Up-Regulated Expression of SOD2 and HPRT1 Following Topical Photoprotection and Photorepair Skincare Formulations in A 3-Dimensional Reconstructed Human Skin Model
Photodamage
continues to threaten human skin health despite worldwide sun awareness campaigns and the widespread use of sunscreens.To date, extensive research is lacking into the effects of sun avoidance
and solar specific skincare regimens on gene expression changes and DNA repair
activity. We have previously reported that photoprotection and photorepair
formulations which minimize the harmful effects of ultraviolet, visible light
and near-infrared radiation can provide photoprotection, anti-photoaging
benefits and rejuvenating effects optically, clinically and genetically. To
investigate gene expression changes, specifically antioxidant and DNA repair
effects following the use of topical photoprotection and photorepair
formulations (The Essential Six, RATIONALE, Victoria, Australia), we used
epidermal keratinocytes and dermal fibroblasts derived from a 3-dimensional
reconstructed human skin model, and assessed upregulation of SOD2 and HPRT1.
Gene expression was assessed via the Genemarkers Standard Skin Panel and
quantitative real-time PCR exploration. Tissues were inoculated with solar specific topical formulations, then collected after 24 hours following application of
photoprotection formulations and 16 hours following photorepair formulations.
The quantitative real-time PCR revealed that, in comparison to the control, the
genes encoding SOD2 and HPRT1 have been significantly up-regulated following usage of the photoprotection
formulations, 1.86, and 1.41, respectively. SOD2 and HPRT1 were up-regulated following use of the photorepair formulations,
2.15, and 1.28, respectively. We were able to substantiate that the photo
protection and photorepair formulations upregulated genes
involved in antioxidant and DNA repair mechanisms in a 3-dimensional
reconstructed human skin model, suggesting a promising anti-photoaging skin
regimen.
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