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微针贴片负载利多卡因在皮肤创面的镇痛研究
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Abstract:
目的：研发一种无痛、快速、经皮给药的局麻镇痛载药方式。方法：首先利用微成型技术制备出含有利多卡因的微针贴片(MNs-Lido)，对其进行物理、化学表征，利用大鼠足底切口疼痛模型检测MNs-Lido的镇痛效果，将大鼠随机分为3组：空白组(Blank组)、不载药物的MNs组(MNs组)和MNs-Lido组。大鼠术前先用Von-Frey测量机械缩足阈值(PWT)，使用异氟醚麻醉后，建立足底切口疼痛模型，术后1天记录各组大鼠的PWT。结果：Blank组和MNs组相比差异无统计学意义；与Blank组和MNs相比，MNs-Lido组的阈值在给药后15、30、45分钟时有显著改善，在60分钟时仍存在镇痛效果，因此证明利多卡因的麻醉效果可以维持60分钟。结论：MNs-Lido在皮肤创面镇痛中表现出有效的镇痛效果，其持续麻醉效果至少持续60分钟。本研究为皮肤创面镇痛提供了一种快速、无痛的局部麻醉方法。
Objective: To develop a painless, rapid, and transdermal local anesthesia and analgesia method. Methods: Firstly, microneedle patches loaded with lidocaine (MNs-Lido) were fabricated using microfabrication technology and characterized physically and chemically. The analgesic effect of MNs-Lido was evaluated using a rat plantar incision pain model. Rats were randomly divided into three groups: Blank group, MNs group without drug, and MNs-Lido group. The mechanical withdrawal threshold (PWT) was measured using the Von-Frey test before surgery. After isoflurane anesthesia, a plantar incision pain model was established, and PWT of each group was recorded 1 day after surgery. Results: There was no significant difference between the Blank group and MNs group. Compared with the Blank group and MNs group, PWT in the MNs-Lido group was significantly improved at 15, 30, and 45 minutes after administration, and the analgesic effect was still observed at 60 minutes, indicating that the anesthetic effect of lidocaine could last for at least 60 minutes. Conclusion: MNs-Lido exhibited effective analgesic effect on skin wounds, with a sustained anesthetic effect of at least 60 minutes. This study provides a rapid and painless local anesthesia method for analgesia of skin wounds.
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