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丁香提取物通过调控细胞周期促进卵巢癌细胞增殖
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Abstract:
研究酒精丁香提取物(Alcohol Extract of Clove, AEC)对人源性卵巢癌细胞A2780增殖抑制和周期的影响及其潜在的作用机制。方法：CCK8法检测A2780卵巢癌细胞经AEC作用后的增殖情况；克隆形成检测A2780细胞群体依赖性情况，软琼脂克隆法检测AEC作用后的成团能力；Transwell试验检测与划痕实验细胞迁移情况；流式细胞术检测细胞周期情况。结果：CCK8实验结果提示AEC具有抑制卵巢癌细胞的增殖活性，并呈浓度依赖性；平板克隆与软琼脂克隆结果说明了AEC抑制卵巢癌细胞的生长；划痕实验和Transwell实验结果显示AEC作用卵巢癌细胞后抑制了迁移能力卵巢癌细胞经AEC处理后显微镜观察细胞形态发生变化并促进细胞的凋亡。流式细胞周期结果显示AEC可以引起G1期和S期增多。结论：AEC对卵巢癌细胞系有增殖抑制的作用，抑制细胞迁移并促进卵巢癌的凋亡并改变卵巢癌细胞的形态，将周期抑制在G1期和S期。
Exploring the potential mechanism of action of alcoholic extract of clove (AEC) in inhibiting proliferation and cycling of human ovarian cancer A2780 cells, this paper seeks to investigate its effect. The CCK8 assay indicated that AEC had a concentration-dependent effect on ovarian cancer cell proliferation; plate cloning and soft agar cloning revealed that AEC inhibited ovarian cancer cell growth; and the scratch assay and Transwell assay demonstrated that AEC had a hindrance on ovarian cancer cell migration following AEC treatment. The results of cell cycle analysis showed that AEC induced growth in G1 and S phases. To sum up, AEC hinders the growth of ovarian cancer cell lines, obstructs cell movement, encourages apoptosis, modifies the shape of ovarian cancer cells, and impedes the G1 and S phases of the cell cycle.
| [1] | Sung, H., Ferlay, J., Siegel, R.L., et al. (2021) Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians, 71, 209-249. https://doi.org/10.3322/caac.21660 |
| [2] | Katiyi, A., Zorea, J., Halstuch, A., et al. (2020) Surface Roughness-Induced Absorption Acts as an Ovarian Cancer Cells Growth Sensor-Monitor. Biosensors & Bioelectronics, 161, Article 112240. https://doi.org/10.1016/j.bios.2020.112240 |
| [3] | Xiao, Y., Yu, Y., Jiang, P., et al. (2020) The PI3K/MTOR Dual Inhibitor GSK458 Potently Impedes Ovarian Cancer Tumorigenesis and Metastasis. Cellular Oncology, 43, 669-680. https://doi.org/10.1007/s13402-020-00514-8 |
| [4] | Wang, Z., Chen, W., Zuo, L., et al. (2022) The Fibrillin-1/VEGFR2/STAT2 Signaling Axis Promotes Chemoresistance via Modulating Glycolysis and Angiogenesis in Ovarian Cancer Organoids and Cells. Cancer Communications, 42, 245-265. https://doi.org/10.1002/cac2.12274 |
| [5] | Leng, J., Lei, L., Lei, S.F., et al. (2020) Use of Traditional Chinese Herbal Medicine Concurrently with Conventional Cancer Treatment among Chinese Cancer Patients. Journal of Immigrant and Minority Health, 22, 1240-1247. https://doi.org/10.1007/s10903-020-01017-2 |
| [6] | Haro-González, J.N., Castillo-Herrera, G.A., Martínez-Velázquez, M., et al. (2021) Clove Essential Oil (Syzygium aromaticum L. Myrtaceae): Extraction, Chemical Composition, Food Applications, and Essential Bioactivity for Human Health. Molecules, 26, Article 6387. https://doi.org/10.3390/molecules26216387 |
| [7] | Hui, Q., Ammeter, E., Liu, S., et al. (2020) Eugenol Attenuates Inflammatory Response and Enhances Barrier Function during Lipopolysaccharide-Induced Inflammation in the Porcine Intestinal Epithelial Cells. Journal of Animal Science, 98, Article skaa245. https://doi.org/10.1093/jas/skaa245 |
| [8] | De Oliveira, A.S., Gazolla, P.A.R., Oliveira, A., et al. (2019) Discovery of Novel West Nile Virus Protease Inhibitor Based on Isobenzonafuranone and Triazolic Derivatives of Eugenol and Indan-1,3-Dione Scaffolds. PLOS ONE, 14, e0223017. https://doi.org/10.1371/journal.pone.0223017 |
| [9] | Hu, Q., Zhou, M. and Wei, S. (2018) Progress on the Antimicrobial Activity Research of Clove Oil and Eugenol in the Food Antisepsis Field. Journal of Food Science, 83, 1476-1483. https://doi.org/10.1111/1750-3841.14180 |
| [10] | Barboza, J.N., Da Silva Maia Bezerra Filho, C., Silva, R.O., et al. (2018) An Overview on the Anti-Inflammatory Potential and Antioxidant Profile of Eugenol. Oxidative Medicine and Cellular Longevity, 2018, Article ID: 3957262. https://doi.org/10.1155/2018/3957262 |
| [11] | Taher, Y.A., Samud, A.M., El-Taher, F.E., et al. (2015) Experimental Evaluation of Anti-Inflammatory, Antinociceptive and Antipyretic Activities of Clove Oil in Mice. Libyan Journal of Medicine, 10, Article 28685. https://doi.org/10.3402/ljm.v10.28685 |
| [12] | Liu, H., Schmitz, J.C., Wei, J., et al. (2014) Clove Extract Inhibits Tumor Growth and Promotes Cell Cycle Arrest and Apoptosis. Oncology Research, 21, 247-259. https://doi.org/10.3727/096504014X13946388748910 |
| [13] | Chua, L.K., Lim, C.L., Ling, A.P.K., et al. (2019) Anticancer Potential of Syzygium Species: A Review. Plant Foods for Human Nutrition, 74, 18-27. https://doi.org/10.1007/s11130-018-0704-z |
| [14] | Vázquez-Fresno, R., Rosana, A.R.R., Sajed, T., et al. (2019) Herbs and Spices-Biomarkers of Intake Based on Human Intervention Studies—A Systematic Review. Genes & Nutrition, 14, Article No. 18. https://doi.org/10.1186/s12263-019-0636-8 |
| [15] | 黄宏, 陶方方. 沈敏鹤复方附苓汤治疗卵巢癌经验研究[J]. 中国医药导报, 2023, 20(6): 132-135. |
| [16] | Shin, Y. and Lee, Y. (2013) Cytotoxic Activity from Curcuma zedoaria through Mitochondrial Activation on Ovarian Cancer Cells. Toxicological Research, 29, 257-261. https://doi.org/10.5487/TR.2013.29.4.257 |
| [17] | 王兴玲, 于明新. 苦参碱联合胸腺五肽对卵巢癌腹腔热化疗大鼠免疫功能的影响[J]. 实用药物与临床, 2017, 20(1): 4-8. |
| [18] | 汤欣, 韩凤娟, 李威, 等. 莪术醇对人卵巢癌SKOV3细胞株JAK2/STAT3信号通路影响的研究[J]. 中国妇产科临床杂志, 2013, 14(1): 43-46. |
| [19] | 李悦, 张艳, 郭滢, 等. 卵巢癌的中医药研究应用进展[J]. 辽宁中医杂志, 2016, 43(1): 194-196. |
| [20] | Barani, M., Bilal, M., Sabir, F., et al. (2021) Nanotechnology in Ovarian Cancer: Diagnosis and Treatment. Life Sciences, 266, Article 118914. https://doi.org/10.1016/j.lfs.2020.118914 |
| [21] | Matthews, H.K., Bertoli, C. and De Bruin, R.A.M. (2022) Cell Cycle Control in Cancer. Nature Reviews Molecular Cell Biology, 23, 74-88. https://doi.org/10.1038/s41580-021-00404-3 |
| [22] | Fagundes, R. and Teixeira, L.K. (2021) Cyclin E/CDK2: DNA Replication, Replication Stress and Genomic Instability. Frontiers in Cell and Developmental Biology, 9, Article 774845. https://doi.org/10.3389/fcell.2021.774845 |
| [23] | Carneiro, B.A. and El-Deiry, W.S. (2020) Targeting Apoptosis in Cancer Therapy. Nature Reviews Clinical Oncology, 17, 395-417. https://doi.org/10.1038/s41571-020-0341-y |
