|
β-罗勒烯对烟草马铃薯Y病毒防控效果的研究
|
Abstract:
β-罗勒烯是一种能诱导植物产生防御反应的植物通讯信号分子。本文为了探究β-罗勒烯对烤烟马铃薯Y病毒病(PVY)的控制效果,在室内条件下,分析了β-罗勒烯对PVY的发病率及病情指数的影响;在大田条件下,同时分析了β-罗勒烯与常规化学农药对PVY的防控效果。试验结果如下:通过室内盆栽试验分析,β-罗勒烯对PVY具有显著的防控效果,相比于对照发病率下降了67.10%,病情指数下降了70.60%,病毒积累量减少了1.7倍。大田试验结果显示,β-罗勒烯对PVY的控制效果显著优于化学农药,对PVY的控制效果达到58.23%~64.12%;进一步对PVY病情上升率进行统计,结果表明,经β-罗勒烯诱导后PVY的病情指数仅上升了10.0%,显著高于化学农药及对照。以上结果均表明,β-罗勒烯在防控PVY方面具有显著功效。本研究为大田防控PVY提供了新的途径。
β-ocimene is a plant communication signaling molecule that can induce plant defense responses. In this paper, in order to investigate the control effect of β-ocimene on tobacco Potato virus Y (PVY) disease, under indoor conditions, the influence of β-ocimene on the incidence rate and disease index of PVY was analyzed; under field conditions, the prevention and control effects of β-ocimene and conventional chemical pesticides on PVY were analyzed simultaneously. The test results are as follows: According to the analysis of indoor pot experiment, β-ocimene has a significant prevention and control effect on PVY. Compared with the control, the incidence rate decreased by 67.10%, the disease index decreased by 70.60%, and the virus accumulation decreased by 1.7 times. The results of field experiments showed that the control effect of β-ocimene on PVY was significantly better than that of chemical pesticides, with a control effect of 58.23%~64.12% on PVY; further statistical analysis of the increase rate of PVY showed that the disease index of PVY induced by β-ocimene only in-creased by 10.0%, significantly higher than that of chemical pesticides and controls. The above results all indicate that β-ocimene has significant efficacy in preventing and controlling PVY. This study provides a new approach to the prevention and control of PVY in the field.
[1] | Chen, S., Li, F.X., Liu, D., et al. (2017) Dynamic Expression Analysis of Early Response Genes Induced by Potato vi-rus Y in PVY-Resistant Nicotiana tabacum. Plant Cell Reports, 36,297-311.
https://doi.org/10.1007/s00299-016-2080-1 |
[2] | 张凤桐, 程林发, 耿超, 等. 一株PVY[NTN-NW]黑龙江马铃薯分离物的检测鉴定[J]. 植物病理学报, 2019, 49(4): 512-519. |
[3] | 夏范讲, 郭玉双, 贾蒙骜. 贵州烟田马铃薯Y病毒优势株系全序列克隆及系统进化分析[J]. 中国农业大学学报, 2017, 22(7): 512-519. |
[4] | 谈文, 刘骏. 烟草病毒病发生流行概况及综合治理的建议[J]. 烟草科技, 1987(2): 33-36. |
[5] | 段丽娟. 烟草病毒病的发生与防治技术[J]. 现代化农业, 2006(2): 15-16. |
[6] | 蔡海林, 何命军, 谢鹏飞, 等. 烟草马铃薯Y病毒病的生物防治研究进展[J]. 湖南农业科学, 2012(8): 76-79. |
[7] | 周佳, 李凤霞, 陈帅, 等. 烟草抗马铃薯Y病毒病相关基因NtPsaN的克隆及表达分析[J]. 中国农业科学, 2010, 43(16): 3323-3330. |
[8] | 饶思莲, 陶永萍, 赵声春, 等. 烟草抗PVY新品系比较试验[J]. 分子植物育种, 2018, 16(10): 3401-3411. |
[9] | 刘勇, 许美玲, 黄昌军, 等. 抗马铃薯Y病毒病的烟草种质资源抗性分析[J]. 分子植物育种, 2016, 14(5): 1212-1216. |
[10] | Morel, J.-B. and Dang, J.L. (1997) The Hypersensitive Response and the Induction of Cell Death in Plants. Cell Death and Differentiation, 4, 671-683. https://doi.org/10.1038/sj.cdd.4400309 |
[11] | Durrant, W.E. and Dong, X. (2004) Systemic Acquired Resistance. Annual Review of Phytopathology, 42, 185-209.
https://doi.org/10.1146/annurev.phyto.42.040803.140421 |
[12] | 肖牧. 罗勒烯诱导植物防御反应的分子机制研究[D]: [博士学位论文]. 长沙: 湖南农业大学, 2019. |
[13] | Liu, C.L., Ruan, Y., Guan, C.Y., et al. (2004) β-Ocimene Gene Expression Pattern Induced by Ocimene Signaling Molecule. Science Bulletin, 22, 2373-2374. |
[14] | Ramegowda, V., Mysore, K.S. and Senthi-Kumar, M. (2014) Virus-Induced Gene Silencing Is a Versatile Tool for Unraveling the Functional Relevance of Multiple Abiotic-Stress-Responsive Genes in Crop Plants. Frontiers in Plant Science, 5, Article 323. https://doi.org/10.3389/fpls.2014.00323 |