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Impact of Dietary Lactobacillus plantarum Postbiotics on the Performance of Layer Hens under Heat Stress Conditions

DOI: 10.4236/ojvm.2024.143004, PP. 39-55

Keywords: Lactobacillus plantarum, Layers, Heat Stress, Postbiotic, Probiotics, Performance

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This experiment was conducted to determine the performance of heat-stressed layers fed a diet containing the probiotic Lactobacillus plantarum RS5 or its products of fermentation (postbiotics). Twenty-week-old Isa White layers, were subdivided into six treatments of 32 individually caged birds. Half of the birds were reared under regular temperature conditions, while the other half was subjected to cyclic daily heat stress. Layers were offered one of three diets: 1) Control; 2) Control + Lactobacillus plantarum RS5 probiotic; 3) Control + Lactobacillus plantarum RS5 postbiotics. Birds were tested for performance and visceral organ development for 5 months. Heat stress negatively affected the birds’ feed intake, egg weight, shell weight percentage, Haugh unit, shell thickness, yolk color, body weight and spleen weight percentage. Postbiotics significantly increased egg production (p < 0.05) in comparison to the control and the probiotic fed group (94.8% vs 92.6% vs 93.1%, respectively). Birds under probiotic or postbiotic diet showed a significantly higher (p < 0.05) feed intake and egg weight, although the probiotic had a more pronounced and gradual effect. Specific gravity, yolk weight percentage and shell thickness didn’t show differences among dietary groups. The Haugh Unit was significantly higher (p < 0.05) in probiotic group which also showed a significantly lower yolk color index (p < 0.05). The different feed treatments did not impact the bird’s viscera weight percentage, except for the ileum that was significantly lower (p < 0.05) under postbiotic supplementation. Both probiotics and postbiotics could be used as a potential growth promoters and might alleviate heat stress impact in poultry industry.


[1]  Ajakaiye, J.J., Cuesta-Mazorra, M. and Garcia-Diaz, J.R. (2011) Vitamins C and E Can Alleviate Adverse Effects of Heat Stress on Live Weight and Some Egg Quality Profiles of Layer Hens. Pakistan Veterinary Journal, 31, 45-49.
[2]  Quinteiro-Filho, W.M., Ribeiro, A., Ferraz-De-Paula, V., Pinheiro, M., Sakai, M., Sa, L.R.M., Ferreira, A.J.P. and Palermo-Neto, J. (2010) Heat Stress Impairs Performance Parameters, Induces Intestinal Injury, and Decreases Macrophage Activity in Broiler Chickens. Poultry Science, 89, 1905-1914.
[3]  Loh, T.C., Choe, D.W., Foo, H.L., Sazili, A.Q. and Bejo, M.H. (2014) Effects of Feeding Different Postbiotic Metabolite Combinations Produced by Lactobacillus plantarum Strains on Egg Quality and Production Performance, Faecal Parameters and Plasma Cholesterol in Laying Hens. BMC Veterinary Research, 10, Article No. 149.
[4]  Shazali, N., Foo, H.L., Loh, T.C., Choe, D.W. and Rahim, R.A. (2014) Prevalence of Antibiotic Resistance in Lactic Acid Bacteria Isolated from the Faeces of Broiler Chicken in Malaysia. Gut Pathogens, 6, Article No. 1.
[5]  Van Boeckel, T.P., Brower, C., Gilbert, M., Grenfell, B.T., Levin, S.A., Robinson, T.P., Teuillant, A. and Laxminarayan, R. (2015) Global Trends in Antimicrobial Use in Food Animals. Proceedings of the National Academy of Sciences of the United States of America, 112, 5649-5654.
[6]  Lokapirnasari, W.P., Teguh, B.P., Anam, A.A., Soeharsono, S., Sri, H., Nenny, H., Rifqy, N., Khoirul, H., Hana, C.P.W., Nabil, F.N.R. and Andreas, B.Y. (2019) Potency of Probiotics Bifidobacterium spp. and Lactobacillus casei to Improve Growth Performance and Business Analysis in Organic Laying Hens. Veterinary World, 12, 860-867.
[7]  Prabhurajeshwara, C. and Kelmani, C. (2019) Evaluation of Antimicrobial Properties and Their Substances against Pathogenic Bacteria In-Vitro by Probiotic Lactobacilli Strains Isolated from Commercial Yoghurt. Clinical Nutrition Experimental, 23, 97-115.
[8]  Shokryazdan, P., Faseleh Jahromi, M., Liang, J.B., Ramasamy, K., Sieo, C.C. and Ho Y.W. (2017) Effects of a Lactobacillus salivarius Mixture on Performance, Intestinal Health and Serum Lipids of Broiler Chickens. PLOS ONE, 12, e0175959.
[9]  Julio, P.D., Francisco, J.R.O., Mercedes, G.C. and Angel, G. (2019) Mechanisms of Action of Probiotics.
[10]  Thanh, N.T., Loh, T.C., Foo, H.L., Hair-Bejo, M. and Azhar, B.K. (2009) Effects of Feeding Metabolite Combinations Produced by Lactobacillus plantarum on Growth Performance, Faecal Microbial Population, Small Intestine Villus Height and Faecal Volatile Fatty Acids in Broilers. British Poultry Science, 50, 298-306.
[11]  Humam, A.Z., Loh, T.C., Foo, H.L., Samsudin, A.A., Noordin, M.M., Zulkifli, I. and Izuddin, W.I. (2019) Effects of Feeding Different Postbiotics Produced by Lactobacillus plantarum on Growth Performance, Carcass Yield, Intestinal Morphology, Gut Microbiota Composition, Immune Status, and Growth Gene Expression in Broilers under Heat Stress. Animals, 9, Article No. 644.
[12]  Fajardo, P., Lorenzo, P., Jesús, M., Isabel, R., Clara, F. and Nelson, P.G. (2012) Effects of Feeding of Two Potentially Probiotic Preparations from Lactic Acid Bacteria on the Performance and Faecal Microflora of Broiler Chickens. World Journal, 2012, Article ID: 562635.
[13]  Izuddin, W.I., Loh, T.C., Foo, H.L., Samsudin, A.A. and Humam, A.M. (2019) Postbiotic Lactobacillus plantarum RG14 Improves Ruminal Epithelium Growth, Immune Status and Upregulates the Intestinal Barrier Function in Post-Weaning Lambs. Scientific Reports, 9, Article No. 9938.
[14]  Ji, K., Jang, N.Y. and Kim, Y.T. (2015) Isolation of Lactic Acid Bacteria Showing Antioxidative and Probiotic Activities from Kimchi and Infant Feces. Journal of Microbiology and Biotechnology, 25, 1568-1577.
[15]  Kareem, K.Y., Ling, F.H., Chwen, L.H., Foong, O.M. and Asmara, S.A. (2014) Inhibitory Activity of Postbiotic Produced by Strains of Lactobacillus plantarum Using Reconstituted Media Supplemented with Inulin. Gut Pathogens, 6, Article No. 23.
[16]  Allahverdi, A., Feizi, A., Takhtfooladi, H.A. and Nikpiran, H. (2013) Effects of Heat Stress on Acid-Base Imbalance, Plasma Calcium Concentration, Egg Production and Egg Quality in Commercial Layers. Global Veterinaria, 10, 203-207.
[17]  Deng, W., Dong, X.F., Tong, J.M. and Zhang, Q. (2012) The Probiotic Bacillus licheniformis Ameliorates Heat Stress-Induced Impairment of Egg Production, Gut Morphology, and Intestinal Mucosal Immunity in Laying Hens. Poultry Science, 91, 575-582.
[18]  Lara, L.J. and Rostagno, M.H. (2013) Impact of Heat Stress on Poultry Production. Animals, 3, 356-369.
[19]  Soleimani, F., Zulkifli, I., Omar, A.R. and Raha, A.R. (2011) Physiological Responses of 3 Chicken Breeds to Acute Heat Stress. Poultry Science, 90, 1435-1440.
[20]  Menconi, A., Kallapura, G., Latorre, J.D., Morgan, M.J., Pumford, N.R., Hargis, B.M. and Tellez, G. (2014) Identification and Characterization of Lactic Acid Bacteria in a Commercial Probiotic Culture. Bioscience of Microbiota, Food and Health, 33, 25-30.
[21]  Peralta-Sánchez, J.M., Martín-Platero, A.M., Ariza-Romero, J.J., Rabelo-Ruiz, M., Zurita-González, M.J., Baños, A., Rodriguez-Ruano, S.M., Maqueda, M., Valdivia, E. and Martínez-Bueno, M. (2019) Egg Production in Poultry Farming Is Improved by Probiotic Bacteria. Frontiers in Microbiology, 10, Article No. 1042.
[22]  De Rensis, F., Scaramuzzi, R.J. (2003) Heat Stress and Seasonal Effects on Reproduction in the Dairy Cow—A Review. Theriogenology, 60, 1139-1151.
[23]  Song, J., Xiao, K., Ke, Y.L., Jiao, L.F., Hu, C.H., Diao, Q.Y., Shi, B. and Zou, X.T. (2014) Effect of a Probiotic Mixture on Intestinal Microflora, Morphology, and Barrier Integrity of Broilers Subjected to Heat Stress. Poultry Science, 93, 581-588.
[24]  Zhang, L., Zhang, L., Zhan, X.A., Zeng, X., Zhou, L., Cao, G., Chen, A. and Yang, C. (2016) Effects of Dietary Supplementation of Probiotic, Clostridium butyricum, on Growth Performance, Immune Response, Intestinal Barrier Function, and Digestive Enzyme Activity in Broiler Chickens Challenged with Escherichia coli K88. Journal of Animal Science and Biotechnology, 7, Article No. 3.
[25]  Neupane, D., Nepali, D., Devkota, N., Sharma, M. and Kadaria, I. (2019) Effect of Probiotics on Production and Egg Quality of Dual Purpose Chicken at Kathmundu in Nepal. Bangladesh Journal of Animal Science, 48, 29-35.
[26]  Star, L., Juul-Madsen, H.R., Decuypere, E., Nieuwland, M.G., De Vries Reilingh, G., Van Den Brand, H., Kemp, B. and Parmentier, H.K. (2009) Effect of Early Life Thermal Conditioning and Immune Challenge on Thermotolerance and Humoral Immune Competence in Adult Laying Hens. Poultry Science, 88, 2253-2261.
[27]  Mack, L.A., Felver-Gant, J.N., Dennis, R.L. and Cheng, H.W. (2013) Genetic Variations Alter Production and Behavioral Responses Following Heat Stress in 2 Strains of Laying Hens. Poultry Science, 92, 285-294.
[28]  Wiernusz, C. (1998) Nutritional Therapies to Optimize Poultry Production during High Humidity and Ambient Temperature Exposure. Technical News, Quarterly Publication of Cobb-Vantress, Incorporated, Arkansas, 1-6.
[29]  Zaviezo, R. (1999) Nutritional Management of Birds Affected by Heat. Revue de l' Industrie Avicole, 46, 42-46.
[30]  Bonnet, S., Geraert, P.A., Lessire, M., Carre, B. and Guillaumin, S. (1997) Effect of High Ambient Temperature on Feed Digestibility in Broilers. Poultry Science, 76, 857-863.
[31]  Marder, J. and Arad, Z. (1989) Panting and Acid-Base Regulation in Heat Stressed Birds. Comparative Biochemistry and Physiology Part A, 94, 395-400.
[32]  Al-Zghoul, M.B., El-Bahr, S.M., Al-Rukibat, R.K., Dalab, A.S., Althnaian, T.A. and Al-Ramadan, S.Y. (2015) Biochemical and Molecular Investigation of Thermal Manipulation Protocols during Broiler Embryogenesis and Subsequent Thermal Challenge. BMC Veterinary Research, 11, Article No. 292.
[33]  Mahdavi, A.H., Rahman, A.R. and Pourreza, J. (2005) Effect of Probiotic Supplements on Egg Quality and Laying Hen’s Performance. International Journal of Poultry Science, 4, 488-492.
[34]  Soltan, M.A. (2008) Effect of Dietary Organic Acid Supplementation on Egg Production, Egg Quality and Some Blood Serum Parameters in Laying Hens. International Journal of Poultry Science, 7, 613-621.
[35]  Hajati, H. and Zaghari, M. (2019) Spirulina in Poultry Nutrition. Cambridge Scholars Publishing, Cambridge.
[36]  Mikulski, D., Jankowski, J., Naczmanski, J., Mikulska, M. and Demey, V. (2012) Effects of Dietary Probiotic (Pediococcus acidilactici) Supplementation on Performance, Nutrient Digestibility, Egg Traits, Egg Yolk Cholesterol, and Fatty Acid Profile in Laying Hens. Poultry Science, 91, 2691-2700.
[37]  Krysiak, K., Konkol, D. and Korczy′nski, M. (2021) Overview of the Use of Probiotics in Poultry Production. Animals, 11, Article No. 1620.
[38]  Ghazi, S.H., Habibian, M., Moeini, M.M. and Abdolmohammadi, A.R. (2012) Effects of Different Levels of Organic and Inorganic Chromium on Growth Performance and Immunocompetence of Broilers under Heat Stress. Biological Trace Element Research, 146, 309-317.
[39]  Felver-Gant, J.N., Mack, L.A., Dennis, R.L., Eicher, S.D. and Cheng, H.W. (2012) Genetic Variations Alter Physiological Responses Following Heat Stress in 2 Strains of Laying Hens. Poultry Science, 91, 1542-1551.
[40]  Dizaji, B.R., Sajjad, H. and Afshin, Z. (2012) Effects of Dietary Supplementations of Prebiotics, Probiotics, Synbiotics and Acidifiers on Growth Performance and Organs Weights of Broiler Chicken. European Journal of Experimental Biology, 2, 2125-2129.
[41]  Hidayat, M.N., Malaka, R., Agustina, L. and Pakiding, W. (2016) Abdominal Fat Percentage and Carcass Quality of Broiler Given Probiotics Bacillus spp. Metabolism: Clinical and Experimental, 22, 3-60.
[42]  Mohamadshahi, M., Veissi, M., Haidari, F., Shahbazian, H., Kaydani, G.A. and Mohammadi, F. (2014) Effects of Probiotic Yogurt Consumption on Inflammatory Biomarkers in Patients with Type 2 Diabetes. BioImpacts, 4, Article No. 83.
[43]  Wang, X., Farnell, Y.Z., Peebles, E.D., Kiess, A.S., Wamsley, K.G.S. and Zhai, W. (2016) Effects of Prebiotics, Probiotics, and Their Combination on Growth Performance, Small Intestine Morphology, and Resident Lactobacillus of Male Broilers. Poultry Science, 95, 1332-1340.
[44]  Dibner, J.J. and Richards, J. D. (2005) Antibiotic Growth Promoters in Agriculture: History and Mode of Action. Poultry Science, 84, 634-643.


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