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基于网络药理学研究丹参饮与补心气口服液治疗冠心病的分子机制
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Abstract:
目的：采用网络药理学方法解析中药治疗冠心病的药理机制。方法：使用中药系统药理学分析平台(TCMSP)，检索丹参、檀香、砂仁、黄芪、人参、石菖蒲、薤白7味中药的有效成分和作用靶点。使用DDrugBank、TTD、In-Cardiome三个数据库中收集冠心病相关靶点。利用Cytoscape软件构建丹参、补心气口服液–冠心病靶点网络和靶点–通路网络。通过DAVID数据库进行生物功能和信号通路富集分析，预测丹参饮治疗冠心病的作用机制。结果：通过数据库筛选出得到丹参饮和补心气口服液作用的冠心病靶点，分别为68、97个，信号通路各10条，并利用分子对接技术筛选出前九个补心气口服液和丹参饮的成分与冠心病靶点对接的结合能排名。结论：丹参饮与补心气口服液可能通过多靶点、多信号通路协同治疗冠心病。
Objective: This paper aims to analyze the pharmacological mechanism of traditional Chinese medicine (TCM) in the treatment of coronary heart disease (CHD). Method: By using the TCM Systematic pharmacological analysis platform (TCMSP), this paper searches the effective components and targets of salvia miltiorrhiza, sandalwood, amomum, Astragalus, ginseng, Calamus, Allium Macrostemon 7. Three databases, DDrugBank, TTD and In-Cardiome, were used to collect CHD related targets. The target network and target pathway network of Salvia miltiorrhiza, and Buxinqi oral liquid were constructed by Cytoscape software. The biological function and signal pathway enrichment analysis were conducted through DAVID database to predict the mechanism of action of Danshen Decoction in the treatment of coronary heart disease. Results: 68 and 97 CHD targets of Danshen Decoction and Buxinqi oral liquid were selected through the database, and 10 signal pathways were obtained, and the binding energy ranking of the first nine Buxinqi oral liquid and Danshen decoction with CHD target was selected by molecular docking technology. Conclusion: Danshen Decoction and Buxinqi oral liquid may treat coronary heart disease through multi-target and multi-signal pathway synergism.
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