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基于抗菌活性的银杏黄酮树脂吸附分离研究
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Abstract:
鉴于银杏黄酮具有较强抗菌活性，本研究以银杏叶黄酮提取物为原料，选用大孔树脂填料进行分离提纯研究；通过吸附解吸实验，确定NKA-9大孔树脂适用于纯化银杏叶黄酮；后续优选NKA-9纯化条件为：提取物黄酮浓度0.8 mg/mL，pH为5，上样流速0.6 BV/h，用70%的乙醇溶液以1.0 BV/h流速洗脱，洗脱剂用量4 BV。纯化后的黄酮纯度为57.41%。通过大孔树脂对其纯化，为后续抗菌活性、抑菌机理进行探究提供物质基础。
Ginkgo biloba flavonoids has strong antibacterial activity. In this paper, authors used Ginkgo biloba leaf flavonoids extract as raw material and selected macroporous resin fillers for separation and purification. Through adsorption and desorption experiments, it was determined that NKA-9 MR was suitable for purifying Ginkgo biloba flavonoids. The purification conditions of NKA-9 were as follows: the concentration of flavonoids was 0.8 mg/mL, pH was 5, and the loading flow rate was 0.6 BV/h. The eluent was eluted with 70% ethanol solution at 1.0 BV/h with a dosage of 4 BV. The purity of purified flavonoids was 57.41%. It was purified by macroporous resin to provide material basis for further investigation of antibacterial activity and antibacterial mechanism.
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