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Cav3通道阻滞剂可减轻膝骨性关节炎的软骨退变
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Abstract:
目的：本研究旨在观察T型钙离子通道(Cav3)阻滞剂对骨关节炎大鼠模型中软骨退变的治疗效果，并对其缓解退变的机制进行初步探讨。方法：对大鼠进行前交叉韧带横断(ACLT)诱导关节退变，造模后第3天开始予以Cav3阻滞剂TTA-Q6膝关节注射治疗，一周两次，每次100 μM，ACLT对照组以及假手术组予以生理盐水注射治疗，造模后30天和60天检查相应膝关节软骨病理学改变，使用免疫荧光染色测定软骨中二型胶原蛋白(Collagen II)和十型胶原蛋白(Collagen X)的表达。结果：ACLT大鼠软骨退变进行性加重，予以TTA-Q6治疗后，ACLT + TTA-Q6组软骨退变情况得到缓解，并且治疗后，软骨细胞十型胶原蛋白合成降低，二型胶原蛋白合成增高。结论：Cav3通道阻滞剂可以缓解软骨退变，这一作用可能是通过减轻软骨细胞肥大实现的。
Objective: The aim of this study was to observe the therapeutic effect of T-type calcium channel (Cav3) blocker on cartilage degeneration in rat model of osteoarthritis, and to explore the mechanism of alleviating the degeneration. Methods：Anterior cruciate ligament transection (ACLT) induced joint degeneration was performed on rats, Cav3 blocker TTA-Q6 was injected into knee joint twice a week, 100 μm each time, ACLT control group and sham operation group were injected with normal saline, and the corresponding pathological changes of knee joint cartilage were examined 30 and 60 days after modeling. The expression of Collagen II and Collagen X in cartilage was determined by immunofluorescence staining. Results: The cartilage degeneration of ACLT rats was progressively aggravated. After treatment with TTA-Q6, the cartilage degeneration of ACLT + TTA-Q6 group was alleviated, and after treatment, Type 10 collagen synthesis of chondrocytes was decreased and type 2 collagen synthesis was increased. Conclusion: Cav3 channel blockers can alleviate cartilage degeneration, which may be achieved by alleviating chondrocyte differentiation.
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