospitalization and 352,000 - 592,000 deaths. Children in the poorest countries account 82% of rotaviruses death. Diarrhea disease are major cause of death and disease among children under five years, a child on average suffers 2 to 3 attacks of diarrhea every year. Stools samples were collected from 196 children suffering from diarrhea and were tested for rotaviruses by immune chromatography test antigen (ICT-Ag), ELISA and RT PCR The data were analyzed using statistical package for the Social Sciences (SPSS). The results showed that prevalence of rotavirus was 26/196 (13.3%) by ICT Ag and ELISA and confirmed by RT-PC R. 87.8% of the children were vaccinated by Rota vaccine, 70% of their mothers not using soap for cleaning the children and thought diarrhea not infectious disease and believed it was caused by teething. In conclusion there was a decrease in prevalence of diarrhea by Rotavirus due to insertion of Rota vaccine in routine vaccination, rotavirus infection mostly occurs in poor family that not able to provide safe water and due to poor sanitation and low education of mother beside that some children suffered from malnutrition, so it recommended to incorporate ELISA as a diagnostic tool in routine diagnosis of rotavirus among children suffered from gastroenteritis.
References
[1]
World Health Organization (2009) Global Rotavirus Information and Surveillance Reporting Period: January through December 2008.
[2]
Sherchand, J.B., Yokoo, M., Sherchand, O., Pant, A.R. and Nakogomi, O. (1970) Burden of Enteropathogens Associated Diarrheal Diseases in Children Hospital, Nepal. ScientificWorld, 7, 71-75. https://doi.org/10.3126/sw.v7i7.3830
Knipe, D.M., David, M., Howley Peter, M. and Chanock, R.M. (2007) Rotaviruses. In: Knipe, D.M. and Chanock, R.M., Eds., Fields Virology, 5th Edition, Lippincott Williams & Wilkins, 1918-1963.
[5]
Desselberger, U. and Gray, J. (2009) Viruses Associated with Acute Diarrheal Disease. In: Zukerman, A.J., Banatvala, J.E., Schoub, B.D., Griffith, P.D. and Mortimer, P., Ed., Principles and Practice of Clinical Virology, 6th Edition, John Wiley & Sons, 249-270.
[6]
Parashar, U.D., Gibson, C.J., Bresee, J.S. and Glass, R.I. (2006) Rotavirus and Severe Childhood Diarrhea. EmergingInfectiousDiseases, 12, 304-306. https://doi.org/10.3201/eid1202.050006
[7]
Gray, J., Vesikari, T., Damme, P.V., Giaquinto, C., Mrukowicz, J., et al. (2008) Rotavirus. Journal of Pediatric Gastroenterology and Nutrition, 46, 24-31. https://doi.org/10.1097/mpg.0b013e31816f78ee
[8]
Brooks, G.F., Caaroll, K.C., Butel, J.S., Morse, S.A. and Metzger, T.A. (2010) Reoviruses, Rotaviruses, and Caliciviruses. In: Carroll, K.C., Hobden, J.A., Miller, S., Morse, S.A., Mietzner, T.A., Detrick, B., Mitchell, T.G., McKerrow, J.H. and Sakanari, J.A., Eds., Jawetz, Melnick, & Adelberg’s Medical Microbiology, 25th Edition, McGraw Hill Medical, 508-512.
[9]
Ling, J.M. and Cheng, A.F. (1993) Infectious Diarrhea in Hong Kong. The Journal of Tropical Medicine and Hygiene, 96, 107-112.
[10]
Abu Elamreen, F.H., Abed, A.A. and Sharif, F.A (2006) Rotavirus in Infants and Young Children with Acute Gastroenteritis in Gaza. Palestine Sands of Al-Quds Medicine, 2, 11-17.
[11]
World Health Organization (2009) Manual of Rotavirus Detection and Characterization Methods, 1-114.
[12]
Ramig, R.F. (2004) Pathogenesis of Intestinal and Systemic Rotavirus Infection. Journal of Virology, 78, 10213-10220. https://doi.org/10.1128/jvi.78.19.10213-10220.2004
[13]
World Health Organization (2010) Global Rotavirus Information and Surveillance Bulletin.