Phytochemical Analysis and Antioxidant Activity of Aqueous and Hydroethanolic Extracts from Three Anticancerous Fabaceae of Northern Cameroon Pharmacopoeia
Background: Cancer continues to pose a significant threat to our society, representing one of the most pressing health concerns worldwide. This study aimed to evaluate the chemical composition and the antioxidant activity of aqueous and hydroethanolic extracts from Acacia nilotica (An), Bauhinia reticulate (Br), and Tamarindus indica (Ti) of Fabaceae family, traditionally used in Northern Cameroon for cancer treatment. Methods: The phytochemical screening of the three plants was conducted using conventional colorimetric methods, followed by the measurement of total phenol content, flavonoids, and tannins. The antiradical and antioxidant activities of both plant extracts were assessed through FRAP, ABTS, and DPPH methods. A principal components analysis was employed to correlate the quantities of the evaluated secondary metabolites with the activities. Results: Both types of extracts from the three plants contain alkaloids, saponins, flavonoids, phenolic compounds, tannins, glycosides, terpenoids, coumarins, anthocyanins, and anthraquinones. The aqueous extracts of Br and An are significantly richer (p < 0.05) in phenolic compounds and flavonoids than their respective hydroethanolic extracts, while the opposite is observed with Ti. The FRAP antioxidant potential was more pronounced in the aqueous extracts of Ti and Br than in their corresponding hydroethanolic extracts. However, An exhibited the highest potential with both types of extraction. The best DPPH scavenging activity was obtained with the aqueous extract of An, comparable to the reference. The same plant also demonstrated the highest activity in the ABTS test with both its extracts, more pronounced in the hydroethanolic extract, which was significantly better (p < 0.05) than the reference (gallic acid). The FRAP activity was highly correlated with the three classes of quantified secondary metabolites. Conclusion: The three Fabaceae plants from northern Cameroon, prepared in different solvents, can be utilized for their antiradical properties in cancer treatment.
References
[1]
World Health Organization (2024) Cancer. https://www.who.int/health-topics/cancer#tab=tab_1
[2]
Obistioiu, D., Cocan, I., Tîrziu, E., Herman, V., Negrea, M., Cucerzan, A., Neacsu, A.-G., Cozma, A.L., Nichita, I., Hulea, A., Radulov, I. and Alexa, E. (2021) Phytochemical Profile and Microbiological Activity of Some Plants Belonging to the Fabaceae Family. Antibiotics, 10, Article 662. https://doi.org/10.3390/antibiotics10060662
[3]
Ferlay, J., Colombet, M., Soerjomataram, I., Parkin, D.M., Piñeros, M., Znaor, A. and Bray, F. (2021) Cancer Statistics for the Year 2020: An Overview. International Journal of Cancer, 149, 778-789. https://doi.org/10.1002/ijc.33588
[4]
Siegel, R.L., Giaquinto, A.N. and Jemal, A. (2024) Cancer Statistics, 2024. CA: A Cancer Journal for Clinicians, 74, 12-49. https://doi.org/10.3322/caac.21820
[5]
World Health Organization (2023) Cancer. https://www.who.int/news-room/fact-sheets/detail/cancer
[6]
Bray, F., Parkin, D.M. and African Cancer Registry Network (2022) Cancer in Sub-Saharan Africa in 2020: A Review of Current Estimates of the National Burden, Data Gaps, and Future Needs. The LancetOncology, 23, 719-728. https://doi.org/10.1016/S1470-2045(22)00270-4
[7]
Sharma, R., Aashima, Nanda, M., Fronterre, C., Sewagudde, P., Ssentongo, A.E., Yenney, K., Arhin, N.D., Oh, J., Amponsah-Manu, F. and Ssentongo, P. (2022) Mapping Cancer in Africa: A Comprehensive and Comparable Characterization of 34 Cancer Types Using Estimates from GLOBOCAN 2020. Frontiers in Public Health, 10, Article 839835. https://doi.org/10.3389/fpubh.2022.839835
[8]
Nwaha, A. (2021) Cancer: 20745 nouveaux cas diagnostiqués au Cameroun en 2020. https://actucameroun.com/2021/10/21/cancer-20745-nouveaux-cas-diagnostiques-au-cameroun-en-2020/
[9]
World Health Organization (2024) Cancer. https://www.who.int/news-room/fact-sheets/detail/cancer
[10]
Shuel, S.L. (2022) Therapies ciblées contre le cancer. Canadian Family Physician, 68, e199-e203. https://doi.org/10.46747/cfp.6807e199
[11]
Thiombiano, M.H., Bangou, M.J., Ouoba, B., Sawadogo, M., Lema, A., Nacoulma, A.P., Ouoba, H.Y. and Ouedraogo, G.A. (2022) Plants and Medicinal Practices in Burkina Faso: The Case of Breast Cancer. Journal of Diseases and Medicinal Plants, 8, 46-54.
[12]
Usman, M., Khan, W.R., Yousaf, N., Akram, S., Murtaza, G., Kudus, K.A., Ditta, A., Rosli, Z., Rajpar, M.N. and Nazre, M. (2022) Exploring the Phytochemicals and Anti-Cancer Potential of the Members of Fabaceae Family: A Comprehensive Review. Molecules, 27, Article 3863. https://doi.org/10.3390/molecules27123863
[13]
Sadiq, M.B., Tharaphan, P., Chotivanich, K., Tarning, J. and Anal, A.K. (2017) In vitro Antioxidant and Antimalarial Activities of Leaves, Pods and Bark Extracts of Acacia nilotica (L.) Del. BMC Complementary and Alternative Medicine, 17, Article No. 372. https://doi.org/10.1186/s12906-017-1878-x
[14]
Auwal, M.S., Saka, S., Mairiga, I.A., Sanda, K.A., Shuaibu, A. and Ibrahim, A. (2014) Preliminary Phytochemical and Elemental Analysis of Aqueous and Fractionated Pod Extracts of Acacia nilotica (Thorn Mimosa). Veterinary Research Forum: An International Quarterly Journal, 5, 95-100.
[15]
Kamal, Y., Khan, T., Haq, I., Zahra, S.S., Asim, M.H., Shahzadi, I., Mannan, A. and Fatima, N. (2022) Phytochemical and Biological Attributes of Bauhinia variegata L. (Caesalpiniaceae). Brazilian Journal of Biology=Revista Brasleira de Biologia, 82, e257990. https://doi.org/10.1590/1519-6984.257990
[16]
Sookying, S., Duangjai, A., Saokaew, S. and Phisalprapa, P. (2022) Botanical Aspects, Phytochemicals, and Toxicity of Tamarindus indica Leaf and a Systematic Review of Antioxidant Capacities of T. indica Leaf Extracts. Frontiers in Nutrition, 9, Article 977015. https://doi.org/10.3389/fnut.2022.977015
[17]
Zali, M.A., Fonkoua, M., Dakole, C., Tazon, A.W., Goda, D., Akamba, B.D.A. and Nanhah, J.V. (2023) Comparative Study of the Effect of Two Extraction Solvents on Polyphenols Content and Antioxydant Activity of Alstonia boonei Bark. Asian Journal of Chemical Sciences, 13, 7-17. https://doi.org/10.9734/ajocs/2023/v13i4245
[18]
Egbuna, C., Ifemeje, J.C., Maduako, M.C., Tijjani, H., Udedi, S.C., Nwaka, A.C. and Ifemeje, M.O. (2018) Phytochemical Test Methods: Qualitative, Quantitative and Proximate Analysis. In: Egbuna, C., Ifemeje, J.C., Udedi, S.C. and Kumar, S., Eds., Phytochemistry, Apple Academic Press, New York, 46 p. https://doi.org/10.1201/9780429426223
[19]
Vinson, J.A., Su, X., Zubik, L. and Bose, P. (2001) Phenol Antioxidant Quantity and Quality in Foods: Fruits. Journal of Agricultural and Food Chemistry, 49, 5315-5321. https://doi.org/10.1021/jf0009293
[20]
Zhishen, J., Mengcheng, T. and Jianming, W. (1999) The Determination of Flavonoid Contents in Mulberry and Their Scavenging Effects on Superoxide Radicals. Food Chemistry, 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
[21]
Bainbridge, Z., Tomlins, K., Willings, K. and Westby, A. (1996) Methods for Assessing Quality Characteristics of Non-Grain Starch Staple. Part 4 Advanced Methods. National Resources Institute, University of Greenwich, Chatham, 43-79.
[22]
Benzie, I.F. and Strain, J.J. (1996) The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239, 70-76. https://doi.org/10.1006/abio.1996.0292
[23]
Blois, M.S. (1958) Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0 https://www.scirp.org/reference/ReferencesPapers?ReferenceID=1148740
[24]
Arnon, S.S., Schechter, R., Inglesby, T.V., Henderson, D.A., Bartlett, J.G., Ascher, M.S., Eitzen, E., Fine, A.D., Hauer, J., Layton, M., Lillibridge, S., Osterholm, M.T., O’Toole, T., Parker, G., Perl, T.M., Russell, P.K., Swerdlow, D.L., Tonat, K. and Working Group on Civilian Biodefense (2001) Botulinum Toxin as a Biological Weapon: Medical and Public Health Management. JAMA, 285, 1059-1070. https://doi.org/10.1001/jama.285.8.1059
[25]
Kopustinskiene, D.M., Jakstas, V., Savickas, A. and Bernatoniene, J. (2020) Flavonoids as Anticancer Agents. Nutrients, 12, Article 457. https://doi.org/10.3390/nu12020457
[26]
Liu, W., Cui, X., Zhong, Y., Ma, R., Liu, B. and Xia, Y. (2023) Phenolic Metabolites as Therapeutic in Inflammation and Neoplasms: Molecular Pathways Explaining Their Efficacy. Pharmacological Research, 193, Article 106812. https://doi.org/10.1016/j.phrs.2023.106812
[27]
Kleszcz, R., Majchrzak-Celinska, A. and Baer-Dubowska, W. (2023) Tannins in Cancer Prevention and Therapy. British Journal of Pharmacology. https://doi.org/10.1111/bph.16224
[28]
Teoh, E.S. (2015) Secondary Metabolites of Plants. In: Medicinal Orchids of Asia, Springer, Cham, 59-73. https://doi.org/10.1007/978-3-319-24274-3_5
[29]
Mutha, R.E., Tatiya, A.U. and Surana, S.J. (2021) Flavonoids as Natural Phenolic Compounds and Their Role in Therapeutics: An Overview. Future JournalofPharmaceuticalSciences, 7, 25. https://doi.org/10.1186/s43094-020-00161-8
[30]
Brunetti, C., Di Ferdinando, M., Fini, A., Pollastri, S. and Tattini, M. (2013) Flavonoids as Antioxidants and Developmental Regulators: Relative Significance in Plants and Humans. International Journal of Molecular Sciences, 14, 3540-3555. https://doi.org/10.3390/ijms14023540
[31]
Barajas-Ramirez, J.A., Cabrera-Ramirez, A.H. and Aguilar-Raymundo, V.G. (2023) Antioxidant Activity, Total Phenolic, Tannin, and Flavonoid Content of Five Plants Used in Traditional Medicine in Penjamo, Guanajuato. Chemistry & Biodiversity, 20, e202200834. https://doi.org/10.1002/cbdv.202200834
[32]
Kováč, J., Slobodníková, L., Trajčíková, E., Rendeková, K., Mučaji, P., Sychrová, A. and Bittner Fialová, S. (2023) Therapeutic Potential of Flavonoids and Tannins in Management of Oral Infectious Diseases—A Review. Molecules, 28, Article 158. https://doi.org/10.3390/molecules28010158
[33]
Khan, M.I., Karima, G., Khan, M.Z., Shin, J.H. and Kim, J.D. (2022) Therapeutic Effects of Saponins for the Prevention and Treatment of Cancer by Ameliorating Inflammation and Angiogenesis and Inducing Antioxidant and Apoptotic Effects in Human Cells. International Journal of Molecular Sciences, 23, Article 10665. https://doi.org/10.3390/ijms
[34]
Anantharaju, P.G., Gowda, P.C., Vimalambike, M.G. and Madhunapantula, S.V. (2016) An Overview on the Role of Dietary Phenolics for the Treatment of Cancers. Nutrition Journal, 15, Article No. 99. https://doi.org/10.1186/s12937-016-0217-2
[35]
Mojzer, E.B., Hrnčič, M.K., Škerget, M., Knez, Ž. and Bren, U. (2016) Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects. Molecules, 21, Article 901. https://doi.org/10.3390/molecules21070901
[36]
Seca, A.M.L. and Pinto, D.C.G.A. (2018) Plant Secondary Metabolites as Anticancer Agents: Successes in Clinical Trials and Therapeutic Application. International Journal of Molecular Sciences, 19, Article 263. https://doi.org/10.3390/ijms19010263
