Understanding metal accumulation at organ level in roots, leaves and seeds in O. glaberrima (OG) is crucial for improving physiological and metabolic aspects in growing Asian and African rice in salted areas. The micro-analytical imaging techniques are required to reveal its accumulation and distribution within plant tissues. PIXE studies have been performed to determine different elements in rice plants. The existing microbeam analytical technique at the iThemba LABS will be applied for the 2D image mapping of fresh rice tissues to perform a concentration of low atomic mass elements (such as Al, Si, P, S, Cl, Ca, Ti, Mn, Fe, Cu, Br, Zn and K) with detection limits of typically 1-10 μg/g. Comparison of the distribution of the elements between leaves, root and seed samples using uptake and distribution of elements in particular environmental conditions with potential amount of salt in water have been performed. We are also expecting to indicate metal exclusion as salt tolerance strategies from leaves, root, and seed compartments using matrix correlation between samples and between elements on rice species.
References
[1]
Sweeney, M. and McCouch, S. (2007) The Complex History of the Domestication of Rice. Annals of Botany, 100, 951-957. https://doi.org/10.1093/aob/mcm128
[2]
Jean-Pierre, L.G. (2002) Review of Carney, Judith A., Black Rice: The African Origins of Rice Cultivation in the Americas. H-South, H-Net Reviews.
[3]
Lidder, P. and Sonnino, A. (2012) Chapter 1—Biotechnologies for the Management of Genetic Resources for Food and Agriculture. Advances in Genetics, 78, 1-167. https://www.sciencedirect.com/science/article/pii/B9780123943941000018 https://doi.org/10.1016/B978-0-12-394394-1.00001-8
[4]
Katarina, V.M., Paula, P. and Primož, P. (2014) Micro-PIXE Elemental Mapping for Ionome Studies of Crop Plants. International Journal of PIXE, 24, 217-233. https://doi.org/10.1142/S0129083514400142
[5]
Kump, P. and Vogel-Mikuš, K. (2018) Quantification of 2D Elemental Distribution Maps of Intermediate-Thick Biological Sections by Low Energy Synchrotron Μ-X-Ray Fluorescence Spectrometry. Journal of Instrumentation, 13, C05014. https://doi.org/10.1088/1748-0221/13/05/C05014
[6]
Marijan, N., Peter, K., Janez, Š., Radojko, J., Jurij, S., Primož, P., Miloš, B., Zvonka, J., Paula, P., Marjana, R. and Katarina, V.M. (2008) Application of X-Ray Fluorescence Analytical Techniques in Phytoremediation and Plant Biology Studies. Spectrochimica Acta Part B: Atomic Spectroscopy, 63, 1240-1247. https://doi.org/10.1016/j.sab.2008.07.006
[7]
Bado, S., Padilla-Alvarez, R., Migliori, A., Forster, B.P., Jaksic, M., Diawara, Y., Kaiser, R. and Laimer, M. (2016) The Application of XRF and PIXE in the Analysis of Rice Shoot and Compositional Screening of Genotypes. NIM B: Beam Interactions with Materials and Atoms, 371, 407-412. https://doi.org/10.1016/j.nimb.2015.08.081
[8]
Seaman, C. (2017) Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Metabolites. Methods in Molecular Biology, 1618, 125-135. https://doi.org/10.1007/978-1-4939-7051-3_11
[9]
Vavpetič, P. (2020) Application of Micro-PIXE (Particle Induced X-Ray Emission) to Study Buckwheat Grain Structure and Composition. Fagopyrum, 37, 5-10. https://doi.org/10.3986/fag0012
[10]
Fernandes, S., Traore, A., Fleury, O., Havránek, V., Kučera, J. and Ndao, A.S. (2020) Multi-Elemental Analysis of Roots and Leaves from Oryza glaberrima Rice Plants at Vegetative Stage of Growth by Combined PIGE, RBS, PIXE and GC-TDS Methods. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 477, 109-115. https://doi.org/10.1016/j.nimb.2019.12.005
[11]
Peng, S.B., Tang, Q.Y. and Zou, Y.B. (2009) Current Status and Challenges of Rice Production in China. Plant Production Science, 12, 3-8. https://doi.org/10.1626/pps.12.3
[12]
Bidhan, R. and Sanjib, B. (2014) Effects of Toxic Levels of Aluminum on Seedling Parameters of Rice under Hydroponic Culture. Rice Science, 21, 217-223. https://doi.org/10.1016/S1672-6308(13)60182-1
[13]
Ryan, C.G., Jamieson, D.N., Griffin, W.L., Cripps, G. and Szymanski, R. (2001) The New CSIRO-GEMOC Nuclear Microprobe: First Results, Performance and Recent Applications. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 181, 12-19. https://doi.org/10.1016/S0168-583X(01)00548-1
[14]
Samal, P., Babu, S.C., Mondal, B. and Mishra, S.N. (2022) The Global Rice Agriculture towards 2050: An Inter-Continental perspective. Outlook on Agriculture, 51, 164-172. https://doi.org/10.1177/00307270221088338
[15]
Naseri, M., Vazirzadeh, A., Kazemi, R. and Zaheri, F. (2015) Concentration of Some Heavy Metals in Rice Types Available in Shiraz Market and Human Health Risk Assessment. Food Chemistry, 175, 243-248. https://doi.org/10.1016/j.foodchem.2014.11.109
[16]
Batista, B.L. (2012) Determination of Essential (Ca, Fe, I, K, Mo) and Toxic Elements (Hg, Pb) in Brazilian Rice Grains and Estimation of Reference Daily Intake. Food and Nutrition Sciences, 3, 129-134. https://doi.org/10.4236/fns.2012.31019
[17]
Moussa, N., Nathan, M., Masaki, O. and Fujio, K. (2018) Cadmium, Arsenic and Lead Accumulation in Rice Grains Produced in Senegal River. Fundamental Toxicological Sciences, 2, 740-741.
[18]
Williams, P.N., Price, A.H., Raab, A., Hossain, S.A., Feldmann, J. and Meharg, A.A. (2005) Variation in Arsenic Speciation and Concentration in Paddy Rice Related to Dietary Exposure. Environmental Science & Technology, 39, 5531-5540. https://doi.org/10.1021/es0502324
[19]
Meharg, A.A., Lombi, E., Williams, P.N., Scheckel, K.G., Feldmann, J., Raab, A. and Islam, R. (2008) Speciation and Localization of Arsenic in White and Brown Grains. Environmental Science & Technology, 42, 1051-1057. https://doi.org/10.1021/es702212p
