|
|
李比利氏灰粉蚧的分子鉴定方法及截获分析
|
Abstract:
本文对来自于印度尼西亚截获的山竹果上的粉蚧进行了分子和形态学鉴定，其结果确定为李比利氏灰粉蚧Dysmicoccus lepelleyi (Betrem)。介绍了其分子生物学鉴定方法，通过分子比对可准确鉴定其种类，并通过构建系统发育树确定了其与几个近缘种的关系，拍摄了本次截获粉蚧的生态照及玻片标本照。对海关检疫系统截获的李比利氏灰粉蚧进行了查询，分析了该虫的截获规律。
Both morphological characteristics and DNA barcoding were used to compare and identify the mealybug in mangostens intercepted from Indonesia in this study. The mealybug is identified as Dysmicoccus lepelleyi. The species can be accurately identified by molecular comparison, and the relationship between the species and several related species can be determined by constructing phylogenetic tree. Habitus photos and slide specimen photos of the mealybug were taken. The pattern of Dysmicoccus lepelleyi intercepted by customs quarantine system was analyzed.
| [1] | 廖力, 徐淼锋, 张卫东, 等. 应用DNA条形码技术鉴别谷实夜蛾与棉铃虫[J]. 植物检疫, 2012, 26(6): 12-16. |
| [2] | Park, D.S., Suh, S.J., Hebert, P.D.N., et al. (2011) DNA Barcodes for Two Scale Insect Families, Mealybugs (Hemiptera: Pseudococcidae) and Armored Scales (Hemiptera: Diaspididae). Bulletin of Entomological Research, 101, 429-434. https://doi.org/10.1017/S0007485310000714 |
| [3] | Kumar, S., Stecher, G. and Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870-1874. https://doi.org/10.1093/molbev/msw054 |
| [4] | 李惠萍, 杜智欣, 董文勇, 等. SN/T 4995-2017李比利氏灰粉蚧检疫鉴定方法[S]. 北京: 中国标准出版社, 2017. |
| [5] | 焦懿, 余道坚, 陈志粦, 等. 从进口泰国、印度尼西亚和马来西亚山竹上截获重要害虫李比利氏灰粉蚧[J]. 植物检疫, 2011, 25(2): 59-61. |
| [6] | Williams, D.J. (2004) The Mealybugs of Southern Asia. Southdene, Kuala Lumpur, 195-200. |
| [7] | 詹国平, 钟勇, 马晨, 等. SN/T 5543-2022李比利氏灰粉蚧检疫辐照处理最低吸收剂量[S]. 北京: 中国海关出版社, 2022. |
