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基于微流控芯片的微球计数方法研究
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Abstract:
微球荧光微球是一种圆形球状颗粒，在医学研究中，常被用于模拟病毒传播痕迹。传统的流式细胞仪测量微球个数时不仅所耗样品多，且成本高。为此，本文基于微流控方法，结合光学检测技术，开发了一种微球快数精确计数的方法，研究了纯水、Tris-硼酸、羟乙基纤维素(HEC)分别作为背景液时微球在微通道内的运动状况。结果表明，在HEC作为缓冲时，即使一个微球通过微流控芯片的检测窗口时，也可以获取其检测信号，微球检测通量约为400个/分钟。本文所报道的微流控芯片方法有望实验提供低成本、简便快捷的微球计数工作的开发提供技术指导。
Microsphere fluorescent microspheres are circular spherical particles commonly used in medical research to simulate traces of virus transmission. Traditional flow cytometry not only consumes a lot of samples but also has high cost when measuring the number of microspheres. Therefore, based on microfluidic methods and optical detection technology, this article developed a method for accurately counting the fast number of microspheres, and studied the motion of microspheres in micro channels when pure water, Tris boric acid, and hydroxyethyl cellulose (HEC) were used as background liquids. The results showed that when HEC was used as a buffer, even when a microsphere passed through the detection window of the microfluidic chip, its detection signal could still be obtained, and the microsphere detection flux was about 400 per minute. The micro fluidic chip method reported in this article is expected to provide technical guidance for the development of low-cost, convenient, and fast microsphere counting experiments.
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