扶正散结方对肺腺癌Bax、Bcl-2表达及肿瘤生长的影响
Effects of Fu Zheng San Jie Fang on Bax and Bcl-2 Expression and Tumour Growth in Lung Adenocarcinoma

目的：观察扶正散结方对肺腺癌Bax、Bcl-2表达及肿瘤生长的作用效果。方法：建立肺腺癌H1299细胞裸鼠移植瘤模型，将20只荷瘤鼠随机分成模型对照组(后简称对照组)和扶正散结方干预组(2.275 g/mL) (后简称扶正散结方组)、扶正散结方联合阳性药物替吉奥干预组(后简称联合组)及阳性药物替吉奥对照组(后简称阳照组)，每1 d灌胃给药1次，于每次给药前测定各组荷瘤鼠体质量，药物干预21 d后处死小鼠，剥离瘤体并称量瘤体质量，采用免疫组织化学染色检测不同处理组细胞Bax、Bcl-2表达水平。同时体外培养H1299细胞，应用细胞计数试剂盒(CCK-8)检测不同浓度扶正散结方对细胞增殖的影响。结果：与对照组[(22.8 1.0) g]比较，联合组[(19.10 ± 1.18) g]、阳照组[(20.10 ± 0.17) g]干预21 d后小鼠体质量增长均有明显下降(P < 0.05)。与对照组瘤体质量(2.02 ± 0.38) g相比，扶正散结方组、联合组、阳照组瘤体质量分别为(0.77 ± 0.25) g、(0.64 ± 0.43) g、(0.74 ± 0.27) g，与对照组相比均明显减少，差异有统计学意义(P < 0.05)。免疫组化结果显示，扶正散结方组、联合组和阳照组的Bax蛋白表达水平均高于对照组，Bcl-2表达水平低于对照组，差异均有统计学意义(P < 0.05)。各浓度扶正散结方均可对体外培养的H1299细胞的增殖产生抑制效果，且此抑制效果随药物浓度增加与干预时间延长而提高。结论：扶正散结方对肺腺癌生长具有抑制作用，其可通过上调促凋亡蛋白，下调抗凋亡蛋白的表达达成上述目标。同时体外实验也证明，扶正散结方对肺腺癌H1299细胞的增殖具有抑制作用。即扶正散结方可通过促进凋亡及抑制增殖两方面实现抑制肿瘤生长。
Objective: To observe the effects of Fu Zheng San Jie Fang on the expression of Bax and Bcl-2 and tumour growth in lung adenocarcinoma. Methods: A lung adenocarcinoma transplantation model was established in nude mice with H1299 cells, and 20 tumor-bearing mice were randomly divided into a model control group (later referred to as the control group), an intervention group of Fu Zheng San Jie Fang (2.275 g/mL) (later referred to as the group of Fu Zheng San Jie Fang), an intervention group of Fu Zheng San Jie Fang combined with a positive drug, Tegio (later referred to as the combined group), and a control group of a positive drug, Tegio (later referred to as the positive control group), and the drug was administered by gavage once every 1 d. The body mass of rats in each group was measured before each administration of the drug, and the mice were executed after 21 d of drug intervention, the tumours were stripped and weighed, and the expression levels of Bax and Bcl-2 in the cells of different treatment groups were detected by immunohistochemical staining. At the same time, H1299 cells were cultured in vitro, and a cell counting kit (CCK-8) was applied to detect the effects of different concentrations of Fu Zheng San Jie Fang on cell proliferation. Results: Compared with the control group [(22.8 1.0) g], there was a significant decrease in the growth of body mass of mice in the combined group [(19.10 ± 1.18) g] and the Yang-photographed group [(20.10 ± 0.17) g] after 21 d of the intervention (P < 0.05). Compared with the tumour mass of the control group (2.02 ± 0.38) g, the tumour mass of the Fu Zheng San Jie Fang group, the combined group and the Yang Zhi group were (0.77 ± 0.25) g, (0.64 ± 0.43) g and (0.74 ± 0.27) g,