全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

A Novel Facial Cream Based on Skin Penetrable Hemp Oil Microparticles

DOI: 10.4236/jcdsa.2023.133015, PP. 165-178

Keywords: Hemp, Microparticles, Anti-Inflammatory, Antioxidant, Skin Penetration

Full-Text   Cite this paper   Add to My Lib

Abstract:

Objective: Hemp seed oil is perfect for most skin types; it moisturizes skin and protects it from inflammation, oxidation, and other causes of aging. The problem is that the Hemp oil-based products do not penetrate the skin; they remain on the skin’s surface. Recently researchers have been trying to prepare nano emulsions of hemp oil to facilitate its permeation to deep skin layers. In all techniques used today, surfactants are added to the emulsification process. These surfactants may cause unwanted skin side effects. In the present study, we prepare micronized Hemp (m-Hemp) without using any surfactants in the micronization process, thus avoiding the side effects associated with surfactant addition. Methods & Results: Particles size of m-Hemp was evaluated using electron microscopy. Various sizes of m-Hemp were found, the smallest being 100 nm in diameter. The antioxidation properties of m-Hemp were measured using the Electron Spin Resonance (ESR) technique and were found to be enhanced. Skin topography and morphology following a cream containing m-Hemp treatment were visualized by Optical Profilometry and ESEM respectively. The results show a marked improvement in skin topography in all measured parameters. In addition, human keratinocytes (HaCaT) were exposed to inflammatory conditions and were then treated using Hemp. As a result, one of the key inflammatory factors (IL-2) was significantly reduced after treatment with m-Hemp (p ≤ 0.0001). The skin penetration of the cream containing m-Hemp was tested on human skin using the IMOPE (Iterative Multi-plane Optical Property Extraction) system. The results indicate that m-Hemp penetrates both the stratum corneum and the deep epidermal layers towards the dermis. Conclusion: The new cream prepared with micronized Hemp shows significant anti-inflammatory and antioxidative effects and demonstrates the entrance of m-Hemp to the skin epidermal layer.

References

[1]  Hazekamp, A., Fischedick, J.T., Díez, L., Lubbe, A. and Ruhaak, R L. (2010) Chemistry of Cannabis. In: Liu, H.-W. and Mander, L., Eds., Comprehensive Natural Products II., Elsevier, Oxford, 1033-1084.
[2]  Vitorović J., Joković, N., Radulović, N., Mihajilov-Krstev, T., Cvetković, V.J., Jovanović, N., Mitrović, T., Aleksić, A., Stanković, N. and Bernstein, N. (2021) Antioxidant Activity of Hemp (Cannabis sativa L.) Seed Oil in Drosophila melanogaster Larvae under Non-Stress and H2O2-Induced Oxidative Stress Conditions. Antioxidants, 10, Article 830. https://doi.org/10.3390/antiox10060830
[3]  Smeriglio, A., Galati, E.M., Monforte, M.T., Lanuzza, F., D'Angelo, V. and Circosta, C. (2016) Polyphenolic Compounds and Antioxidant Activity of Cold-Pressed Seed Oil from Finola Cultivar of Cannabis sativa L. Phytotherapy Research, 30, 1298-1307. https://doi.org/10.1002/ptr.5623
[4]  Sapino, S., Carlotti, M.E., Peira, E. and Gallarate, M. (2005) Hemp-Seed and Olive Oils: Their Stability against Oxidation and Use in O/W Emulsions. Journal of Cosmetic Science, 56, 227-251.
[5]  Rezapour-Firouzi, S., Mohammadian, M., Sadeghzadeh, M., Mehranfar, S. and Mazloomi, E. (2020) The Effects of Evening Primrose/Hemp Seed Oil Compared to Rapamycin on the Gene Expression of Immunological Parameters in Experimental Autoimmune Encephalomyelitis Splenocytes. The Iranian Journal of Allergy, Asthma and Immunology, 19, 183-192.
[6]  Jin, S. and Lee, M.Y. (2018) The Ameliorative Effect of Hemp Seed Hexane Extracts on the Propionibacterium Acnes-Induced Inflammation and Lipogenesis in Sebocytes. PLOS One, 13, e0202933. https://doi.org/10.1371/journal.pone.0202933
[7]  Vaughn, A.R., Clark, A.K., Sivamani, R.K. and Shi, V.Y. (2018) Natural Oils for Skin-Barrier Repair: Ancient Compounds Now Backed by Modern Science. American Journal of Clinical Dermatology, 19, 103-117.
https://doi.org/10.1007/s40257-017-0301-1
[8]  Kapoor, R. and Huang, Y.S. (2006) Gamma Linolenic Acid: An Anti-Inflammatory Omega-6 Fatty Acid. Current Pharmaceutical Biotechnology, 7, 531-534.
https://doi.org/10.2174/138920106779116874
[9]  Costedoat, M.R.S. and Wepierre, J. (1981) Percutaneous Absorption and Distribution of (14) C-Gamma-Linolenic Acid in Hairless Rats and Man. International Journal of Cosmetic Science, 3, 83-93.
[10]  Tabassum, N. and Hamdani, M. (2014) Plants Used to Treat Skin Diseases. Pharmacognosy Reviews, 8, 52-60. https://doi.org/10.4103/0973-7847.125531
[11]  Fryd, M.M. and Mason, T.G. (2012) Advanced Nanoemulsions. The Annual Review of Physical Chemistry, 63, 493-518.
https://doi.org/10.1146/annurev-physchem-032210-103436
[12]  Badmus, S.O., Amusa, H.K., Oyehan, T.A. and Saleh T.A. (2021) Environmental Risks and Toxicity of Surfactants: Overview of Analysis, Assessment, and Remediation Techniques. Environmental Science and Pollution Research, 28, 62085-62104.
https://doi.org/10.1007/s11356-021-16483-w
[13]  Yuan, C.L., Xu, Z.Z., Fan, M.X., Liu, H.Y., Xie, Y.H. and Zhu, T. (2014) Study on Characteristics and Harm of Surfactants. Journal of Chemical and Pharmaceutical Research, 6, 2233-2237.
[14]  Seweryn, A. (2018) Interactions between Surfactants and the Skin—Theory and Practice. Advances in Colloid and Interface Science, 256, 242-255.
https://doi.org/10.1016/j.cis.2018.04.002
[15]  Kahremany, S., Hofmann, L., Eretz-Kdosha, N., Silberstein, E., Gruzman, A. and Cohen, G. (2021) SH-29 and SK-119 Attenuates Air-Pollution Induced Damage by Activating Nrf2 in HaCaT Cells. International Journal of Environmental Research and Public Health, 18, Article 12371. https://doi.org/10.3390/ijerph182312371
[16]  Kahremany, S., Babaev, I., Gvirtz, R., Ogen-Stern, N., Azoulay-Ginsburg, S., Senderowitz, H., Cohen, G. and Gruzman, A. (2019) Nrf2 Activation by SK-119 Attenuates Oxidative Stress, UVB, and LPS-Induced Damage. Skin Pharmacology and Physiology, 32, 173-181. https://doi.org/10.1159/000499432
[17]  Li, C., Liu, W., Wang, F., Hayashi, T., Mizuno, K., Hattori, S., Fujisaki, H. and Ikejima, T. (2021) DNA Damage-Triggered Activation of cGAS-STING Pathway Induces Apoptosis in Human Keratinocyte HaCaT Cells. Molecular Immunology, 131, 180-190. https://doi.org/10.1016/j.molimm.2020.12.037
[18]  Blanchard, G., Pich, C. and Hohl, D (2020) HaCaT Cells As a Model System to Study Primary Cilia in Keratinocytes. Experimental Dermatology, 31, 1276-1280.
[19]  Wei, M., Liu, F., Raka, R., Xiang, J., Xiao, J., Han, T., Guo, F., Yang, S. and Wu. H. (2022) In vitro and in silico Analysis of “Taikong Blue” Lavender Essential Oil in LPS-Induced HaCaT Cells and RAW 264.7 Murine Macrophages. BMC Complementary Medicine and Therapies, 22, Article No. 324.
https://doi.org/10.1186/s12906-022-03800-0
[20]  Lee, K.J., Ratih, K., Kim, G.J., Lee, Y.R., Shin, J.S., Chung, K.H., Choi, E.J., Kim, E.K. and An, J.H. (2022) Immunomodulatory and Anti-Inflammatory Efficacy of Hederagenin-Coated Maghemite (γ-Fe2O3) Nanoparticles in An Atopic Dermatitis Model. Colloids and Surfaces B: Biointerfaces, 210, Article 112244.
https://doi.org/10.1016/j.colsurfb.2021.112244
[21]  Ying, Z., Li, X., Dang, H., Yin, N. and Gao, C. (2020) Molecular Immune Mechanisms of HPV-infected HaCaT Cells in vitro Based on Toll-Like Receptors Signaling Pathway. Journal of Clinical Laboratory Analysis, 34, e23101.
https://doi.org/10.1002/jcla.23101
[22]  Yariv, I., Rahamim, Shliselberg, E.G., Duadi, H., Lipovsky, A., Lubart, R. and Fixler, D. (2014) Detecting Nanoparticles in Tissue Using An Optical Iterative Technique. Biomedical Optics Express, 5, 3871-3881. https://doi.org/10.1364/BOE.5.003871
[23]  Lamprecht, M.R., Sabatini, D.M. and Carpenter, A.E. (2007) Cell Profiler: Free, Versatile Software for Automated Biological Image Analysis. Biotechniques, 42, 71-75. https://doi.org/10.2144/000112257
[24]  Yariv, I., Haddad, M., Duadi, H., Motiei, M. and Fixlr, D. (2016) New Optical Sensing Technique of Tissue Viability and Blood Flow Based on Nanophotonic Iterative Multi-Plane Reflectance Measurements. International Journal of Nanomedicine, 11, 5237-5244. https://doi.org/10.2147/IJN.S119130
[25]  Yariv, I., Duadi, H., Chakraborty, R. and Fixler, D. (2019) Algorithm for in vivo Detection of Tissue Type from Multiple Scattering Light Phase Images. Biomedical Optics Express, 10, 2909-2917. https://doi.org/10.1364/BOE.10.002909
[26]  Yariv, I., Duadi, H. and Fixler, D. (2020) Depth Scattering Characterization of Multi-Layer Turbid Media Based on Iterative Multi-Plane Reflectance Measurements. IEEE Photonics Journal, 12, Article 3700713.
https://doi.org/10.1109/JPHOT.2020.3017697
[27]  Yariv, I., Kannan, S., Harel, Y., Levy, E., Duadi, H., Lellouche, J.P., Michaeli, S. and Fixler, D. (2021) Iterative Optical Technique for Detecting Anti-Leishmania Nanoparticles in Mouse Lesions. Biomedical Optics Express, 12, 4496-4509.
https://doi.org/10.1364/BOE.425798
[28]  Yariv, I., Duadi, H. and Fixler, D. (2019) An Optical Method to Detect Tissue Scattering: Theory, Experiments and Biomedical Applications. Proceedings of the SPIE, 10891, Article 1089105. https://doi.org/10.1117/12.2508335
[29]  Bashkatov, A.N., Genina, E.A. and Tuchin, V.V. (2011) Optical Properties of Skin, Subcutaneous, and Muscle Tissues: A Review. Journal of Innovative Optical Health Sciences, 4, 9-38. https://doi.org/10.1142/S1793545811001319
[30]  Sharma, S., Shukla, P., Misra, A. and Mishra, P.R. (2014) Chapter8-Interfacial and Colloidal Properties of Emulsified Systems: Pharmaceutical and Biological Perspective. Colloid Interface Science in Pharmaceutical Research and Development, 2014, 149-172. https://doi.org/10.1016/B978-0-444-62614-1.00008-9

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133