Bovine tuberculosis (bTB) is an endemic zoonosis significantly affects animal health in Burkina Faso. The primary causative agent is Mycobacterium tuberculosis (M. tuberculosis) complex, mainly M. bovis. Cattle are considered as natural reservoir of M. bovis. However, in Burkina Faso, the circulation of these strains remains poorly understood and documented. This study aimed to identify and characterize Mycobacterium strains from suspected carcasses during routine meat inspection at Bobo-Dioulasso refrigerated slaughterhouse. A prospective cross-sectional study was conducted from January 2021 to December 2022 on cases of seizures linked to suspected bovine tuberculosis. Microbiological and molecular analyzes were used for mycobacterial strain isolation and characterization. Out of 50 samples, 24% tested positive by microscopy and 12% by culture. Molecular analysis identified 6 strains of Mycobacteria, exclusively Mycobacterium bovis specifically the subspecies bovis (Mycobacterium bovis subsp bovis). In conclusion, M. bovis subsp bovis is the primary agent responsible for bovine tuberculosis in Bobo-Dioulasso. Continuous monitoring of mycobacterial strains is therefore necessary for the effective control of this pathology in the local cattle population.
References
[1]
World Organisation for Animal Health (2019). Zoonotic Tuberculosis in Mammals, Including Bovine and Caprine Tuberculosis. Organização Mundial de Saúde Animal (OIE). 1-20. https://www.cfsph.iastate.edu/
[2]
Rivière, J. and Riviere, M.J. (2016) Evaluation du dispositif de surveillance de la tuberculose bovine dans la faune sauvage en France à l’aide de méthodes épidémiologique, économique et sociologique. https://theses.hal.science/tel-01362997/document
[3]
Thoen, C., LoBue, P. and de Kantor, I. (2006) The Importance of Mycobacterium bovis as a Zoonosis. Veterinary Microbiology, 112, 339-345. https://doi.org/10.1016/j.vetmic.2005.11.047
[4]
Boussini, H., Traoré, A., Tamboura, H.H., Bessin, R., Boly, H. and Ouédraogo, A. (2012) Prevalence de la fuberculoseef de la brucellose dans les elevages bovins laifiers infra-urbains efperiurbains de la ville d’Ouagadougou au Burkina Faso. Revue Scientifique et Technique, 31, 943-951. https://doi.org/10.20506/rst.31.3.2168
[5]
Dibaba, A.B., Daborn, C.J., Cadmus, S. and Michel, A. (2019) Tuberculosis in Animals: An African Perspective. https://doi.org/10.1007/978-3-030-18690-6
[6]
Carruth, L., Roess, A.A., Mekonnen, Y.T., Melaku, S.K., Nichter, M. and Salman, M. (2016) Zoonotic Tuberculosis in Africa: Challenges and Ways Forward. Lancet, 388, 2460-2461. https://doi.org/10.1016/S0140-6736(16)32186-9
[7]
Kanyala, E., Shuaib, Y.A., Schwarz, N.G., Andres, S., Richter, E., Sawadogo, B., et al. (2022) Prevalence and Molecular Characterization of Mycobacterium bovis in Slaughtered Cattle Carcasses in Burkina Faso; West Africa. Microorganisms, 10, 1378. https://doi.org/10.3390/microorganisms10071378
[8]
DCRP/MARAH (2022) Première enquête nationale sur le cheptel (ENC1): Les résultats validés. https://www.agriculture.bf/jcms/pv10_104263/fr/premiere-enquete-nationale-sur-le-cheptel-enc1-les-resultats-valides
[9]
Sanou, A., Dicko, A., Sow, K.R., Djibougou, A., Kabore, A., Diarra, B., et al. (2021) Epidemiology and Microscopic Diagnosis of Tuberculosis in Pigs and Small Ruminants Slaughtered at Bobo-Dioulasso Abattoir, Burkina Faso. Onderstepoort Journal of Veterinary Research, 88, 1-6. https://doi.org/10.4102/ojvr.v88i1.1908
[10]
Tialla, D. and Ouedraogo, A. (2021) Tuberculose bovine et conséquences associées dans la commune rurale de Tanghin-Dassouri au Burkina Faso. Science et Technique, Sciences de la Santé, 44, 19-29.
[11]
Ejo, M., Haile, B., Tariku, T., Nigatu, S., Kebede, E., Bitew, A.B., et al. (2021) Bacteriological and Molecular Characterization of Mycobacterium bovis Isolates from Tuberculous Lesions Collected among Slaughtered Cattle, Northwest Ethiopia. BMC Microbiology, 21, Article No. 286. https://doi.org/10.1186/s12866-021-02349-1
[12]
Sibhat, B., Asmare, K., Demissie, K., Ayelet, G., Ameni, G., Dawa, D., et al. (2018) Bovine Tuberculosis in Ethiopia: A Systematic Review and Meta-Analysis. Preventive Veterinary Medicine, 147, 149-157. https://doi.org/10.1016/j.prevetmed.2017.09.006
[13]
Nuru, A., Zewude, A., Mohammed, T., Wondale, B., Teshome, L., Getahun, M., et al. (2017) Nontuberculosis Mycobacteria Are the Major Causes of Tuberculosis Like Lesions in Cattle Slaughtered at Bahir Dar Abattoir, Northwestern Ethiopia. BMC Veterinary Research, 13, Article No. 237. https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1168-3 https://doi.org/10.1186/s12917-017-1168-3
[14]
Tarnagda, Z., Kanyala, E., Zingué, D., Sidibé, S., Yougbaré, I., Méda, T.K.N., et al. (2014) Prevalence of Tuberculosis spp. Species in Bovine Carcasses in Two Slaughterhouses of Burkina Faso. International Journal of Microbiology and Immunology Research, 2, 92-100.
