The conditional mutations in drosophila were
obtained by γ-irradiation and selected using the test for dominant
lethality. The conditional mutations survive under permissive genetic
conditions and, additionally, commence to display novel properties. One of such
properties is a recessive lethality. Ten conditional mutations that displayed
recessive lethality were mapped with the help of a standard set of deletions.
Half mutations contained two and more lethal defects. The fact that a large
number of the lethal defects are associated with one mutation suggests that γ-irradiation
is the most unlikely cause of the defects. One of the conditional mutations
carried four lethal regions and had a Small barrel (Smba) visual phenotype. The
Smba phenotype in the Smba/In(2LR) Cy strain is inherited according to a
parental type and disappears in the Smba/In(2LR) Pm strain. Lethality in two of
the four lethal regions also disappears in this strain. A separate experiment
was conducted to clarify how these regions lost a lethal manifestation after
the In(2LR) Cy chromosome in the Smba/In(2LR) Cy
strain was replaced with the In(2LR) Pm chromosome. The process of
disappearance of the Smba phenotype was also observed in three Smba/In(2LR) Cy substocks. These data suggest
that the regions of multiple recessive lethality emerge in a secondary
manner under the effect of the earlier formed radiation-induced mutation in
ontogene. It is assumed that the recessive lethal regions are the ontogenes
with an altered DNA conformation. The conformation in ontogenes is changed in
the germline cells during a regular “editing” of the individual development
program.
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