L-tryptophan is an essential amino acid for human health. Nanofibrillated cellulose (NFC) from marram grass (Ammophilaarenaria) extracted from plants harvested in the center of Tunisia was used for the first time for the modification of a glassy carbon electrode (GCE), for the sensitive detection of L-tryptophan (Trp). After spectroscopic and morphological characterization of the extracted NFC, the GC electrode modification was monitored through cyclic voltammetry. The NFC-modified electrode exhibited good analytical performance in detecting Trp with a wide linear range between 7.5 × 10？4 mM and 10？2 mM, a detection limit of 0.2 μM, and a high sensitivity of 140.0 μA？mM？1. Additionally, the NFC/GCE showed a good reproducibility, good selectivity versus other amino acids, uric acid, ascorbic acid, and good applicability to the detection of Trp in urine samples.
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