草菇液体菌种培养条件的对比试验
Comparative Experiment on the Cultivation Conditions of Volvariella volvacea Liquid Strains

为了筛选合适的草菇液体菌种培养条件，本实验设计三个配方进行草菇液体菌种摇床培养对比试验，筛选出适宜的培养基配方，即马铃薯50 g，葡萄糖20 g，蛋白胨1.5 g，KH₂PO₄ 1 g，MgSO₄ 0.5 g，维生素B₁ 10 mg，水1000 mL，pH自然。在此基础上设计摇床转速、维生素B₁添加浓度、培养基装瓶量、摇前静置时间等四个因子，进行草菇液体菌种摇床培养的比较试验。结果表明，草菇液体菌种培养最适合的摇床转速是130 r/min；添加的维生素B₁的最佳浓度为15 mg/L；规格为250 mL的锥形瓶，培养基的最适装瓶量为80 mL；培养基静置36 h再进行摇床培养效果最好。
In order to screen suitable culture conditions of liquid strains for Volvariella volvacea, this experiment designed three formulas for comparative shaking table cultivation of V. volvacea liquid strains. The suitable medium formula was selected, namely 50 g of potato, 20 g of glucose, 1.5 g of peptone, 1 g of KH₂PO₄, 0.5 g of MgSO₄, 10 mg of Vitamin B₁, 1000 mL of water, and natural pH. On this basis, four factors were designed, including shaking table speed, vitamin B₁ addition concentration, bottling volume of culture medium, and settling time before shaking, to conduct comparative experiments on V. volvacea liquid strain shaking table cultivation. The results showed that the most suitable shaking table speed for the cultivation of V. volvacea liquid strains was 130 r/min; the optimal concentration of added vitamin B₁ was 15 mg/L; the optimal filling volume for a 250 mL conical flask and culture medium was 80 mL; the best effect was to let the culture medium stand for 36 hours before shaking it on a shaker.