|
|
松材线虫便携式分子检测系统的测试研究
|
Abstract:
随着我国木材及木质包装铺垫材料进境量的增大，口岸通关速度加快，对松材线虫检疫工作提出更高要求。本研究使用新型便携型松材线虫分子检测鉴定系统，对木屑样品和松材线虫样品进行测试。与常规检测方法相比，该系统灵敏度高，操作简单，适用于口岸一线松材线虫的快速检测。
With the increase in the import volume of wood and wooden packaging materials in China, the speed of port clearance has accelerated, and higher requirements have been put forward for the quarantine of pine wood nematodes. Using a new portable molecular detection and identification system for pine wood nematodes to test sawdust samples and pine wood nematode samples is done in this research. Compared with conventional detection methods, this system has high sensitivity, simple operation, and is suitable for rapid detection of pine wood nematodes on the first line of ports.
| [1] | Nickle, W.R., Golden, A.M., Mamiya, Y., et al. (1981) On the Taxonomy and Morphology of the Pine Wood Nematode, Bursaphelenchus xylophilus (Steiner & Buhrer 1934) Nickle 1970. Journal of Nematology, 13, 385-392. |
| [2] | Mamiya, Y. (1972) Pine Wood Nematode, Bursaphelenchus lignicolus Mamiya and Kiyohara, as a Causal Agent of Pine Wilting Disease. Review of Plant Protection Research, 5, 46-60. |
| [3] | Paramonov, A.A. (1967) A Critical Review of the Suborder Tylenchina (Filipjev, 1934) Nematoda: Secernentea. Trudy Gelmintologicheskoi Laboratorii Akademiya Nauk SSSR. |
| [4] | 叶为民, 冯志新. 松材线虫的形态学观察[J]. 动植物检疫, 1993(1): 14-16. |
| [5] | Mamiya, Y. (1988) History of Pine Wilt Disease in Japan. Journal of Nematolog, 20, 219-226. |
| [6] | Kishiy (1995) The Wood Nematode and the Japanese Pine Sawyer. Thomas Company Limited, Tokyo, 302. |
| [7] | 叶建仁. 松材线虫病在中国的流行现状、防治技术与对策分析[J]. 林业科学, 2019, 55(9): 1-10. |
| [8] | 李凤迪. 基于深度学习和无人机遥感的松材线虫病树监测系统研究[D]: [硕士学位论文]. 泰安: 山东农业大学, 2020. |
| [9] | 王曦茁, 曹业凡, 汪来发, 等. 松材线虫病发生及防控现状[J]. 环境昆虫学报, 2018, 40(2): 256-267. |
| [10] | 禄鑫, 冯陆春, 吴学卷, 等. 我国松材线虫病发生趋势及监测研究进展[J]. 贵州林业科技, 2023, 51(2): 105-110. |
| [11] | 于海英, 吴昊, 黄瑞芬, 等. 辽宁抚顺樟子松松材线虫分离与鉴定[J]. 中国森林病虫, 2020, 39(2): 6-10. |
| [12] | 武红敢, 牟晓伟, 杨清钰, 等. 无人机遥感技术在重庆市沙坪坝区松材线虫病监测中的应用[J]. 林业资源管理, 2019(2): 109-115. |
| [13] | 曾艳双. 松材线虫病的为害情况及防治措施浅析[J]. 南方农业, 2021, 15(5): 22-23. |
| [14] | 潘龙, 理永霞, 崔嵘, 等. 湖北日本落叶松中松材线虫的分离与鉴定[J]. 温带林业研究, 2020, 3(4): 52-56. |
| [15] | 于海英, 吴昊, 张旭东, 等. 落叶松自然条件下感染松材线虫初报[J]. 中国森林病虫, 2019, 38(4): 7-10. |
| [16] | 于海英, 吴昊. 辽宁发现松材线虫新寄主植物和新传播媒介昆虫[J]. 中国森林病虫, 2018, 37(5): 61. |
| [17] | 张志国. 国家林草局: 我国将开展松材线虫病防控5年攻坚行动[J]. 绿色中国, 2020(15): 70-71. |
| [18] | 孙铮, 张吉, 王荣, 等. 中国松材线虫病现状及检疫方法[J]. 现代农业科技, 2011(9): 177+187. |
| [19] | 张永宏, 李凯兵. 口岸快速检疫松材线虫技术初探[C]//中国昆虫学会, 中国植物病理学会. 外来有害生物检疫及防除技术学术研讨会论文汇编. 2005: 2. |
| [20] | 韩增强, 张文萍. 松材线虫鉴定技术初探[J]. 河南林业科技, 2019, 39(2): 32-54. |
| [21] | 曹福祥, 龙绛雪, 滕涛, 等. 3个株系松材线虫电镜扫描比较研究[J]. 中南林学院报, 2006(6): 103-106. |
| [22] | Stilwell, N., Whittington, R., Hick, P., Becker, J., Ariel, E., van Beurden, S., et al. (2018) Partial Validation of a Taqman Real-Time Quantitative PCR for the Detection of Ranaviruses. Diseases of Aquatic Organisms, 128, 105-116. https://doi.org/10.3354/dao03214 |
| [23] | 赵小金, 张盛静, 白昌明, 等. 牡蛎疱疹病毒魁蚶株交叉引物等温扩增检测方法的建立及初步应用[J]. 水产学报, 2015, 39(4): 580-588. |
| [24] | 张路平, 李纪标, 孔繁瑶. 松材线虫和拟松材线虫扫描电镜结构的比较研究[J]. 电子显微学报, 2000, 19(5): 679-684. |
| [25] | 杨宝君, 王秋丽, 邹卫东, 等. 关于拟松材线虫对松树致病性的研究[M]//中国松材线虫的流行与治理. 北京: 中国林业出版社, 1995: 47-49. |
| [26] | 廖月华, 温志海, 廖燕燕, 等. 休眠松材线虫幼虫快速检疫方法[P]. CN 200510018185. 2024-02-27. |
| [27] | Harmey, J.H. and Harmey, M.A. (1993) Detection and Identification of Bursaphelenchus Species with DNA Fingerprinting and Polymerase Chain Reaction. Nematology, 25, 406-415. |
| [28] | Iwahori, H., Kanzaki, N. and Futai, K. (2000) A Simple, Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism‐Aided Diagnosis Method for Pine Wilt Disease. Forest Pathology, 30, 157-164. https://doi.org/10.1046/j.1439-0329.2000.00201.x |
| [29] | 贺水山, 闻伟刚, 杨兰英, 等. 松材线虫PCR快速检测方法研究[J]. 植物检疫, 2002, 16(6): 321-324. |
| [30] | Takuya Aikawa, Natsumi Kanzaki, Taisei Kikuchi. 利用环介导恒温扩增法简易诊断松萎蔫病[J]. 南京林业大学学报: 自然科学版, 2011, 35(4): 148-149. |
| [31] | Cha, D., Kim, D., Choi, W., Park, S. and Han, H. (2020) Point-of-Care Diagnostic (POCD) Method for Detecting Bursaphelenchus xylophilus in Pinewood Using Recombinase Polymerase Amplification (RPA) with the Portable Optical Isothermal Device (POID). PLOS ONE, 15, e0227476. https://doi.org/10.1371/journal.pone.0227476 |
| [32] | 朱光前. 2017年中国木材进口及中非木材贸易简况[J]. 国际木业, 2018, 48(2): 14-21. |
| [33] | 李瑞法, 张学勤, 陈莹, 等. 我国进境原木主要疫情分析[J]. 中国森林病虫, 2015, 34(5): 8-11, 35. |
| [34] | 李海燕, 熊惠龙, 宋玉双, 等. 疫木保湿直接培养松材线虫的研究[J]. 中国森林病虫, 2008, 27(1): 38-40. |
