The fixation of leaves of Tanacetum vulgare L. in glutaraldehyde
makes itpossible to isolate chloroplasts without
sacrificing an electron dense substance in the thylakoid lumen. The
extraction of lipids from the chloroplasts isolated from the leaves preliminarily
fixed in glutaraldehyde has demonstrated that glycerolipids (galactolipids and
phospholipids) are not manifested in TLC, whereas isoprenoid lipids
(chlorophyll, carotenoids) are manifested. Presumably, isoprenoid lipids are
not fixed with glutaraldehyde and are extracted from the thylakoid membrane.
The ultrastructural control demonstrates that the electron dense substance from
the thylakoid lumen is also extracted. It is possible that this substance is of
isoprenoid nature.
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