Background: The accumulation of free radicals is linked to a number of diseases. Free radicals can be scavenged by antioxidants and reduce their harmful effects. It is therefore essential to look for naturally occurring antioxidants that come from plants, as synthetic antioxidants are toxic, carcinogenic and problematic for the environment. Lycopene is one of the carotenoids, a pigment that dissolves in fat and has antioxidant properties. Materials and Methods: The antioxidant and free radical scavenging activity were assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The impact of lycopene on bacteria (E. coli) susceptibility to γ-radiation was examined by radio sensitivity assay. The study also examined the induction of strand breaks in plasmid pUC19 DNA and how lycopene extract protected the DNA from γ-radiation in vitro. Results: At varying concentrations, lycopene demonstrated its ability to scavenge free radicals such as 2, 2-diphenyl-1-picrylhydrazyl (DPPH). IC50 for lycopene was determined at 112 μg/mL which was almost partial to IC50 of standard antioxidant L-ascorbic acid. The D10 value 180 Gy of E. coli was found to be >2-fold higher in the extract-containing lycopene sample than in the extract-free controls. The lycopene extracts inhibited the radiation-induced deterioration of the plasmid pUC19 DNA. At an IC50 concentration, lycopene provided the highest level of protection. Conclusion: Lycopene functions as an efficient free radical scavenger and possible natural antioxidant source. For cancer patients and others who frequently expose themselves to radiation, lycopene may be a useful plant-based pharmaceutical product for treating a variety of diseases caused by free radicals.
References
[1]
Lü, J.M., Lin, P.H., Yao, Q. and Chen, C. (2010) Chemical and Molecular Mechanisms of Antioxidants: Experimental Approaches and Model Systems. Journal of Cellular and Molecular Medicine, 14, 840-860. https://doi.org/10.1111/j.1582-4934.2009.00897.x
[2]
Kawamura, K., Qi, F. and Kobayashi, J. (2018) Potential Relationship between the Biological Effects of Low-Dose Irradiation and Mitochondrial ROS Production. Journal of Radiation Research, 59, ii91-ii97. https://doi.org/10.1093/jrr/rrx091
[3]
Hall, E.J. and Giaccia, A.J. (2005) Radiobiology for the Radiologist. 6th Edition, Lippincott Williams & Wilkins, Philadelphia.
[4]
Ravanat, J.L. and Douki, T. (2016) UV and Ionizing Radiations Induced DNA Damage, Differences and Similarities. Radiation Physics and Chemistry, 128, 92-102. https://doi.org/10.1016/j.radphyschem.2016.07.007
[5]
Fabre, K.M., Saito, K., DeGraff, W., Sowers, A.L., Thetford, A., Cook, J.A., Krishna, M.C. and Mitchell, J.B. (2011) The Effects of Resveratrol and Selected Metabolites on the Radiation and Antioxidant Response. Cancer Biology and Therapy, 12, 915-923. https://doi.org/10.4161/cbt.12.10.17714
[6]
Halliwell, B. (1995) How to Characterize an Antioxidant: An Update. Biochemical Society Symposia, 61, 73-101. https://doi.org/10.1042/bss0610073
[7]
Jayakumar, S., Pal, D. and Sandur, S.K. (2015) Nrf2 Facilitates Repair of Radiation Induced DNA Damage through Homologous Recombination Repair Pathway in a ROS Independent Manner in Cancer Cells. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 779, 33-45. https://doi.org/10.1016/j.mrfmmm.2015.06.007
[8]
Mai, A.A., Al Dajah, S., Murad, A.A., El-Salem, A.M. and Khafajah, A.M. (2019) Extraction, Purification, and Characterization of Lycopene from Jordanian Vine Tomato Cultivar, and Study of Its Potential Natural Antioxidant Effect on Samen Baladi. Current Research in Nutrition and Food Science, 7, 532-546. https://doi.org/10.12944/CRNFSJ.7.2.22
[9]
Papas, A.M. (1999) Diet and Antioxidant Status. Food and Chemical Toxicology, 37, 999-1007. https://doi.org/10.1016/S0278-6915(99)00088-5
[10]
Rao, A.V., Young, G.L. and Rao, L.G. (2018) Lycopene and Tomatoes in Human Nutrition and Health. CRC Press, Boca Raton. https://doi.org/10.1201/9781351110877
[11]
Gajowik, A. and Dobrzyńska, M.M. (2017) The Evaluation of Protective Effect of Lycopene against Genotoxic Influence of X-Irradiation in Human Blood Lymphocytes. Radiation and Environmental Biophysics, 56, 413-422. https://doi.org/10.1007/s00411-017-0713-6
[12]
Pathak, S., Akhade, P., Gajanan Bhojane, K., Sadar, D., Folane, P., Biyani, K.R. and Pathak, S.S. (2020) A Review on Recent Techniques of Extraction and Isolation of Lycopene from Tomato. International Journal of Research and Review, 7, 487-490.
[13]
Imran, M., Ghorat, F., Ulhaq, I., Urrehman, H., Aslam, F., Heydari, M., Shariati, M.A., Okuskhanova, E., Yessimbekov, Z., Thiruvengadam, M., Hashempur, M.H. and Rebezov, M. (2020) Lycopene as a Natural Antioxidant Used to Prevent Human Health Disorders. Antioxidants, 9, Article 706. https://doi.org/10.3390/antiox9080706
[14]
Islamian, J.P. and Mehrali, H. (2015) Lycopene as a Carotenoid Provides Radioprotectant and Antioxidant Effects by Quenching Radiation-Induced Free Radical Singlet Oxygen: An Overview. Cell Journal, 16, 386-391.
[15]
Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 Phage Cloning Vectors and Host Strains: Nucleotide Sequences of the M13mpl8 and pUC19 Vectors. Gene, 33, 103-119. https://doi.org/10.1016/0378-1119(85)90120-9
[16]
Roldán-Gutiérrez, J.M. and de Castro, M.D.L. (2007) Lycopene: The Need for Better Methods for Characterization and Determination. TrAC Trends in Analytical Chemistry, 26, 163-170. https://doi.org/10.1016/j.trac.2006.11.013
[17]
Brand-Williams, W. (1999) Use of a Free Radical Method to Evaluate Antioxidant Activity. Food Science and Technology, 28, 1231-1237.
[18]
Green, M.R. and Sambrook, J. (2012) Molecular Cloning: A Laboratory Manual. 4th Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
[19]
Dalgaard, P., Ross, T., Kamperman, L., Neumeyer, K. and McMeekin, T.A. (1994) Estimation of Bacterial Growth Rates from Turbidimetric and Viable Count Data. International Journal of Food Microbiology, 23, 391-404. https://doi.org/10.1016/0168-1605(94)90165-1
[20]
Madigan, M.T., Martinko, J.M. and Parker, J. (1997) Brock Biology of Microorganisms (Vol. 11). Prentice Hall, Upper Saddle River.
[21]
Alda, L.M., Gogoa, I., Bordean, D., Gergen, I., Alda, S., Moldovan, C. and Ni, L. (2009) Lycopene Content of Tomatoes and Tomato Products. Journal of Agroalimentary Process and Technologies, 15, 540-542.
[22]
Yamaguchi, T., Takamura, H., Matoba, T. and Terao, J. (1998) Hplc Method for Evaluation of the Free Radical-Scavenging Activity of Foods by Using 1, 1-Diphenyl-2-Picrylhydrazyl. Bioscience, Biotechnology and Biochemistry, 62, 1201-1204. https://doi.org/10.1271/bbb.62.1201
[23]
Fang, Y.Z., Yang, S. and Wu, G. (2002) Free Radicals, Antioxidants, and Nutrition. Nutrition, 18, 872-879. https://doi.org/10.1016/S0899-9007(02)00916-4
[24]
Xu, D.P., et al. (2017) Natural Antioxidants in Foods and Medicinal Plants: Extraction, Assessment and Resources. International Journal of Molecular Sciences, 18, 20-31. https://doi.org/10.3390/ijms18010096
[25]
Bunghez, I.R., Raduly, M., Doncea, S., Aksahin, I. and Ion, R.M. (2011) Lycopene Determination in Tomatoes by Different Spectral Techniques (UV-VIS, FTIR and HPLC). Digest Journal of Nanomaterials and Biostructures, 6, 1349-1356.
[26]
Rodriguez-Amaya, D.B. and Kimura, M. (2004) Harvest Plus Handbook for Carotenoid Analysis. International Food Policy Research Institute (IFPRI), Washington DC.
[27]
Ganesan, M., Rajesh, M., Solairaj, P. and Senthilkumar, T. (2012) Tomato as a Pioneer in Health Management. International Journal of Pharmaceutical, Chemical and Biological Sciences, 2, 210-217.
[28]
Borguini, R.G., Bastos, D.H.M., Moita-Neto, J.M., Capasso, F.S. and Torres, E.A.F.D.S. (2013) Antioxidant Potential of Tomatoes Cultivated in Organic and Conventional Systems. Brazilian Archives of Biology and Technology, 56, 521-529. https://doi.org/10.1590/S1516-89132013000400001
[29]
Viuda-Martos, M., Sanchez-Zapata, E., Sayas-Barberá, E., Sendra, E., Pérez-álvarez, J.A. and Fernández-López, J. (2014) Tomato and Tomato Byproducts. Human Health Benefits of Lycopene and Its Application to Meat Products: A Review. Critical Reviews in Food Science and Nutrition, 54, 1032-1049. https://doi.org/10.1080/10408398.2011.623799
[30]
Duthie, S.J., Ma, A., Ross, M.A. and Collins, A.R. (1996) Antioxidant Supplementation Decreases Oxidative DNA Damage in Human Lymphocytes. Cancer Research, 56, 1291-1295.
[31]
Wertz, K., Siler, U. and Goralczyk, R. (2004) Lycopene: Modes of Action to Promote Prostate Health. Archives of Biochemistry and Biophysics, 430, 127-134. https://doi.org/10.1016/j.abb.2004.04.023
[32]
Sharma, A., Gautam, S. and Jadhav, S.S. (2000) Spice Extracts as Dose-Modifying Factors in Radiation Inactivation of Bacteria. Journal of Agricultural and Food Chemistry, 48, 1340-1344. https://doi.org/10.1021/jf990851u
[33]
Kaviarasan, S., Naik, G.H., Gangabhagirathi, R., Anuradha, C.V. and Priyadarsini, K.I. (2007) In Vitro Studies on Antiradical and Antioxidant Activities of Fenugreek (Trigonella foenum graecum) Seeds. Food Chemistry, 103, 31-37. https://doi.org/10.1016/j.foodchem.2006.05.064
[34]
Kadam, U.S., Ghosh, S.B., De, S., Suprasanna, P., Devasagayam, T.P.A. and Bapat, V.A. (2008) Antioxidant Activity in Sugarcane Juice and Its Protective Role against Radiation Induced DNA Damage. Food Chemistry, 106, 1154-1160. https://doi.org/10.1016/j.foodchem.2007.07.066
[35]
Srinivasan, M., Sudheer, A.R., Pillai, K.R., Kumar, P.R., Sudhakaran, P.R. and Menon, V.P. (2007) Lycopene as a Natural Protector against γ-Radiation Induced DNA Damage, Lipid Peroxidation and Antioxidant Status in Primary Culture of Isolated Rat Hepatocytes in Vitro. Biochimica et Biophysica Acta (BBA)-General Subjects, 1770, 659-665. https://doi.org/10.1016/j.bbagen.2006.11.008
